Application of the Nicoya OpenSPR to Studies of Biomolecular Binding: A Review of the Literature from 2016 to 2022

Abstract

The Nicoya OpenSPR is a benchtop surface plasmon resonance (SPR) instrument. As with other optical biosensor instruments, it is suitable for the label-free interaction analysis of a diverse set of biomolecules, including proteins, peptides, antibodies, nucleic acids, lipids, viruses, and hormones/cytokines. Supported assays include affinity/kinetics characterization, concentration analysis, yes/no assessment of binding, competition studies, and epitope mapping. OpenSPR exploits localized SPR detection in a benchtop platform and can be connected with an autosampler (XT) to perform automated analysis over an extended time period. In this review article, we provide a comprehensive survey of the 200 peer-reviewed papers published between 2016 and 2022 that use the OpenSPR platform. We highlight the range of biomolecular analytes and interactions that have been investigated using the platform, provide an overview on the most common applications for the instrument, and point out some representative research that highlights the flexibility and utility of the instrument.

Peptide-based self-assembled monolayers (SAMs): what peptides can do for SAMs and vice versa

“…SPR is an optical technique used to quantify molecular interactions in real time on a metal thin film due to alterations on its refractive index. [195][196][197] SPR technique can quantify the binding of a soluble molecule (analyte) to a ligand bound on the metal thin film with a detection limit of around 0.5 ng cm À2 . 198 The metal thin film, usually Au, must be deposited on a glass prism with a thickness of ca.…”

Pathologic α-Synuclein-NOD2 Interaction and RIPK2 Activation Drives Microglia-Induced Neuroinflammation in Parkinson’s Disease

“…Surface plasmon resonance (SPR), a technology that enables comprehensive and quantitative studies of protein-protein interactions (Hanson and Whelan, 2023), was used to determine the equilibrium binding kinetics between NOD2 and pathologic α-syn (Figure 1H). To ascertain the binding affinity, the Fc-tag from the tagged hNOD2 was removed using HRV3C protease (Figure S3A and S3B).…”

Combining the Benefits of Biotin–Streptavidin Aptamer Immobilization with the Versatility of Ni-NTA Regeneration Strategies for SPR

“…The position of the tag on the ligand offers the ability to control the orientation of the ligand relative to the sensor surface [4]. A review of 200 studies utilizing the Nicoya OpenSPR between 2016 and 2022 included 22 that opted for streptavidin-biotin immobilization [13].…”