Sweetpotato vines hydrolysate promotes single cell oils production of Trichosporon fermentans in high-density molasses fermentation

Shen, Qi; Lin, Hui; Wang, Qun; Fan, Xiaoping; Yang, Yuyi; Zhao, Yanfang

doi:10.1016/j.biortech.2014.11.045

Cited by 28 publications

(12 citation statements)

References 34 publications

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“…However, T. fermentans CICC 1368 lipids produced on molasses-based media contained stearic (C18:0) and linolenic acids (C18:3). Oleic acid (C18:1) was only be detected at low levels in sugar-based media and was not detected after incubation with glycerol (Shen et al 2015). Three major fatty acids of the same strain of oils obtained from medium with fructose were palmitic acid (16:0), stearic acid (18:0), and oleic acid (18:1), and these three fatty acids accounted for over 95% of the total fatty acids (Bao et al 2018).…”

Section: Biomass Yield and Lipid Contentmentioning

confidence: 98%

“…The final content of lipids in T. domesticum PCM 2960 biomass after bioreactor incubation with DPW with glucose was 33% CDW and as a consequence lipid yield exceeded 4.8 g L −1 . T. fermentans CICC 1368 showed low lipid accumulation on pure molasses, but enzymatic hydrolysate supplementation of sweet potato vines increased lipid content (Shen et al 2015). Inulin can be directly utilized by T. cutaneum for microbial lipid fermentation without a hydrolysis step and 4.79 g/L of lipid has been produced from 50 g/L inulin (Wang et al 2015).…”

Section: Biomass Yield and Lipid Contentmentioning

confidence: 99%

“…The enzyme has an optimum pH in the range of 8.0-8.5 and an optimum temperature of 32°C and shows no metal dependence on the chloride salts of sodium, potassium, magnesium, or ferrum (Goldson-Barnaby and Scaman 2013). Trichosporon fermentans can produce lipids with various cheap substrates such as rice straw hydrolysates (Huang et al 2009) and molasses (Zhu et al 2008;Shen et al 2015) or nitrogen-rich Jerusalem artichoke hydrolysates (Bao et al 2018). T. cutaneum produces biomass rich in oils after incubation on steam-exploded and three-stage enzymatic hydrolysis corn stover.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Gientka

Aleksandrzak‐Piekarczyk

Bzducha-Wróbel

et al. 2019

Potato Res.

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This study determines the ability of an isolated Trichosporon domesticum yeast strain to accumulate intracellular lipids in media with deproteinated potato wastewater (DPW) containing various carbon sources. The yeast strain was isolated from kefir and identified by internal transcribed spacer (ITS) region sequence analysis. The sequence was deposited in GenBank under accession number MH094668, and the strain was deposited in Polish Collection of Microorganisms as T. domesticum PCM 2960. DPW is an inexpensive and valuable source of nitrogen, potassium, phosphorus, and other elements in yeast cultures. DPW supplemented with glucose medium was most effective at stimulating lipid biosynthesis by T. domesticum PCM 2960 and bioreactor incubation resulted in a final lipid yield of 4.8 g L −1. The lipids of the T. domesticum PCM 2960 biomass were characterized by high contents of linoleic acid (Δ9,12 C18:2), oleic acid (Δ9 C18:1), palmitic acid (C16:0), and α-linolenic acid (Δ9,12,15 C18:3). Theoretical calculations for biodiesel properties showed that the methylated esters of lipids from T. domesticum PCM 2960 biomass cannot be used as a biodiesel in diesel engines. Additionally, the ability to produce biofilm as one criterion for pathogenicity was tested. The ability for biofilm formation by the isolated strain was low. This study provides a promising solution for the more economical production of microbial lipids with DPW.

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Section: Biomass Yield and Lipid Contentmentioning

confidence: 98%

Section: Biomass Yield and Lipid Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Gientka

Aleksandrzak‐Piekarczyk

Bzducha-Wróbel

et al. 2019

Potato Res.

View full text Add to dashboard Cite

show abstract

“…primarily sucrose, glucose, and fructose), and is abundant, low-cost and highly available for the fermentation industry [5][6][7]. Thereby, molasses is widely used for microbial conversion into different value-added products, such as bioethanol, baker's yeast, amino acids, butanol, organic acids, single cell proteins, etc [8][9][10][11]. However, the fermentation poses a severe waste disposal problem worldwide [12], because most wastewater is discharged into the environment without appropriate pre-treatment, aggravating the dire water pollution situations, and contributing to severe environmental pollution [1,13,14].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Li¹,

Wang²,

Ke³

et al. 2020

Preprint

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Background: Molasses is a wildly used feedstock for fermentation, but it poses a severe wastewater-disposal problem worldwide. Recently, the wastewater produced by yeast during molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied in soil. In this study, the low-cost FA powder was bioconverted into a value-added product, γ-PGA, by a glutamate independent producer, Bacillus velezensis GJ11. Results: With FA powder, the substrates of sodium glutamate and citrate sodium used in medium were decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium. In the optimized FA powder fermentation medium, the γ-PGA was produced with its maximum concentration at 42.55 g/L and a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolyzates was dependent on the ethylene signalling and NPR1. Conclusions: To our knowledge, this is the first report of using the industry waste, FA powder, as a sustainable substrate for the microbial synthesis of γ-PGA. This bioprocess can not only develop a new way of FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

show abstract

“…primarily sucrose, glucose, and fructose), and is abundant, low-cost and highly available for the fermentation industry [5][6][7]. Thereby, molasses is widely used for microbial conversion of different value-added products, such as bioethanol, baker's yeast, amino acids, butanol, organic acids, single cell proteins, etc [8][9][10][11]. However, the fermentation also causes a severe waste disposal problem worldwide [12], because most wastewater is discharged into the environment without appropriate pre-treatment, aggravating the dire water pollution situations [1,13,14].…”

Section: Introductionmentioning

confidence: 99%

Microbial synthesis of poly- γ -glutamic acid ( γ -PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Wang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate independent producer of Bacillus velezensis GJ11.Results: FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signalling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions: To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

show abstract

Sweetpotato vines hydrolysate promotes single cell oils production of Trichosporon fermentans in high-density molasses fermentation

Cited by 28 publications

References 34 publications

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Microbial synthesis of poly- γ -glutamic acid ( γ -PGA) with fulvic acid powder, the waste from yeast molasses fermentation

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