Abstract:This chapter focuses on the breeding of new sweet cherry cultivars. Information on the origin, parentage, tree growth, productivity, phenology, fruit characteristics and resistance to various biotic and abiotic factors of these sweet cherry cultivars are also presented.
“…'Ambrunés' is a vigorous, self-incompatible, early flowering and very late ripening (+31 days after 'Burlat') cultivar. The fruits are heart-shaped, of medium size, garnet skin colour with orange flesh, harvested without the peduncle and exhibits high resistance to fruit cracking (Gella et al 2001;Quero-García et al 2017). Also, fruit firmness is well maintained during ripening providing outstanding post-harvest quality (Serradilla et al 2010).…”
The Spanish local cultivar 'Ambrunés' stands out due to its high organoleptic quality and fruit firmness. These characteristics make it an important parent for breeding cherries with excellent fresh and post-harvest quality. In this work, an F1 sweet cherry population (n=140) from 'Ambrunés' × 'Sweetheart' was phenotyped for two years for fruit diameter, weight and firmness and genotyped with the RosBREED cherry Illumina Infinium ® 6K SNP array v1. These data were used to construct a linkage map and to carry out QTL mapping of these fruit quality traits. Genotyping of the parental cultivars revealed that 'Ambrunés' is highly heterozygous, and its genetic map is the longest reported in the species using the same SNP array. Phenotypic data analyses confirmed a high heritability of fruit size and firmness and a distorted segregation towards softer and smaller fruits. However, individuals with larger and firmer fruits than the parental cultivars were observed, revealing the presence of alleles of breeding interest. In contrast to other genetic backgrounds in which a negative correlation was observed between firmness and size, in this work, no correlation or low positive correlation was detected between both traits. Firmness, diameter and weight QTLs detected validated QTLs previously found for the same traits in the species and major QTLs for the three traits were located on a narrow region of LG1 of 'Ambrunés'.Haplotype analyses of these QTLs revealed haplotypes of breeding interest in coupling phase in 'Ambrunés', which can be used for the selection of progeny with larger and firmer fruits.
“…'Ambrunés' is a vigorous, self-incompatible, early flowering and very late ripening (+31 days after 'Burlat') cultivar. The fruits are heart-shaped, of medium size, garnet skin colour with orange flesh, harvested without the peduncle and exhibits high resistance to fruit cracking (Gella et al 2001;Quero-García et al 2017). Also, fruit firmness is well maintained during ripening providing outstanding post-harvest quality (Serradilla et al 2010).…”
The Spanish local cultivar 'Ambrunés' stands out due to its high organoleptic quality and fruit firmness. These characteristics make it an important parent for breeding cherries with excellent fresh and post-harvest quality. In this work, an F1 sweet cherry population (n=140) from 'Ambrunés' × 'Sweetheart' was phenotyped for two years for fruit diameter, weight and firmness and genotyped with the RosBREED cherry Illumina Infinium ® 6K SNP array v1. These data were used to construct a linkage map and to carry out QTL mapping of these fruit quality traits. Genotyping of the parental cultivars revealed that 'Ambrunés' is highly heterozygous, and its genetic map is the longest reported in the species using the same SNP array. Phenotypic data analyses confirmed a high heritability of fruit size and firmness and a distorted segregation towards softer and smaller fruits. However, individuals with larger and firmer fruits than the parental cultivars were observed, revealing the presence of alleles of breeding interest. In contrast to other genetic backgrounds in which a negative correlation was observed between firmness and size, in this work, no correlation or low positive correlation was detected between both traits. Firmness, diameter and weight QTLs detected validated QTLs previously found for the same traits in the species and major QTLs for the three traits were located on a narrow region of LG1 of 'Ambrunés'.Haplotype analyses of these QTLs revealed haplotypes of breeding interest in coupling phase in 'Ambrunés', which can be used for the selection of progeny with larger and firmer fruits.
Dormancy release and bloom time of sweet cherry cultivars depend on the environment and the genotype. The knowledge of these traits is essential for cultivar adaptation to different growing areas, and to ensure fruit set in the current climate change scenario. In this work, the major sweet cherry bloom time QTL qP-BT1.1m (327 Kbs; Chromosome 1) was scanned for candidate genes in the Regina cv genome. Six MADS-box genes (PavDAMs), orthologs to peach and Japanese apricot DAMs, were identified as candidate genes for bloom time regulation. The complete curated genomic structure annotation of these genes is reported. To characterize PavDAMs intra-specific variation, genome sequences of cultivars with contrasting chilling requirements and bloom times (N = 13), were then mapped to the ‘Regina’ genome. A high protein sequence conservation (98.8–100%) was observed. A higher amino acid variability and several structural mutations were identified in the low-chilling and extra-early blooming cv Cristobalina. Specifically, a large deletion (694 bp) upstream of PavDAM1, and various INDELs and SNPs in contiguous PavDAM4 and -5 UTRs were identified. PavDAM1 upstream deletion in ‘Cristobalina’ revealed the absence of several cis-acting motifs, potentially involved in PavDAMs expression. Also, due to this deletion, a non-coding gene expressed in late-blooming ‘Regina’ seems truncated in ‘Cristobalina’. Additionally, PavDAM4 and -5 UTRs mutations revealed different splicing variants between ‘Regina’ and ‘Cristobalina’ PavDAM5. The results indicate that the regulation of PavDAMs expression and post-transcriptional regulation in ‘Cristobalina’ may be altered due to structural mutations in regulatory regions. Previous transcriptomic studies show differential expression of PavDAM genes during dormancy in this cultivar. The results indicate that ‘Cristobalina’ show significant amino acid differences, and structural mutations in PavDAMs, that correlate with low-chilling and early blooming, but the direct implication of these mutations remains to be determined. To complete the work, PCR markers designed for the detection of ‘Cristobalina’ structural mutations in PavDAMs, were validated in an F2 population and a set of cultivars. These PCR markers are useful for marker-assisted selection of early blooming seedlings, and probably low-chilling, from ‘Cristobalina’, which is a unique breeding source for these traits.
“…Denemede değerlendirilen çeşitlerin birbirleri ile ve serbest tozlanarak oluşturdukları meyve tutma oranları 2017 yılı üretim sezonu için tespit edilmiştir. [13].…”
Özet: Erkenci bazı kiraz çeşitlerinin birbirlerine tozlayıcı çeşit olabilme özellikleri incelenmiştir. Bu amaçla, Early Lory, Cristalina ve Prime Giant çeşitlerinin çiçek tozları sayılmış, çimlendirme ve canlılık testleri yapılarak, nihai meyve tutumları belirlenmiştir. Early Lory ve Cristalina daha fazla sayıda çiçek tozuna sahip çeşitler olarak belirlenmiştir. Prime Giant bu çeşitler arasında düşük çiçek tozu sayısı oluşturarak dikkat çekmektedir. Ancak, çiçek tozu miktarının aksine en yüksek çiçek tozu canlılık oranının Prime Giant (%88.87) çeşidinde olduğu saptanmıştır. Cristalina (%69.04) ve Early Lory (%51.55) çeşitleri daha az sayıda canlı çiçek tozuna sahip olmuşlardır. Çiçek tozu çimlenme düzeyleri %7.62 ile %22.82 arasında değişmiş olup, çeşitler arasında önemli farklılık bulunmamıştır. En yüksek çiçek tozu çimlenme oranları %15 sakkaroz dozu içeren ortamda elde edilmiştir. Sonuç olarak Early Lory çeşidi için hem Cristalina, hem de Prime Giant çeşitlerinin tozlayıcı olarak kullanılabileceği; buna karşın, Cristalina ve Prime Giant çeşitleri ile bahçe tesis edilecek ise her iki çeşidin de farklı tozlayıcılar istediği dikkate alınmalıdır.
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