Swine Granulosa Cells Show Typical Endothelial Cell Characteristics

Basini, Giuseppina; Falasconi, Irene; Bussolati, Simona; Grolli, Stefano; Lecce, R. Di; Grasselli, Francesca

doi:10.1177/1933719115612130

Cited by 18 publications

(9 citation statements)

References 29 publications

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“…Previous data describes the SKI expression in the embryo during the development of blood vessels—especially the aorta, and suggests that this gene is involved in the regulation of TGF-β signalling in arterial media during embryonic development (Doyle et al 2012). This information justifies the recent finding of Basini et al, who presented the evidence that GCs cultured in endothelial culture medium (EBM-2) gained functional and phenotypic characteristics of endothelial cells (Basini et al 2016).…”

Section: Discussionsupporting

confidence: 86%

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

Kranc

Brązert

Budna

et al. 2018

Histochem Cell Biol

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The human ovarian granulosa cells (GCs) surround the oocyte and form the proper architecture of the ovarian follicle. The ability of GCs to proliferate and differentiate in the conditions of in vitro culture has been proven. However, there is still a large field for extensive investigation of molecular basics, as well as marker genes, responsible for these processes. This study aimed to find the new marker genes, encoding proteins that regulate human GCs in vitro capability for proliferation and differentiation during long-term primary culture. The human follicular GCs were collected from hyper-stimulated ovarian follicles during IVF procedures and transferred to a long-term in vitro culture. The culture lasted for 30 days, with RNA samples isolated at days 1, 7, 15, 30. Transcriptomic analysis was then performed with the use of Affymetrix microarray. Obtained results were then subjected to bioinformatical evaluation and sorting. After subjecting the datasets to KEGG analysis, three differentially expressed ontology groups “cell differentiation” (GO:0030154), “cell proliferation” (GO:0008283) and “cell–cell junction organization” (GO:0045216) were chosen for further investigation. All three of those ontology groups are involved in human GCs’ in vitro lifespan, proliferation potential, and survival capability. Changes in expression of genes of interest belonging to the chosen GOs were validated with the use of RT-qPCR. In this manuscript, we suggest that VCL, PARVA, FZD2, NCS1 , and COL5A1 may be recognized as new markers of GC in vitro differentiation, while KAT2B may be a new marker of their proliferation. Additionally, SKI, GLI2, FERMT2 , and CDH2 could also be involved in GC in vitro proliferation and differentiation processes. We demonstrated that, in long-term in vitro culture, GCs exhibit markers that suggest their ability to differentiate into different cells types. Therefore, the higher expression profile of these genes may also be associated with the induction of cellular differentiation processes that take place beyond the long-term primary in vitro culture. Electronic supplementary material The online version of this article (10.1007/s00418-018-1750-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 86%

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

Kranc

Brązert

Budna

et al. 2018

Histochem Cell Biol

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“…It is well known that a co-operation between these two types of cells under gonadotrophic stimulation is possible. Granulosa cells like endocrine cells are actively involved in follicle growth and development (Basini et al, 2016), whereas theca cells are needed for folliculogenesis to synthesize androgen and provide cross-talk with granulosa cells and oocyte (Canipari et al, 2012). In the present study, mRNA encoding AQP5 during the early luteal phase (days 2-4) was the lowest in both granulosa and theca cells, which corresponds with the stage of formation of primordial follicles.…”

Section: Discussionmentioning

confidence: 75%

“…The establishment and continual remodelling of a complex vascular system influence the follicle development. According to Basini et al (2016) this angiogenic process is modulated by the biosynthetic activity of granulosa cells. In the follicles of all stages, this layer of cells remains avascular up to ovulation (Binelli and Murphy, 2010).…”

Section: Discussionmentioning

confidence: 99%

The expression profile of AQP1, AQP5 and AQP9 in granulosa and theca cells of porcine ovarian follicles during oestrous cycle and early pregnancy

Młotkowska¹,

Tanski²,

Eliszewski³

et al. 2018

J. Anim. Feed Sci.

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“…CD105 null mice die by embryonic day 11.5 due to failure of endothelial remodeling [30], however, apart from playing an undoubtable role in vasculature development, endoglin is also involved in GCs function, since it is expressed by this type of cells [7,10,31]. It is generally accepted that follicle development is dependent on the establishment and continual remodeling of a complex vascular system, and was suggested that GCs may behave as endothelial cells and contribute to follicular angiogenesis [32]. Indeed, the study on porcine GCs conducted by Basini et al revealed that these cells express several endothelial markers, including CD105, and that this expression seems to be more pronounced in cells cultured in specific endothelial cell culture medium (EBM-2) [32].…”

Section: Discussionmentioning

confidence: 99%

“…It is generally accepted that follicle development is dependent on the establishment and continual remodeling of a complex vascular system, and was suggested that GCs may behave as endothelial cells and contribute to follicular angiogenesis [32]. Indeed, the study on porcine GCs conducted by Basini et al revealed that these cells express several endothelial markers, including CD105, and that this expression seems to be more pronounced in cells cultured in specific endothelial cell culture medium (EBM-2) [32]. We however observed gradual decrease of CD105 levels during porcine GCs in vitro culture, reaching the lowest level at 144 h, therefore it seems that in our culture conditions GCs lose their angiogenic potential.…”

Section: Discussionmentioning

confidence: 99%

Evidence for existence of molecular stemness markers in porcine ovarian follicular granulosa cells

Stefańska

Sibiak

Hutchings

et al. 2019

Medical Journal of Cell Biology

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Granulosa cells (GCs) are important component of the follicle, a principal functional unit of the ovary. They undergo highly dynamic changes during folliculogenesis and play a vital role in oocyte’s maturation. Recently, it has been shown that GCs also exhibit stem cell properties, since they express OCT-4, Nanog, Sox-2, which are markers of pluripotency, as well as several mesenchymal stem cell markers, such as CD29, CD44, CD90, CD105, CD117 or CD166. In addition, GCs are able to differentiate towards neurogenic, chondrogenic and osteogenic lineages. Since the use of embryonic stem cells in regenerative medicine is burdened with ethical concerns and the risk of immune rejection or teratoma formation, adult stem cells are emerging as a promising alternative. GCs especially seem to provide a promising source of stem cells, since they are easily obtainable during assisted reproduction techniques. In order to better understand the genetic changes taking place in proliferating granulosa cells cultured in vitro, we isolated GCs from 40 prepubertal gilts and cultured them in vitro for 168 h. After 24, 48, 72, 96, 120, 144 and 168 h of cultivation the total RNA was extracted, reverse transcription was conducted and RT-qPCR reaction was performed. We observed that CD44, CD90 and IGF1 were upregulated after the cultivation, whereas CD105 and LIF were downregulated. Collectively, our results confirm stemness potential of porcine GCs and provide an insight into the transcriptome changes during in vitro cultivation.Running title: Molecular stemness markers in porcine granulosa cells

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Swine Granulosa Cells Show Typical Endothelial Cell Characteristics

Cited by 18 publications

References 29 publications

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

The expression profile of <i>AQP1</i>, <i>AQP5</i> and <i>AQP9</i> in granulosa and theca cells of porcine ovarian follicles during oestrous cycle and early pregnancy

Evidence for existence of molecular stemness markers in porcine ovarian follicular granulosa cells

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