Swine as a Potential Reservoir of Shiga Toxin-ProducingEscherichiacoliO157:H7 in Japan

Nakazawa, Muneo; Akiba, Masato

doi:10.3201/eid0506.990618

Cited by 63 publications

(40 citation statements)

References 8 publications

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“…All human and bovine strains as well as 11 isolates recovered from swine, were classified as STEC, producing either Stx1, Stx2 or both toxins. Strains O157:H7 + or O157:H -have been o�en isolated from ca�le but there is li�le information concerning the prevalence and characteristic of this bacteria recovered from pigs (Beutin et al, 1993;Wray et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999;Nakazawa et al, 1999). Wray et al (1993) and Wi�ig et al (1995) described a few O157 E. coli strains of swine origin and found that they were different from other O157 isolates in terms of Shiga toxin production (usually Stx-negative).…”

Section: Antimicrobialsmentioning

confidence: 99%

“…There is li�le information concerning the prevalence and characteristics of E. coli O157 originating from pigs and pork products are not regarded as a risk factor for human STEC infection (Beutin et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999). However, studies from Chile and Japan have documented a carriage rate of E. coli O157 in pigs comparable to the carriage rate in ca�le (Nakazawa et al, 1999;Rios et al, 1999).…”

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confidence: 99%

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as for antimicrobial resistance and biochemical properties. Fourteen of the human strains (n = 23) and 21 of the bovine isolates (n = 29) were of the O157:H7 serotype as determined by agglutination and PCR methods. All E. coli O157 of porcine origin (n = 38) were H-negative. Based on the ability to produce Shiga toxins (Stxs), all human and cattle isolates and 11 strains recovered from swine were identified as Shiga toxin-producing E. coli (STEC). Among STEC, most human strains (18 isolates) were Stx1- and Stx2-positive whereas cattle strains were mostly Stx2-positive. Eleven porcine STEC produced either Stx1 (7 isolates) or Stx2 (4 strains) toxins; an additional 20 isolates recovered from these animals had the Stx2e toxin gene as previously determined by PCR. All human and cattle E. coli O157 produced enterohemolysin whereas only 4 strains recovered from pigs were ehly-positive. Moreover, the PCR identification of the lpfO113 gene performed earlier revealed that this putative virulence marker was present in all porcine isolates, only in 5 strains of bovine origin but in none of E. coli O157 recovered from humans. All 90 E. coli O157 strains tested displayed 10 biochemical profiles that were different at least in one of the reaction tested. The most common atypical reaction observed among porcine O157 isolates was ability to ferment sorbitol (all strains) and production of β-glucuronidase (25 isolates). Moreover, none of the sorbitol-positive strains was able to produce indol. Four antimicrobial resistance profiles among 90 E. coli O157 strains tested were observed. Most of the isolates recovered from humans and all strains from cattle were resistant only to rifampicin whereas the porcine strains showed resistance to either 3 antimicrobials (4 isolates) or to 4 drugs tested (34 isolates). The phenotypic data shown in the present study, together with the previously published genotypic analyses of these strains, confirm earlier suggestions that the porcine E. coli O157 strains are mostly different from those of bovine and human O157 isolates and could therefore play less important role in human STEC O157 infections.

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Section: Antimicrobialsmentioning

confidence: 99%

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confidence: 99%

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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“…Cattle have been implicated as a major reservoir of E. coli O157 and there are only few reports describing the isolation and characterisation of this bacteria from pigs (Beutin et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999;Nakazawa et al, 1999). In the present study, five isolates of pig origin were analysed and compared with nine O157 strains isolated from cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Most of these isolates were recovered from cattle and from children with diarrhoea. Moreover, there is only one report describing the prevalence of E. coli O157 of pig origin (Osek, 2002) and there are few papers about such strains from other countries (Beutin et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999;Nakazawa et al, 1999). In the present study, twelve Polish isolates (seven of bovine origin and five recovered from pigs, respectively) and two German strains (both isolated from cattle) were tested for STEC virulence marker genes and for their clonal relationship using PCR and PFGE methods, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…STEC of the O157 serogroup have also been isolated from sheep, goats, dogs, poultry, and seagulls Mainil, 1999). However, there is little information concerning the prevalence and characteristics of these bacteria originating from pigs (Beutin et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999;Nakazawa et al, 1999). Moreover, the E. coli O157 recovered from pigs were often different from other animal and human STEC in the absence of stx and accessory virulence marker genes (Wray et al, 1993;Wittig et al, 1995).…”

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Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods

Osek¹,

Gallien²

2002

Vet. Med. - Czech

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ABSTRACT:Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC). Only two strains, both of cattle origin, were positive for the fliC (H7) gene and could be classified as O157 : H7. Nine isolates had stx shiga toxin genes, either stx1 (1 strain), stx2 (4 isolates) or both (4 strains). e stx2-carrying samples were further subtyped by PCR for the stx2c, stx2d, and stx2e toxin variants. It was shown that all but one stx2-positive bacteria possessed the stx2c Shiga toxin gene type and one stx2 STEC isolate had the stx2d virulence factor subtype. e eaeA (intimin) gene was found in 9 strains (8 isolates from cattle and one strain from pigs); all of them harboured the genetic marker characteristic of the gamma intimin variant. e translocated intimin receptor (tir) gene was detected in 7 isolates tested and among them only one tir-positive strain was recovered from pigs. e ehly E. coli enterohemolysin gene was amplified in all but one strains obtained from cattle and only in one isolate of porcine origin. e genetic relatedness of the analysed E. coli O157 strains was examined by restriction fragment length polymorphism (RFLP) of chromosomal DNA digested with XbaI. Two distinct but related RFLP pattern clusters were observed: one with 9 strains (8 isolates of bovine origin and one strain obtained from pigs) and the other one comprises the remaining 5 E. coli isolates (4 of porcine origin and one strain recovered from cattle). e results suggest that pigs, besides cattle, may be a reservoir of E. coli O157 strains potentially pathogenic to humans. Moreover, epidemiologically unrelated isolates of the O157 serogroup, recovered from different animal species, showed a clonal relationship as demonstrated by the RFLP analysis.

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Epidemiology of VTEC in Non‐Ruminant Animals

Wasteson

2004

Verocytotoxigenic E. Coli

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Swine as a Potential Reservoir of Shiga Toxin-ProducingEscherichiacoliO157:H7 in Japan

Cited by 63 publications

References 8 publications

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods

Epidemiology of VTEC in Non‐Ruminant Animals

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