Sweet characterisation of prostate specific antigen using electrochemical lectin‐based immunosensor assay and MALDI TOF/TOF analysis: Focus on sialic acid

Pihíková, Dominika; Pakanová, Zuzana; Nemčovič, Marek; Baráth, Péter; Belický, Štefan; Bertók, Tomáš; Kasák, Peter; Mucha, Ján; Tkac, Jan

doi:10.1002/pmic.201500463

Cited by 31 publications

(22 citation statements)

References 62 publications

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“…It is a technique used for the level-free impedimetric detection with a lectin and antibody in a sandwich configuration. It is applied for the detection as well as for the glycoprofiling (Zhang et al, 2010b;Pihikova et al, 2016).…”

Section: Plant Lectin-mediated Mitogenic Stimulation Of Lymphocytesmentioning

confidence: 99%

Structure-function and application of plant lectins in disease biology and immunity

Mishra

Behura

Mawatwal

et al. 2019

Food and Chemical Toxicology

140

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A B S T R A C TLectins are proteins with a high degree of stereospecificity to recognize various sugar structures and form reversible linkages upon interaction with glyco-conjugate complexes. These are abundantly found in plants, animals and many other species and are known to agglutinate various blood groups of erythrocytes. Further, due to the unique carbohydrate recognition property, lectins have been extensively used in many biological functions that make use of protein-carbohydrate recognition like detection, isolation and characterization of glycoconjugates, histochemistry of cells and tissues, tumor cell recognition and many more. In this review, we have summarized the immunomodulatory effects of plant lectins and their effects against diseases, including antimicrobial action. We found that many plant lectins mediate its microbicidal activity by triggering host immune responses that result in the release of several cytokines followed by activation of effector mechanism. Moreover, certain lectins also enhance the phagocytic activity of macrophages during microbial infections. Lectins along with heat killed microbes can act as vaccine to provide long term protection from deadly microbes. Hence, lectin based therapy can be used as a better substitute to fight microbial diseases efficiently in future.

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Section: Plant Lectin-mediated Mitogenic Stimulation Of Lymphocytesmentioning

confidence: 99%

Structure-function and application of plant lectins in disease biology and immunity

Mishra

Behura

Mawatwal

et al. 2019

Food and Chemical Toxicology

140

View full text Add to dashboard Cite

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“…EIS was successfully applied as a label‐free and non‐destructive method to detection of the PSA cancer biomarker with low LODs ,. The impedimetric biosensor based on recombinant single‐chain antibody fragments (scAb), fragments covalently immobilized on the gold electrode surface, patterned by a mixed SAM using standard amine‐coupling chemistry was developed .…”

Section: Eis As a Powerful Tool For Sensitive Detection Of Cancer Biomentioning

confidence: 99%

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

et al. 2018

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affinity biosensors are, without any doubt, among the most sensitive analytical devices available, offering low limits of detection and wide linear response ranges. There are, however, only a few papers detailing the application of impedimetric biosensors for the analysis of clinically relevant samples with due clinical performance. The fact that these devices have not found their way to any commercial or clinical use to date might be surprising, since an electrochemical assay platform based on portable potentiostats is a success story for monitoring a range of clinical parameters such as ions, haematological indicators and glucose. This review discusses the reasons behind this discrepancy and addresses the barriers to be overcome in order to achieve the point-of-care diagnostics using such devices for detection of protein oncomarkers approved by FDA. The final part of the review covers the most recent progress in the area.[a] Dr.

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“…These two markers did not have a significant impact on the analysis performance, Table S4: Number of missed cancers by Gleason score for glycan-based biomarkers, PHI and fPSA% (90% sensitivity). References [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ] are cited in the Supplementary File.…”

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confidence: 99%

Validating fPSA Glycoprofile as a Prostate Cancer Biomarker to Avoid Unnecessary Biopsies and Re-Biopsies

Bertók

Jáné

Bertóková

et al. 2020

Cancers

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Background: To compare the clinical performance of a new PCa serum biomarker based on fPSA glycoprofiling to fPSA% and PHI. Methods: Serum samples from men who underwent prostate biopsy due to increased PSA were used. A comparison between two equal groups (with histologically confirmed PCa or benign, non-cancer condition) was used for the clinical validation of a new glycan-based PCa oncomarker. SPSS and R software packages were used for the multiparametric analyses of the receiver operating curve (ROC) and for genetic algorithm metaheuristics. Results: When comparing the non-cancer and PCa cohorts, the combination of four fPSA glycoforms with two clinical parameters (PGI, prostate glycan index (PGI)) showed an area under receiver operating curve (AUC) value of 0.821 (95% CI 0.754–0.890). AUC values were 0.517 for PSA, 0.683 for fPSA%, and 0.737 for PHI. A glycan analysis was also applied to discriminate low-grade tumors (GS = 6) from significant tumors (GS ≥ 7). Conclusions: Compared to PSA on its own, or fPSA% and the PHI, PGI showed improved discrimination between presence and absence of PCa and in predicting clinically significant PCa. In addition, the use of PGI would help practitioners avoid 63.5% of unnecessary biopsies, while the use of fPSA% and PHI would help avoid 17.5% and 33.3% of biopsies, respectively, while missing four significant tumors (9.5%).

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Sweet characterisation of prostate specific antigen using electrochemical lectin‐based immunosensor assay and MALDI TOF/TOF analysis: Focus on sialic acid

Cited by 31 publications

References 62 publications

Structure-function and application of plant lectins in disease biology and immunity

Structure-function and application of plant lectins in disease biology and immunity

Electrochemical Impedance Spectroscopy Based Biosensors: Mechanistic Principles, Analytical Examples and Challenges towards Commercialization for Assays of Protein Cancer Biomarkers

Validating fPSA Glycoprofile as a Prostate Cancer Biomarker to Avoid Unnecessary Biopsies and Re-Biopsies

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