Switchable-hydrophilicity solvent liquid-liquid microextraction

Alshana, Usama; Hassan, Malek; Al‐Nidawi, Mais; Yılmaz, Erkan; Soylak, Mustafa

doi:10.1016/j.trac.2020.116025

Cited by 55 publications

(15 citation statements)

References 62 publications

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“…The pretreatment of biomass at less than pH 7 solubilized the hemicellulose fractions by adding HCl or H 2 SO 4 53 . Switchable hydrophilicity was reported for the extraction of metal ions and organic compounds by bubbling of CO 2 54 . An identical study was investigated using switchable solvent for the extraction of copper in food, water and hair samples with environmental applications 55 .…”

Section: Resultsmentioning

confidence: 99%

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Muniasamy

Balamurugan

Rajamahendran

et al. 2022

Sci Rep

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Fibrinolytic protease (FLP) is a therapeutic enzyme used in the treatment of thrombolytic diseases. The present study proposed the concept of pH-driven swappable micellar two-phase extraction for the concurrent production and purification of FLP from Bacillus subtilis at cloud point extraction. Extractive fermentation was carried out with a pH swap mechanism and FLP was extracted to the top phase by surfactant deep eutectic solvents (SDES). Shrimp waste was chosen as a sustainable low-cost substrate that yielded a maximum protease of 185 U/mg. Six SDESs were synthesized with nonionic surfactants as hydrogen bond donors and quaternary ammonium salts as hydrogen bond acceptors and their association was confirmed by H1 NMR. Thermophysical investigation of the synthetic SDES was accomplished as a function of temperature. Response surface methodology for extractive fermentation was performed with the concentration of SADES (35% w/v), Na2SO4 (15% w/v) and pH (6.3) as variables and the enzyme activity (248 IU/mg) as a response. Furthermore, purification using gel filtration chromatography was used to quantify the amount of enzyme obtained in the extraction phase (849 IU/ml). After final purification with an anion exchange column, the maximum purity fold (22.32) with enzyme activity (1172 IU/ml) was achieved. The in-vitro fibrinolytic activity has been confirmed using a fibrin plate assay.

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Section: Resultsmentioning

confidence: 99%

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Muniasamy

Balamurugan

Rajamahendran

et al. 2022

Sci Rep

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“…Recently, a new kind of LDS named switchable-hydrophilicity solvents (SHSs) [10] have been introduced in microextraction approaches. Although they have not been used exactly as extraction solvents in DLLME, their performance is similar to this approach in terms of dispersion.…”

Section: Switchable Hydrophilicity Solventsmentioning

confidence: 99%

“…In this sense, the employment of SHSs is based on the principles of the classical homogeneous liquid-liquid extraction (HLLE) [12], achieving an extremely large contact area and, therefore, improving the extraction efficiency. Moreover, this is achieved without the use of disperser organic solvents, avoiding the centrifugation step [10]. Despite all the above, SHSs have not attracted the interest compared with other alternative solvents used in liquid-phase microextraction techniques.…”

Section: Switchable Hydrophilicity Solventsmentioning

confidence: 99%

Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review

Grau

Azorín

Benedé

et al. 2021

J of Separation Science

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Dispersive liquid-liquid microextraction is one of the most widely used microextraction techniques currently in the Analytical Chemistry field, mainly due to its simplicity and rapidity. The operational mode of this approach has been constantly changing since its introduction, adapting to new trends and applications.Most of these changes are related to the nature of the solvent employed for the microextraction. From the classical halogenated solvents (e.g., chloroform or dichloromethane), different alternatives have been proposed in order to obtain safer and non-pollutants microextraction applications. In this sense, low density solvents, such as alkanols, switchable hydrophobicity solvents and ionic liquids were the first and most popular replacements for halogenated solvents, which provided similar or better results than these classical dispersive liquid-liquid microextraction solvents.However, despite the good performances obtained with low density solvents and

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“…Extraction, on the other hand, is often relegated to a supporting function, and it has only been in the last 15 years or so that the relevance of extraction technology has been acknowledged for its role in the development of highquality analytical data. [6][7][8][9] Extraction operations are often classified as "pre-treatment. " That is, extraction serves to separate analytes from potentially interfering reaction mixture while also preparing these analytes for assessment.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

Extraction Techniques used for the Removal of Pharmaceuticals from Environmental Samples

Soylak¹,

Jagirani²

2021

Pharm Sci

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Switchable-hydrophilicity solvent liquid-liquid microextraction

Cited by 55 publications

References 62 publications

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Use of green alternative solvents in dispersive liquid‐liquid microextraction: A review

Extraction Techniques used for the Removal of Pharmaceuticals from Environmental Samples

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