Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier

Abstract

The method of capillary zone electrophoresis (CZE) with direct UV detection was developed for the determination of oleanolic acid, ursolic acid, quercetin and apigenin. and then for the first time successfully applied to the analysis of four analytes in Swertia mussotii Franch and its preparations. Various factors affecting the CZE procedure were investigated and optimized, and the optimal conditions were: 50 × 10(-3) mol/L borate-phosphate buffer (pH 9.5) with 5.0 × 10(-3) mol/L β-cyclodextrin, 15 kV separation voltage, 20 °C column temperature, 250 nm detection wavelength and 5 s electrokinetic injection time (voltage 20 psi). Under the conditions, oleanolic acid, ursolic acid, quercetin and apigenin could be determined within the test ranges with a good correlation coefficient (r(2) > 0.9991). The limits of detection for conditions, oleanolic acid, ursolic acid, quercetin and apigenin were 0.3415, 0.2003, 0.0062 and 0.2538 µg/mL, respectively, and the intra- and inter-day relative standard deviations were no more than 4.72%. This procedure provided a convenient, sensitive and accurate method for simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in S. mussotii Franch.

Flavonoids biosynthesis in plants and its further analysis by capillary electrophoresis

“…Gao et al. developed capillary zone electrophoresis (CZE) with direct UV detector for the determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch . This method is considered to be accurate method for simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in this plant.…”

Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β‐cyclodextrin by capillary electrophoresis

“…The PTAs in FF was extracted by the organic solvent and finally transferred into the BGE for CE analysis. The extraction procedure was referred to the previous studies . Briefly, the fruits were powdered, homogenized, and dried 6 hours in the oven at 50°C.…”

“…Certain types of PTAs in some Chinese herbs, such as Prunella vulgaris L . , Swerita mussotii and Pterocephalus hookeri , could be detected by capillary zone electrophoresis (CZE) or micellar electrokinetic chromatography (MEKC) . Otherwise, Chen's group also reported that three bioactive triterpenes were simultaneously separated by nonaqueous capillary electrophoresis (NACE) .…”

Determination of Ursolic Acid in Extracts From Ligustri lucidum Fruit Using an Electrochemical Method

“…Thus far, several methods have been developed for the determination of UA, including micellar electrokinetic capillary chromatography (Zhang et al, 2005), HPLC (Li et al, 2019), LC-MS (Novotny et al, 2003), capillary zone electrophoresis (Gao et al, 2015b) and UV spectroscopy (Pironi et al, 2018). These methods all require complicated sample preparation procedures and a sophisticated operation.…”