Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek, Jacek

doi:10.17221/5711-vetmed

Cited by 10 publications

(10 citation statements)

References 39 publications

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“…Similar results for predominance of stx2 producing strains were reported by Wani et al, (2003); Blanco et al, (2004);Osek (2004); Hazarika et al, (2007);Milnes et al, (2008);and Salnec et al, (2009). In this study lower prevelance of stx1 is reported which is similar to finding reported by Galland et al, (2001); Blanco et al, (2004);Osek (2004); Arya et al, (2008) and Milnes et al, (2008).…”

Section: Resultssupporting

confidence: 91%

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Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples

Sinha

Kshirsagar

Brahmbhatt

et al. 2015

IJAR

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Shiga-toxigenic Escherichia coli (STEC) are causative agents of bloody diarrhoea, haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). Humans acquire infections primarily through contaminated beef. In India, STEC has not been implicated as a major cause of diarrhoea. The study was carried out to know the prevalence of shiga toxin (ST) producing Escherichia coli (STEC) and virulence genes characterization from buffalo meat samples sold at various retail meat shops at Anand, India. In the present investigation 70 isolates of Escherichia coli isolated from 150 retail market buffalo meat samples, were screened for STEC, using conventional culture methods, serotyping and polymerase chain reaction (PCR). Out of 70 E. coli isolates 11 different 'O' serogroups were recorded in 44 isolates. While 19 isolates were untypable and seven were rough isolates. Out of the 70 E. coli tested 56 isolates (80%) were positive for stx genes: of which 51 (72.85%) harboured stx2 genes and 2 isolates (2.85%) were positive for stx1 gene only. Moreover, 3 E. coli isolates (4.28%) harboured both stx1 and stx2. About 66 (94.28%) isolates were positive for eaeA gene. While out of 70 E. coli isolates tested, 3 (4.28%) were found to be positive for rfb O157 gene. Presence of STEC and other virulence factors in buffalo meat samples appeared to be matter of concern and threat to public health.

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Section: Resultssupporting

confidence: 91%

“…However, lesser isolation rate for stx positive E. coli isolates has been reported by Rahman (2002) and Chattopadhyay et al, (2003). Detailed result is given in Galland et al, (2001); Wani et al, (2003) and Osek (2004) which is in contrast to lower prevalence as reported by Blanco et al, (2004); Mora et al, (2007) and Arya et al, (2008).…”

Section: Resultsmentioning

confidence: 51%

Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples

Sinha

Kshirsagar

Brahmbhatt

et al. 2015

IJAR

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“…In PCR analysis of their results, 26.0% (25/96) of the non‐sorbitol positive E. coli O157:H7 + and all of the E. coli O157:H7 ‐ isolates harbored one or more antibiotic resistance genes, while tetA, tetB, tetC, strA, strB and sulI genes were detected from a number of the isolates, tetD, tetE, tetG, cmlA, floR, sulII, aadA and ampC genes were not detected in any of the isolates. Osek () reported similar results. He showed that phenotypic characterization of antibiotic resistance in E. coli strains can further approved by the genotypic identification.…”

Section: Discussionsupporting

confidence: 57%

Shiga (vero) toxin producing Escherichia coli in various types of food stuffs; virulence factors, O‐serogroups and antimicrobial resistance properties

Ranjbar

Sheikhshahrokh

Jafari

2016

Journal of Food Safety

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The aim of the present study was to determine the incidence of Shiga toxin producing Escherichia coli, its virulence factors, O-serogroups and antimicrobial resistance properties in various types of food stuffs in Iran. All samples were cultured and those that were E. coli positive were subjected to PCR. Of 801 samples studied, 123 (15.35%) were positive for STEC strains. Samples collected in summer season had the higher prevalence of bacteria (49.59%). Beef meat was the most commonly contaminated sample (24.28%). The stx1 was the most commonly detected, while ehly was the least. The most commonly detected serogroups in the STEC strains recovered from the food samples were O157 (27.64%) and O26 (21.95%). The serogroup of 17.07% of STEC strains cannot be detected by means of the O-serogroup primers. The most commonly detected antibiotic resistance genes were blaSHV (47.15%) and aadA1 (44.71%). STEC strains of our investigation harbored the highest levels of resistance against ampicillin (100%), gentamycin (70.73%) and tetracycline (62.60%). Observation of proper monitoring, principles of food hygiene and accurate antibiotic prescription reduce the risk of diseases caused by STEC strains in human. Practical applicationsWe found E. coli bacteria with defined pathotypes which originates mainly from human and animal sources in various types of food samples of Iranian markets. We also identified a large numbers of virulent and resistant strains of E. coli with higher prevalence of stx1 and eaeA gene, O157 and O26 serogroups, CITM and aac(3)-IV antibiotic resistance genes and resistance to ampicillin, penicillin and gentamycin antibiotics. Beef meat had the highest prevalence. Simultaneous presence of stx1 and eaeA and stx2 and eaeA virulence factors in some strains of E. coli bacterium in food stuffs indicated the important public health problem. Using from microbiological tests can contribute to reducing the microbial load in food stuffs. Controlling sources of human and animal fecal pollution, such as managing the quality of primary ingredients, staffs and devices are other approaches to reduce the prevalence of STEC in food stuffs. Using from the disk diffusion method and avoiding from the irregular and excessive prescription of antibiotics are two important approaches to reduce the levels of antibiotic resistance. K E Y W O R D S antimicrobial resistance properties, food stuffs, Iran, O-serogroups, Shiga toxin producing Escherichia coli, virulence factors

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“…It was continued by 35 cycles, each cycle including denaturation 60 seconds at 94°C, annealing 30 seconds at 53°C, and extension 60 seconds at 72°C. Final extention was done 7 minutes at 72°C (Osek, 2004;Pradel et al 2000). from puppies and adult dogs with diarrhea at a percentage (68.75%) and from puppies and adults without diarrhea at a percentage (7.95%).…”

Section: C-thermo-cycler Programmentioning

confidence: 99%

Detection of Virulent Genes in E. coli O157:H7 Isolated from Puppies and Adult Dogs by Polymerase Chain Reaction

Hasan¹,

Yousif²,

Alwan³

2016

Res. J. Vet. Pract.

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| E. coli O157:H7 is considered as an important pathogen of diarrhea in adult dogs and puppies. The presented study aimed to isolate E. coli O157:H7 serotypes from dogs and puppies and to detection of the stx1, stx2 and eaeA virulence genes in these strains using conventional PCR assay. One hundred and four fecal swab samples were collected from dogs and puppies with different ages, breeds and sex. After primary cultivation of the collected samples onto MacConkey's and EMB agar media, the suspected E. coli colonies were subcultured onto CHROM and CT-SMAC agar media. The non sorbitol fermenter E. coli were serotyped using latex agglutination test for detection of O157 and H7 antigens. The results of latex test revealed 18 E. coli O157:H7 isolates. From 16 diarrheic animals, 11 (68.75%) were positive for E. coli O157:H7; 8 (72.72%) from puppies and 3 (60%) from adults. Meanwhile, 7 E. coli O157:H7 isolates were recovered from 88 non diarrheic animals (7.95 %); 4 (9.52%) from puppies and 3 (6.52%) from adults. The 18 isolates of E. coli O157:H7 were conducted to PCR assay, the results showed that 6 (33.33%) and 1(5.55%) E. coli isolates had stx1 and eaeA genes, respectively. The results also showed that three isolates (16.66%) had all the investigated genes, three isolates (16.66%) had Stx1 and eaeA genes, one isolate (5.55%) had Stx2 and eaeA genes, while four (22.22%) isolates were negative for these virulence genes. In conclusion, we may suggest that dogs and puppies can be regarded as important reservoirs for E. coli O157:H7 which is one of the main causes of diarrhea and other diseases in human.

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Cited by 10 publications

References 39 publications

Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples

Detection and virulence gene characterization of Shiga toxigenic E. coli from buffalo meat samples

Shiga (vero) toxin producing Escherichia coli in various types of food stuffs; virulence factors, O‐serogroups and antimicrobial resistance properties

Detection of Virulent Genes in E. coli O157:H7 Isolated from Puppies and Adult Dogs by Polymerase Chain Reaction

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