Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells

Gaude, Nicole; Bortfeld, Silvia; Erban, Alexander; Kopka, Joachim; Krajinski, Franziska

doi:10.1186/s12870-015-0601-7

Cited by 21 publications

(14 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased numbers of mitochondria and plastids lead to increased energy production (from the TCA cycle) and production of plastid metabolites (fatty acids, amino acids, carotenoids, and terpenoids) respectively ( Lohse et al, 2005 ; Jung et al, 2012 ). In the cytosol, sugar levels increase due to increased photosynthesis in the above-ground leaves, which favors high efflux rates between the arbuscule and host cell ( Smith and Gianinazzi-Pearson, 1988 ; Berger et al, 2007 ; Gaude et al, 2015 ). Levels of jasmonic acid (derived from linoleic acid produced in the plastids) also increase and trigger the production of phytoalexins (defensive compounds).…”

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

“…As these metabolic changes occur, phosphorus is transferred from the mycorrhiza to the host cell in exchange for fatty acids, amino acids, and sugars (fructose and glucose) ( Smith and Gianinazzi-Pearson, 1988 ). The production of anti-fungal compounds (e.g., gallic acid) by the host plant decreases ( Gaude et al, 2015 ).…”

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

“…Arbuscular mycorrhizal fungi symbiosis causes both global (species-independent) and local (species-specific) changes in plant metabolism. Global changes include increased production of amino acids (glutamic, aspartic, and asparagine acid); fatty acids (palmitic and oleic); secondary metabolites (phenyl alcohols, a linolenic acid, apocarotenoids, isoflavonoids); plant hormones (oxylipin, cytokinins, and jasmonic acid); activation of the oxylipin pathway; and increased sugar metabolism ( Fernández et al, 2014 ; Gaude et al, 2015 ; Rivero et al, 2015 ). In contrast, levels of specific secondary compounds increase according to plant species identity.…”

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

See 2 more Smart Citations

Engineering Mycorrhizal Symbioses to Alter Plant Metabolism and Improve Crop Health

French

2017

Front. Microbiol.

View full text Add to dashboard Cite

Creating sustainable bioeconomies for the 21st century relies on optimizing the use of biological resources to improve agricultural productivity and create new products. Arbuscular mycorrhizae (phylum Glomeromycota) form symbiotic relationships with over 80% of vascular plants. In return for carbon, these fungi improve plant health and tolerance to environmental stress. This symbiosis is over 400 million years old and there are currently over 200 known arbuscular mycorrhizae, with dozens of new species described annually. Metagenomic sequencing of native soil communities, from species-rich meadows to mangroves, suggests biologically diverse habitats support a variety of mycorrhizal species with potential agricultural, medical, and biotechnological applications. This review looks at the effect of mycorrhizae on plant metabolism and how we can harness this symbiosis to improve crop health. I will first describe the mechanisms that underlie this symbiosis and what physiological, metabolic, and environmental factors trigger these plant-fungal relationships. These include mycorrhizal manipulation of host genetic expression, host mitochondrial and plastid proliferation, and increased production of terpenoids and jasmonic acid by the host plant. I will then discuss the effects of mycorrhizae on plant root and foliar secondary metabolism. I subsequently outline how mycorrhizae induce three key benefits in crops: defense against pathogen and herbivore attack, drought resistance, and heavy metal tolerance. I conclude with an overview of current efforts to harness mycorrhizal diversity to improve crop health through customized inoculum. I argue future research should embrace synthetic biology to create mycorrhizal chasses with improved symbiotic abilities and potentially novel functions to improve plant health. As the effects of climate change and anthropogenic disturbance increase, the global diversity of arbuscular mycorrhizal fungi should be monitored and protected to ensure this important agricultural and biotechnological resource for the future.

show abstract

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

Section: Mycorrhizal Infection and Host Metabolic Responsementioning

confidence: 99%

See 1 more Smart Citation

Engineering Mycorrhizal Symbioses to Alter Plant Metabolism and Improve Crop Health

French

2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Arbuscular mycorrhizal symbiosis alters plant metabolism, including low molecular organic acids (glutamic, aspartic, asparagine, palmitic and oleic acid), secondary metabolites (phenyl alcohols, a-linolenic acid, apocarotenoids and isoflavonoids), plant hormones (oxylipin, cytokinins and jasmonic acid) and others, which affect soil microorganisms in direct or indirect ways [49][50][51]. The production of these substances are highly variable within and between different types of mycorrhizal fungi and are influenced by environmental conditions [52].…”

Section: Discussionmentioning

confidence: 99%

Mycorrhizal Root Exudates Induce Changes in the Growth and Fumonisin Gene (FUM1) Expression of Fusarium proliferatum

2019

View full text Add to dashboard Cite

In this study, root exudates from mycorrhizal and non-mycorrhizal plants growing at low or high nutrient supply were used in vitro to examine their effects on the growth and fumonisin B1 gene (FUM1) expression of Fusarium proliferatum (Hypocreales: Nectriaceae). After one day of exposure to root exudates originating from non-mycorrhizal and low nutrient supply treatment, a significant change in the growth of F. proliferatum was measured, which then equalized after 5 days of incubation. Aside from the fumonisin gene (FUM1) gene, the expression of the mitogen-activated protein kinase gene (HOG1) was also studied using quantitative real-time polymerase chain reaction (qRT-PCR). After 5 days of incubation, mycorrhizal root exudates significantly reduced the expression of the FUM1 gene, irrespective of the extent of the nutrient supplement and colonization level of the target plant. Similar trends in the expressions of FUM1 and HOG1 genes found in our experiment suggest that arbuscular mycorrhizal fungal colonization did not only affect directly the growth and mycotoxin production of F. proliferatum, but also modulated indirectly a number of other mechanisms. Mycorrhizal inoculation showed potential as a biological control agent in the suppression of fumonisin production by F. proliferatum.Root exudates were collected from maize plants treated, as described previously, by removing all substrate particles from the roots and then submerging them into 50 mL of 0.01 M L −1 KOH according to da Silva Lima et al. [38]. After 5 min, the root system was washed with tap water, then with Agronomy 2019, 9, 291 3 of 11 distilled water and were incubated in Erlenmeyer flasks filled with 50 mL sterilized distilled water for 24 h. Solutions were sterilized by filtration through 0.22 µm Ø nitrocellulose filters (Millipore, Burlington, MA, USA). Concentrations of root exudates were adjusted to a ratio of 1 g of root fresh weight based on the method of Lioussanne et al. [39]. In this way, the effects of various root exudates remained comparable, however, roots from different treatments showed significant differences in weights. The root exudates were kept at −20 • C until use. Culture Conditions and Expression Analyses of FUM1 and HOG1 GenesFusarium proliferatum ITEM 2287 (Institute of Sciences of Food Production, CNR, Bari, Italy) was used in the study. Mycelium for fumonisin production was prepared in 50 mL DM (22 mM KH 2 PO 4 , 2.5 mM MgSO 4 , 85 mM NaCl and 117 mM sucrose, pH 5.9) according to Shim and Woloshuk [40], supplemented with 30 mM ammonium dihydrogen phosphate and inoculated with 10 6 mL −1 conidia. Cultures were incubated at 26 • C with shaking (150 rpm) for 48 h then filtrated and washed with sterilized distilled water and mycelium, then transferred directly to 50 mL of new DM medium. The co-cultures were inoculated separately with 5 mL (concentrations adjusted to a ratio of 1 g of root fresh weight) of each of root exudate (+AM LN, −AM LN, +AM HN, −AM HN). The control treatment was prepared by adding 5 mL of sterilized ...

show abstract

“…Effects of nutrient deficiency on the transcriptome of both partners at the biotrophic interface are poorly characterized (Bonneau et al, 2013 ; Wipf et al ). Increasing applications and improvements of methodologies could complete classical transporter characterization and give a better detection and resolution of the functioning of biotrophic interfaces: transcripts and proteins by laser capture microdissection technology (Koegel et al, 2013 ), nutrients by NanoSIMS (Kaiser et al, 2015 ), and metabolites by liquid/gas chromatography–mass spectrometry (Gaude et al, 2015 ; Rivero et al ). These recent technological achievements in model plants associated to microbial consortia will facilitate comprehensive identification of the key nutrient transporters involved in biotrophic (mutualistic and pathogenic) interactions.…”

mentioning

confidence: 99%

Editorial: Transport in Plant Microbe Interactions

Courty

Wipf

2016

Front. Plant Sci.

View full text Add to dashboard Cite

Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells

Cited by 21 publications

References 67 publications

Engineering Mycorrhizal Symbioses to Alter Plant Metabolism and Improve Crop Health

Engineering Mycorrhizal Symbioses to Alter Plant Metabolism and Improve Crop Health

Mycorrhizal Root Exudates Induce Changes in the Growth and Fumonisin Gene (FUM1) Expression of Fusarium proliferatum

Editorial: Transport in Plant Microbe Interactions

Contact Info

Product

Resources

About