Sweat Based‐multiplexed Detection of NPY‐Cortisol for Disease Diagnostics and Stress Management

Churcher, Nathan Kodjo Mintah; Upasham, Sayali; Rice, Paul; Greyling, Cornelia Felicia; Prasad, Shalini

doi:10.1002/elan.202100083

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…While the etiology and pathways of stress modulation are still under active research being able to monitor simultaneously, the modulation of these stress biomarkers concerning different stress conditions can prove helpful, especially for chronic stress, which requires monitoring. While various research groups have looked into the combinatorial detection of cortisol and NPY toward stress (Antonijevic et al, 2000; Held et al, 2006; Kapritsou et al, 2017; Morgan et al, 2001, 2002; Seedat et al, 2003; Westrin et al, 1999), to the best of our knowledge, there has not been any reported research to the best of our knowledge that has carried out these experiments in sweat apart from Churcher, Greyling, et al (2022); Churcher, Upasham, et al (2022).…”

Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 97%

“…In Churcher, Upasham, et al (2022), the authors have demonstrated a platform capable of simultaneously detecting cortisol and NPY using an antibody affinity‐based immunoassay platform in synthetic sweat (with different pH.) and in pooled human sweat.…”

Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 99%

“…The group demonstrated a robust calibrated response curve within the ranges reported for cortisol and NPY, respectively. In Churcher, Upasham, et al (2022), the group demonstrated a portable device that can quantify cortisol and NPY using an aptamer‐based platform this time for increased specificity and sensitivity. Herein, the authors also demonstrated the comparison of the platform to detect a change in cortisol and NPY in real‐life samples with ELISA validation of the results obtained.…”

Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 99%

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Passive sweat wearable: A new paradigm in the wearable landscape toward enabling “detect to treat” opportunities

Greyling

Ganguly

Sardesai

et al. 2023

WIREs Nanomed Nanobiotechnol

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Growing interest over recent years in personalized health monitoring coupled with the skyrocketing popularity of wearable smart devices has led to the increased relevance of wearable sweat‐based sensors for biomarker detection. From optimizing workouts to risk management of cardiovascular diseases and monitoring prediabetes, the ability of sweat sensors to continuously and noninvasively measure biomarkers in real‐time has a wide range of applications. Conventional sweat sensors utilize external stimulation of sweat glands to obtain samples, however; this stimulation influences the expression profile of the biomarkers and reduces the accuracy of the detection method. To address this limitation, our laboratory pioneered the development of the passive sweat sensor subfield, which allowed for our progress in developing a sweat chemistry panel. Passive sweat sensors utilize nanoporous structures to confine and detect biomarkers in ultra‐low sweat volumes. The ability of passive sweat sensors to use smaller samples than conventional sensors enable users with sedentary lifestyles who perspire less to benefit from sweat sensor technology not previously afforded to them. Herein, the mechanisms and strategies of current sweat sensors are summarized with an emphasis on the emerging subfield of passive sweat‐based diagnostics. Prospects for this technology include discovering new biomarkers expressed in sweat and expanding the list of relevant detectable biomarkers. Moreover, the accuracy of biomarker detection can be enhanced with machine learning using prediction algorithms trained on clinical data. Applying this machine learning in conjunction with multiplex biomarker detection will allow for a more holistic approach to trend predictions.This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Diagnostic Tools > Biosensing

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Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 97%

Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 99%

Section: Emerging Perspective Of Application Of Nanotechnology Based ...mentioning

confidence: 99%

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Passive sweat wearable: A new paradigm in the wearable landscape toward enabling “detect to treat” opportunities

Greyling

Ganguly

Sardesai

et al. 2023

WIREs Nanomed Nanobiotechnol

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“…SPR has also been used to characterize the immobilization of an affinity reagent, rather than testing binding to the target itself. Churcher, Upasham, and coworkers utilized SPR for the real-time monitoring of antibodies onto a Protein A functionalized surface, to verify they could attach the molecule of interest to a biosensor that relied on a different method of detection [ 220 , 221 , 222 ]. While many papers use SPR to supplement other investigations, some studies focused on developing biosensors based entirely on the technique of LSPR itself [ 34 , 45 ].…”

Section: Beyond Simple Kinetics: the Versatility Of Opensprmentioning

confidence: 99%

Application of the Nicoya OpenSPR to Studies of Biomolecular Binding: A Review of the Literature from 2016 to 2022

Hanson

Whelan

2023

Sensors

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The Nicoya OpenSPR is a benchtop surface plasmon resonance (SPR) instrument. As with other optical biosensor instruments, it is suitable for the label-free interaction analysis of a diverse set of biomolecules, including proteins, peptides, antibodies, nucleic acids, lipids, viruses, and hormones/cytokines. Supported assays include affinity/kinetics characterization, concentration analysis, yes/no assessment of binding, competition studies, and epitope mapping. OpenSPR exploits localized SPR detection in a benchtop platform and can be connected with an autosampler (XT) to perform automated analysis over an extended time period. In this review article, we provide a comprehensive survey of the 200 peer-reviewed papers published between 2016 and 2022 that use the OpenSPR platform. We highlight the range of biomolecular analytes and interactions that have been investigated using the platform, provide an overview on the most common applications for the instrument, and point out some representative research that highlights the flexibility and utility of the instrument.

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“…However, measurements were only possible as low as 20 nM (4704 pg/mL), which is much higher than needed for brain measurements. Churcher et al reported a detection limit of NPY of 10 and 50 pg/mL in sweat using antibodies and larger electrodes. This is a significant advance in the field, with the main issue being the intrinsic disadvantages of antibodies that are not stable for long-term use and the fact that these electrodes cannot be used in the brain due to their size.…”

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confidence: 99%

Development of a Neuropeptide Y-Sensitive Implantable Microelectrode for Continuous Measurements

Fernández-Vega,

Meléndez-Rodríguez,

Ospina-Alejandro

et al. 2024

ACS Sens.

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In this work, we present the development of the first implantable aptamer-based platinum microelectrode for continuous measurement of a nonelectroactive molecule, neuropeptide Y (NPY). The aptamer immobilization was performed via conjugation chemistry and characterized using cyclic voltammetry before and after the surface modification. The redox label, methylene blue (MB), was attached at the end of the aptamer sequence and characterized using square wave voltammetry (SWV). NPY standard solutions in a three-electrode cell were used to test three aptamers in steady-state measurement using SWV for optimization. The aptamer with the best performance in the steady-state measurements was chosen, and continuous measurements were performed in a flow cell system using intermittent pulse amperometry. Dynamic measurements were compared against confounding and similar peptides such as pancreatic polypeptide and peptide YY, as well as somatostatin to determine the selectivity in the same modified microelectrode. Our Pt-microelectrode aptamer-based NPY biosensor provides signals 10 times higher for NPY compared to the confounding molecules. This proof-of-concept shows the first potential implantable microelectrode that is selectively sensitive to NPY concentration changes.

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Sweat Based‐multiplexed Detection of NPY‐Cortisol for Disease Diagnostics and Stress Management

Cited by 10 publications

References 25 publications

Passive sweat wearable: A new paradigm in the wearable landscape toward enabling “detect to treat” opportunities

Passive sweat wearable: A new paradigm in the wearable landscape toward enabling “detect to treat” opportunities

Application of the Nicoya OpenSPR to Studies of Biomolecular Binding: A Review of the Literature from 2016 to 2022

Development of a Neuropeptide Y-Sensitive Implantable Microelectrode for Continuous Measurements

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