Sweeping under controlled electroosmotic flow and micellar electrokinetic chromatography for on-line concentration and determination of trace phlorizin and quercitrin in urine samples

Zhang, Xiaowei; Zhang, Zhaoxiang

doi:10.1016/j.jpba.2011.05.016

Cited by 19 publications

(4 citation statements)

References 28 publications

(30 reference statements)

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“…Results were calculated from the calibration curve. Determination of Phl in urine sample, based on the previously reported literature (Zhang and Zhang 2011), is important due to the possibility of extraction from the body through the urine. Different concentrations of Phl were found in urine and due to that urine samples were considered as suitable for investigation of matrix effect.…”

Section: Sample Analysismentioning

confidence: 99%

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

et al. 2017

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A boron-doped diamond electrode was used as an electrochemical sensor for the determination of phlorizin (aka phloridzin, phlorrhizin) using square wave voltammetry (SWV). Phlorizin (Phl) exhibited a well-defined oxidation peak at +0.9 V (versus Ag/AgCl electrode 3 M KCl) in solutions with a pH value of 6.0. Parameters such as pH value and scan rate were optimized for cyclic voltammetry as well as amplitude and frequency for SWV. The sensor gave excellent response with a wide linear dynamic range for concentrations of phlorizin from 3 to 100 μM with a detection limit of 0.23 μM and a good repeatability (± 0.9%, n = 7 measurements, c = 10 μM). The effect of interferences by most common compounds was tested, and the method was successfully applied to the determination of the title compound in apple root extracts and urine samples with satisfactory recovery.

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Section: Sample Analysismentioning

confidence: 99%

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

et al. 2017

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“…12,13 To reduce sample dispersion, EOF is typically suppressed or reduced with application of coating to the microchannel 14 or additive to the BGE. 15 In contrast, there are methodologies which exploit EOF to achieve the desired ion pre-concentration and transport effect [16][17][18] in a micro/nanofluidic channel.…”

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confidence: 99%

Electroosmotic flow hysteresis for dissimilar ionic solutions

Lim

Lam

2015

Biomicrofluidics

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Electroosmotic flow (EOF) with two or more fluids is commonly encountered in various microfluidics applications. However, no investigation has hitherto been conducted to investigate the hysteretic or flow direction-dependent behavior during the displacement flow of solutions with dissimilar ionic species. In this investigation, electroosmotic displacement flow involving dissimilar ionic solutions was studied experimentally through a current monitoring method and numerically through finite element simulations. The flow hysteresis can be characterized by the turning and displacement times; turning time refers to the abrupt gradient change of current-time curve while displacement time is the time for one solution to completely displace the other solution. Both experimental and simulation results illustrate that the turning and displacement times for a particular solution pair can be directional-dependent, indicating that the flow conditions in the microchannel are not the same in the two different flow directions. The mechanics of EOF hysteresis was elucidated through the theoretical model which includes the ionic mobility of each species, a major governing parameter. Two distinct mechanics have been identified as the causes for the EOF hysteresis involving dissimilar ionic solutions: the widening/sharpening effect of interfacial region between the two solutions and the difference in ion concentration distributions (and thus average zeta potentials) in different flow directions. The outcome of this investigation contributes to the fundamental understanding of flow behavior in microfluidic systems involving solution pair with dissimilar ionic species. V C 2015 AIP Publishing LLC. [http://dx

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“…However, despite the many types of modes for complicated systems, the largest limitation of CE is that it lacks the ability to discriminate compounds with close charge‐to‐mass ratios. Therefore, to enhance the separation capability of CE, cyclodextrins (CDs) (Yuan et al , ), proteins (Zhang et al , ), surfactants (Zhang and Zhang, ) and organic solvents have been added to the background electrolyte (BGE) as modifiers to separate the complicated samples (Yang et al , ). CDs have hydrophobic inner cavity and hydrophilic outer wall, allowing them to form inclusion complexes with various organic compounds in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier

Gao

Wang

Yang

et al. 2014

Biomedical Chromatography

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The method of capillary zone electrophoresis (CZE) with direct UV detection was developed for the determination of oleanolic acid, ursolic acid, quercetin and apigenin. and then for the first time successfully applied to the analysis of four analytes in Swertia mussotii Franch and its preparations. Various factors affecting the CZE procedure were investigated and optimized, and the optimal conditions were: 50 × 10(-3) mol/L borate-phosphate buffer (pH 9.5) with 5.0 × 10(-3) mol/L β-cyclodextrin, 15 kV separation voltage, 20 °C column temperature, 250 nm detection wavelength and 5 s electrokinetic injection time (voltage 20 psi). Under the conditions, oleanolic acid, ursolic acid, quercetin and apigenin could be determined within the test ranges with a good correlation coefficient (r(2) > 0.9991). The limits of detection for conditions, oleanolic acid, ursolic acid, quercetin and apigenin were 0.3415, 0.2003, 0.0062 and 0.2538 µg/mL, respectively, and the intra- and inter-day relative standard deviations were no more than 4.72%. This procedure provided a convenient, sensitive and accurate method for simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in S. mussotii Franch.

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Sweeping under controlled electroosmotic flow and micellar electrokinetic chromatography for on-line concentration and determination of trace phlorizin and quercitrin in urine samples

Cited by 19 publications

References 28 publications

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

Electroosmotic flow hysteresis for dissimilar ionic solutions

Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier

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