SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa

Pugliese, Nicola; Maimone, Francesco; Scrascia, Maria; Sf, Materu; Pazzani, Carlo

doi:10.1093/jac/dkn542

Cited by 29 publications

(31 citation statements)

References 8 publications

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“…Few previous surveys analyzed the prevalence of this family of ICEs, and they were limited to clinical isolates related to V. cholerae. Those studies reported the spread of ICE-harboring V. cholerae strains in Eastern Africa during the late 1990s (29) and also in India from 1994 to 2005 (10). Therefore, our results and previous data exhibited similar trends, indicating a gradual emergence of SXT/R391-like ICEs in bacteria of different environments and locations.…”

Section: Discussionsupporting

confidence: 88%

Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments

Rodríguez-Blanco

Lemos

Osorio

2012

Antimicrob Agents Chemother

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The presence of SXT/R391-related integrating conjugative elements (ICEs) in bacterial strains isolated from fish obtained from marine aquaculture environments in 2001 to 2010 in the northwestern Iberian Peninsula was studied. ICEs were detected in 12 strains taxonomically related to Vibrio scophthalmi (3 strains), Vibrio splendidus (5 strains), Vibrio alginolyticus (1 strain), Shewanella haliotis (1 strain), and Enterovibrio nigricans (2 strains), broadening the known host range able to harbor SXT/R391-like ICEs. Variable DNA regions, which confer element-specific properties to ICEs of this family, were characterized. One of the ICEs encoded antibiotic resistance functions in variable region III, consisting of a tetracycline resistance locus. Interestingly, hot spot 4 included genes providing resistance to rifampin (ICEVspPor2 and ICEValPor1) and quaternary ammonium compounds (QACs) (ICEEniSpa1), and variable region IV included a mercury resistance operon (ICEVspSpa1 and ICEEniSpa1). The S exclusion group was more represented than the R exclusion group, accounting for two-thirds of the total ICEs. Mating experiments allowed ICE mobilization to Escherichia coli strains, showing the corresponding transconjugants' rifampin, mercury, and QAC resistance. These results show the first evidence of ICEs providing rifampin and QAC resistances, suggesting that these mobile genetic elements contribute to the dissemination of antimicrobial, heavy metal, and QAC resistance determinants in aquaculture environments.

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Section: Discussionsupporting

confidence: 88%

Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments

Rodríguez-Blanco

Lemos

Osorio

2012

Antimicrob Agents Chemother

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“…the cephalosporin resistance gene bla CMY-2 16 and rifampicin resistance gene)13. In this study, isolates positive for resistant related genes at SXT/R391 ICEs were not always consistent with their phenotypic resistance (Table 1), this result indicated the phenotypic resistance may not be associated with genes encoded by ICEs, determinants of other mobile genetic elements, like plasmid36, transposon37 and genomic island38 within Enterobacteriaceae , are also mediated drug resistance. However, the role of ICEs in the acquisiton and transmission of antibiotic resistance should not be neglected, our study have displayed high transfer ability of ICEs from Proteus isolates to recipient cells.…”

Section: Discussionmentioning

confidence: 60%

SXT/R391 integrative and conjugative elements in Proteus species reveal abundant genetic diversity and multidrug resistance

et al. 2016

Sci Rep

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SXT/R391 integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are found in most members of Enterobacteriaceae. Here, we determined fifteen SXT/R391 ICEs carried by Proteus isolates from food (4.2%) and diarrhoea patients (17.3%). BLASTn searches against GenBank showed that the fifteen SXT/R391 ICEs were closely related to that from different Enterobacteriaceae species, including Proteus mirabilis. Using core gene phylogenetic analysis, the fifteen SXT/R391 ICEs were grouped into six distinct clusters, including a dominant cluster and three clusters that have not been previously reported in Proteus isolates. The SXT/R391 ICEs shared a common structure with a set of conserved genes, five hotspots and two variable regions, which contained more foreign genes, including drug-resistance genes. Notably, a class A β-lactamase gene was identified in nine SXT/R391 ICEs. Collectively, the ICE-carrying isolates carried resistance genes for 20 tested drugs. Six isolates were resistant to chloramphenicol, kanamycin, streptomycin, trimethoprim-sulfamethoxazole, sulfisoxazole and tetracycline, which are drug resistances commonly encoded by ICEs. Our results demonstrate abundant genetic diversity and multidrug resistance of the SXT/R391 ICEs carried by Proteus isolates, which may have significance for public health. It is therefore necessary to continuously monitor the antimicrobial resistance and related mobile elements among Proteus isolates.

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“…Kenyan isolates also lacked the dfrA18 gene (Kiiru et al, 2009). Since the emergence of SXT in V. cholerae O139, several studies on V. cholerae O1 have found this ICE as responsible for the dissemination of antibiotic resistance in Africa and Asia (Dalsgaard et al, 2001; Amita et al, 2003; Opintan et al, 2008; Adabi et al, 2009; Pugliese et al, 2009). ICEs of the SXT/R391 family are usually found in atypical O1 El Tor V. cholerae epidemic strains; they confer a narrow antibiotic resistance profile (Wozniak et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Superintegrons are the ancestors of multiresistant integrons (Mazel, 2006). Integrons and ICEs have been found in V. cholerae isolated in Mozambique, Iran, and India and they have largely contributed to the spread of antibiotic resistance (Amita et al, 2003; Adabi et al, 2009; Pugliese et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Identification of Atypical El TorV. cholerae O1 Ogawa Hosting SXT Element in Senegal, Africa

et al. 2017

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Vibrio cholerae O1 is the causative agent of cholera with classical and El Tor, two well-established biotypes. In last 20 years, hybrid strains of classical and El Tor and variant El Tor which carry classical ctxB have emerged worldwide. In 2004–2005, Senegal experienced major cholera epidemic with a number of cases totalling more than 31719 with approximately 458 fatal outcomes (CFR, 1.44%). In this retrospective study, fifty isolates out of a total of 403 V. cholerae biotype El Tor serovar Ogawa isolates from all areas in Senegal during the 2004–2005 cholera outbreak were randomly selected. Isolates were characterized using phenotypic and genotypic methods. The analysis of antibiotic resistance patterns revealed the predominance of the S-Su-TCY-Tsu phenotype (90% of isolates). The molecular characterization of antibiotic resistance revealed the presence of the SXT element, a self-transmissible chromosomally integrating element in all isolates. Most of V. cholerae isolates had an intact virulence cassette (86%) (ctx, zot, ace genes). All isolates tested gave amplification with primers for classical CT, and 10/50 (20%) of isolates carried classical and El Tor ctxB. The study reveals the presence of atypical V. cholerae O1 El Tor during cholera outbreak in Senegal in 2004–2005.

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SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa

Cited by 29 publications

References 8 publications

Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments

Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments

SXT/R391 integrative and conjugative elements in Proteus species reveal abundant genetic diversity and multidrug resistance

Identification of Atypical El TorV. cholerae O1 Ogawa Hosting SXT Element in Senegal, Africa

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