Flavonoids biosynthesis in plants and its further analysis by capillary electrophoresis

Singh, Baljinder; Kumar, Ashwini; Malik, Ashok Kumar

doi:10.1002/elps.201600334

Cited by 49 publications

(24 citation statements)

References 89 publications

(58 reference statements)

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“…MAE+UAE was a good alternative for the higher extraction yield and extractable bioactive compounds of TFPF that had potential application prospect in pharmaceutical production of treating inflammation and other related fields. For the further study in analysis of flavonoids and bioactive compounds of TFPF, capillary electrophoresis (CE) and capillary electrochromatography (CEC) will be alternative because of their efficient analytical techniques . According to the reviews by Chen, X. J. et al and Zhao, J. et al that molecules and particles, such as organic/inorganic, charged/uncharged, micro/macromolecules could be successfully analyzed by CE and CEC, due to their versatility and high efficiency, which have attracted great interest of researchers in phytochemical analysis.…”

Section: Discussionmentioning

confidence: 99%

Optimized microwave‐assistant extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. and evaluation of its anti‐allergic activity

et al. 2017

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Microwave extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. was investigated in this study, extraction process was first performed in an ultrasonic cleaner, then treated by microwave irradiation. The optimum ultrasonic time of 25 min was selected by single-factor experiments. A response surface methodology has been used to obtain a mathematical model that describes the process and analyzes the significant parameters ethanol concentration 59.92%, liquid to raw materials ratio 21.24 mL/g, microwave radiation time 209.53 s, and microwave power 274.14 w. In these optimum conditions, the yield of flavonoids from P. forrestii (TFPF) could be up to 9.11 ± 0.08%, which was increased by 14.30 and 19.86% compared microwave extraction and ultrasonic extraction, respectively. In vitro suppress hyaluronidase experimentation showed that TFPF purified using polyamide exhibited good anti-hyaluronidase ability with IC value of 1.033 mg/mL, possessing certain anti-antiallergic and potential application prospect in pharmaceutical production of treating inflammation and other related fields.

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Section: Discussionmentioning

confidence: 99%

Optimized microwave‐assistant extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. and evaluation of its anti‐allergic activity

et al. 2017

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“…We assume that over expression of CYP75B1 and CYP73A might contribute to the cold resistance of C. oleifera 'Huaxin' by mediating the synthesis of phenylpropanoid. Flavonoids are other plant products that have multiple biological functions, and their accumulation usually occurs in plants when they are subjected to abiotic stresses [61]. Anthocyanins and flavonols are two of the most important flavonoids that accumulate in response to low temperatures [62,63].…”

Section: Other Important Degs Responding To Low Temperaturementioning

confidence: 99%

Transcriptomic Analyses of Camellia oleifera ‘Huaxin’ Leaf Reveal Candidate Genes Related to Long-Term Cold Stress

et al. 2020

IJMS

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‘Huaxin’ is a new high-yielding timber cultivar of Camellia oleifera of high economic value, and has been widely cultivated in the red soil hilly region of Hunan Province of the People´s Republic of China in recent years. However, its quality and production are severely affected by low temperatures during flowering. To find genes related to cold tolerance and further explore new candidategenes for chilling-tolerance, Illumina NGS (Next Generation Sequencing) technology was used to perform transcriptomic analyses of C. oleifera ‘Huaxin’ leaves under long-term cold stress. Nine cDNA libraries were sequenced, and 58.31 Gb high-quality clean reads were obtained with an average of 5.92 Gb reads for each sample. A total of 191,150 transcripts were obtained after assembly. Among them, 100,703 unigenes were generated, and 44,610 unigenes were annotated. In total, 1564 differentially expressed genes (DEGs) were identified both in the A_B and A_C gene sets. In the current study, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, andrevealed a group of cold-responsive genes related to hormone regulation, photosynthesis, membrane systems, and osmoregulation; these genes encoded many key proteins in plant biological processes, such as serine/threonine-protein kinase (STPK), transcription factors (TFs), fatty acid desaturase (FAD), lipid-transfer proteins (LTPs), soluble sugars synthetases, and flavonoid biosynthetic enzymes. Some physiological indicators of C. oleifera ‘Huaxin’ were determined under three temperature conditions, and the results were consistent with the molecular sequencing. In addition, the expression levels of 12 DEGs were verified using quantitative real-time polymerase chain reaction (qRT-PCR). In summary, the results of DEGs analysis together with qRT-PCR tests contribute to the understanding of cold tolerance and further exploring new candidate genes for chilling-tolerance in molecular breeding programs of C. oleifera ‘Huaxin’.

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“…Separation and analysis of polyphenols in herbs and spices by CE involves separation based on the electrophoretic mobilities of a solution that consists of electrically charged species, in small-diameter capillaries [ 92 ] it is recognized as being effective in phenolic characterization, offering practical operation, rapid analysis, low consumption of solvent, and low cost. This method represents a valuable alternative to HPLC in the separation of closely associated phenolics, but its major drawbacks are its lower reproducibility and sensitivity as compared to HPLC [ 93 ].…”

Section: Chromatographic Techniques With Ultraviolet/visible (Uv/vmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

Tzima

Brunton

Dilip

2018

Plants

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Lamiaceae species are promising potential sources of natural antioxidants, owing to their high polyphenol content. In addition, increasing scientific and epidemiological evidence have associated consumption of foods rich in polyphenols with health benefits such as decreased risk of cardiovascular diseases mediated through anti-inflammatory effects. The complex and diverse nature of polyphenols and the huge variation in their levels in commonly consumed herbs make their analysis challenging. Innovative robust analytical tools are constantly developing to meet these challenges. In this review, we present advances in the state of the art for the identification and quantification of polyphenols in Lamiaceae species. Novel chromatographic techniques that have been employed in the past decades are discussed, ranging from ultra-high-pressure liquid chromatography to hyphenated spectroscopic methods, whereas performance characteristics such as selectivity and specificity are also summarized.

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Flavonoids biosynthesis in plants and its further analysis by capillary electrophoresis

Cited by 49 publications

References 89 publications

Optimized microwave‐assistant extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. and evaluation of its anti‐allergic activity

Optimized microwave‐assistant extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. and evaluation of its anti‐allergic activity

Transcriptomic Analyses of Camellia oleifera ‘Huaxin’ Leaf Reveal Candidate Genes Related to Long-Term Cold Stress

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

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