The expression profile of &lt;i&gt;AQP1&lt;/i&gt;, &lt;i&gt;AQP5&lt;/i&gt; and &lt;i&gt;AQP9&lt;/i&gt; in granulosa and theca cells of porcine ovarian follicles during oestrous cycle and early pregnancy

Młotkowska, Patrycja; Tanski, Damian; Eliszewski, Maciej; Skowrońska, Agnieszka; Nielsén, Sören; Skowroński, Mariusz T.

doi:10.22358/jafs/83596/2018

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…In most species of mammals, the oocyte matures within the ovarian follicle and during the ovulation period it is mainly in the stage of metaphase II (MII) meiosis. In light of studies on animals, it is assumed that an important role in these mechanisms can be played by connexins (Cxs) [32][33][34] and aquaporins (AQPs) [8,19,23,35]. Gap junctions appearing in oocyte-cumulus oophorus cell complexes are composed of protein Cxs [36].…”

Section: Discussionmentioning

confidence: 99%

Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

Tanski

Skowrońska

Eliszewski

et al. 2020

IJMS

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Aquaporins (AQPs) are a group of small, integral membrane proteins which play an important role in fluid homeostasis in the reproductive system. In our previous study, we demonstrated AQP1, 5 and 9 protein expression and localization in the porcine oviduct. The presence of these isoforms could suggest their role in the transport of the ovum to the uterus by influencing the epithelial cells’ production of oviductal fluid. The aim of this study was to evaluate the expression of AQP1, AQP5 and AQP9 in the infundibulum, ampulla and isthmus in the porcine oviduct during the estrous cycle (early luteal phase, days 2–4, medium luteal phase, days 10–12, late luteal phase days 14–16, follicular phase days 18–20) and pregnancy (period before implantation, days 14–16 and after the implantation, days 30–32) using the Real-Time PCR technique. As clearly demonstrated for the first time, AQP1, 5, and 9 gene expression is influenced by the estrus cycle and pregnancy. Furthermore, expression of AQPs in the porcine oviduct may provide the physiological medium that sustains and enhances fertilization and early cleavage-stage embryonic development. Overall, our study provides a characterization of oviduct AQPs, increasing our understanding of fluid homeostasis in the porcine oviduct to successfully establish and maintain pregnancy.

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Section: Discussionmentioning

confidence: 99%

Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

Tanski

Skowrońska

Eliszewski

et al. 2020

IJMS

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“…Furthermore, it was demonstrated that prolactin had a stimulating effect on AQP1 expression in the ovarian follicular cells. The authors suggest that AQP1 can also influence cell proliferation and migra-tion (44,60,(62)(63)(64). In addition, we recently analyzed the expression level of AQPs and Cxs genes in the pig's endometrium during primary in vitro culture (81).…”

Section: Role Of Aqps Gene Family In Reproductive Systems Processesmentioning

confidence: 99%

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

Kulus¹,

Wieczorkiewicz²,

Kulus³

et al. 2021

Medycyna Weterynaryjna

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The complexity of processes in the female reproductive system of mammals is extremely sophisticated. The overall relationship between the processes during the oestrus cycle in animals is quite well understood, but the molecular background of these processes still requires an in-depth analysis. Bitches are distinguished by a specific course of sexual cycle during which the oocyte matures after ovulation in the oviduct. Other species of mammals are characterized by maturation of the oocyte within the ovary. Acquisition of developmental competence by cumulus – oocyte complexes seems to be a process with a complex molecular background, and the key to understanding it may be the analysis of intercellular channels. Aquaporins and connexins are structural proteins that are built into the cell membrane. Their location is widespread in many body tissues. Recent years have shown that they exhibit significant expression in different parts of the mammalian reproductive system, although the number of studies on dogs is still negligible. This review paper presents the current state of knowledge of water channels and gap junction connections in different animal species, with particular focus on dogs, and also explores the role of aquaporins and connexins in the acquisition of reproductive competences.

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The expression profile of <i>AQP1</i>, <i>AQP5</i> and <i>AQP9</i> in granulosa and theca cells of porcine ovarian follicles during oestrous cycle and early pregnancy

Cited by 2 publications

References 32 publications

Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

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