Fruit size and firmness QTL alleles of breeding interest identified in a sweet cherry ‘Ambrunés’ × ‘Sweetheart’ population

Calle, Alejandro; Balas, F.; Cai, Lichun; Iezzoni, Amy; López-Corrales, M.; Serradilla, Manuel Joaquín; Wünsch, Ana

doi:10.1007/s11032-020-01165-1

Cited by 18 publications

(21 citation statements)

References 67 publications

(108 reference statements)

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“…Several QTLs and candidate genes linked to fruit quality traits in Prunus have been described in other species ( Aranzana et al, 2019 ), such as peaches ( Dirlewanger et al, 2004 ; Quilot et al, 2005 ) and cherries ( Wang et al, 2000 ). Phenotypic data for two consecutive years confirmed a high heritability of fruit size, FF, and FW QTLs were located on a narrow region of LG1 of Ambrunés cherry ( Calle et al, 2020 ). A major QTL for FF, named qP-FF4.1 , was identified in three sweet cherry populations ( Cai et al, 2019 ), and candidate genes related to plant cell wall modification and various plant hormone signaling pathways were identified, with an expansin gene being the most promising candidate.…”

Section: Introductionmentioning

confidence: 71%

Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci Linked to Fruit Quality Traits in Apricots Using Specific-Locus Amplified Fragment Sequencing

Zhang

Liu

et al. 2022

Front. Plant Sci.

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Improving fruit quality is one of the main tasks in modern commercial apricot breeding. Because of the lack of high-density linkage maps and fine mapping, it is difficult to obtain molecular markers that can assist in breeding for quantitative inheritance of fruit quality traits. In this study, specific-locus amplified fragment sequencing was used to genotype 169 seedlings of F1 apricot (Prunus armeniaca L.) progenies derived from crossing “Chuanzhihong” (H) with “Saimaiti” (S). After aligning to the Prunus armeniaca reference genome and filtering out low-quality variants, 6,012 high-quality single nucleotide polymorphisms were obtained and employed to construct a genetic map for each parent. The genetic linkage maps showed eight linkage groups of apricot, covering a distance of 809.6 cM in “H” and 1076.4 cM in “S”. The average distance between markers in “H” and “S” was 0.62 and 0.95 cM, respectively. To map quantitative trait loci (QTLs) for fruit quality, we investigated fruit quality traits, including fruit weight (FW), fruit height (FH), fruit lateral width (FL), fruit ventral width (FV), soluble solids content (SSC), and fruit firmness (FF) for all seedlings genotyped in 2018 and 2019. Eleven and nine QTLs linked to fruit quality traits were anchored on the “H” and “S” maps, respectively, and 1,138 putative candidate genes for 16 most significant regions on the corresponding chromosome were identified based on gene annotation. Among them, fruit size contained 648 genes in 11 intervals on the reference genome, SSC contained 372 genes in 3 intervals, and FF contained 117 genes in 2 intervals. Our findings uncovered the genetic basis of apricot fruit quality, and provided candidate genes for further molecular genetic studies on fruit quality and QTL targets for future marker-assisted selection of apricot quality improvement breeding.

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Section: Introductionmentioning

confidence: 71%

Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci Linked to Fruit Quality Traits in Apricots Using Specific-Locus Amplified Fragment Sequencing

Zhang

Liu

et al. 2022

Front. Plant Sci.

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“…On the other hand, regarding the identification of candidate genes to MAS development, in peaches, several major genes have been cloned or fine mapped and are postulated as strong candidate genes applied to MAS linked to fruit quality traits [ 224 ]: NAC TF for ripening date in peaches [ 225 ], cell number regulator (CNR) for fruit size in peaches and cherries [ 226 , 227 ], leucine - rich repeat receptor - like kinase for fruit shape [ 228 ], cleavage carotenoid dioxygenase (CCD) for flesh color (white/yellow) [ 95 , 229 ], MYB TFs for flesh and skin color in peaches [ 230 , 231 ], NAC TFs for blood flesh [ 207 ], auxin efflux carrier family for fruit acidity [ 232 ], endopolygalacturonase for melting flesh and clingstone in peaches [ 233 , 234 ], yucca (YUC) protein for stony hard texture [ 202 ], and R2R3 MYB transcription factor for glabrous skin in peaches [ 235 ]. Other key genes and regulators of slow ripening [ 236 ], volatile compounds [ 237 ], and anthocyanin accumulation in the skin [ 210 ] have also been identified in peaches.…”

Section: Application Of New Molecular Tools To Prunus mentioning

confidence: 99%

Molecular Bases of Fruit Quality in Prunus Species: An Integrated Genomic, Transcriptomic, and Metabolic Review with a Breeding Perspective

García-Gómez

Salazar

Nicolás-Almansa

et al. 2020

IJMS

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In plants, fruit ripening is a coordinated developmental process that requires the change in expression of hundreds to thousands of genes to modify many biochemical and physiological signal cascades such as carbohydrate and organic acid metabolism, cell wall restructuring, ethylene production, stress response, and organoleptic compound formation. In Prunus species (including peaches, apricots, plums, and cherries), fruit ripening leads to the breakdown of complex carbohydrates into sugars, fruit firmness reductions (softening by cell wall degradation and cuticle properties alteration), color changes (loss of green color by chlorophylls degradation and increase in non-photosynthetic pigments like anthocyanins and carotenoids), acidity decreases, and aroma increases (the production and release of organic volatile compounds). Actually, the level of information of molecular events at the transcriptional, biochemical, hormonal, and metabolite levels underlying ripening in Prunus fruits has increased considerably. However, we still poorly understand the molecular switch that occurs during the transition from unripe to ripe fruits. The objective of this review was to analyze of the molecular bases of fruit quality in Prunus species through an integrated metabolic, genomic, transcriptomic, and epigenetic approach to better understand the molecular switch involved in the ripening process with important consequences from a breeding point of view.

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“…With the exception of fruit skin color, which is controlled by a single gene differentiating red skin and blushed skin cherries 31 , the other studied traits are highly polygenic. Major QTLs were detected for important agronomic traits such as bloom date and its components, chilling and heat requirements 32 – 34 , maturity date 35 , 36 , fruit weight (FW) 36 – 40 , fruit firmness (FF) 36 , 39 – 41 , and fruit sugar content and acidity 36 . Today, several sweet cherry breeding programs are routinely using molecular markers associated to key traits such as self-compatibility, fruit weight, fruit skin color, or maturity date, but no breeder has yet implemented marker-assisted selection for fruit cracking tolerance 30 , 42 – 44 .…”

Section: Introductionmentioning

confidence: 99%

Multi-year analyses on three populations reveal the first stable QTLs for tolerance to rain-induced fruit cracking in sweet cherry (Prunus avium L.)

et al. 2021

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Rain-induced fruit cracking is a major problem in sweet cherry cultivation. Basic research has been conducted to disentangle the physiological and mechanistic bases of this complex phenomenon, whereas genetic studies have lagged behind. The objective of this work was to disentangle the genetic determinism of rain-induced fruit cracking. We hypothesized that a large genetic variation would be revealed, by visual field observations conducted on mapping populations derived from well-contrasted cultivars for cracking tolerance. Three populations were evaluated over 7–8 years by estimating the proportion of cracked fruits for each genotype at maturity, at three different areas of the sweet cherry fruit: pistillar end, stem end, and fruit side. An original approach was adopted to integrate, within simple linear models, covariates potentially related to cracking, such as rainfall accumulation before harvest, fruit weight, and firmness. We found the first stable quantitative trait loci (QTLs) for cherry fruit cracking, explaining percentages of phenotypic variance above 20%, for each of these three types of cracking tolerance, in different linkage groups, confirming the high complexity of this trait. For these and other QTLs, further analyses suggested the existence of at least two-linked QTLs in each linkage group, some of which showed confidence intervals close to 5 cM. These promising results open the possibility of developing marker-assisted selection strategies to select cracking-tolerant sweet cherry cultivars. Further studies are needed to confirm the stability of the reported QTLs over different genetic backgrounds and environments and to narrow down the QTL confidence intervals, allowing the exploration of underlying candidate genes.

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Fruit size and firmness QTL alleles of breeding interest identified in a sweet cherry ‘Ambrunés’ × ‘Sweetheart’ population

Cited by 18 publications

References 67 publications

Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci Linked to Fruit Quality Traits in Apricots Using Specific-Locus Amplified Fragment Sequencing

Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci Linked to Fruit Quality Traits in Apricots Using Specific-Locus Amplified Fragment Sequencing

Molecular Bases of Fruit Quality in Prunus Species: An Integrated Genomic, Transcriptomic, and Metabolic Review with a Breeding Perspective

Multi-year analyses on three populations reveal the first stable QTLs for tolerance to rain-induced fruit cracking in sweet cherry (Prunus avium L.)

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