Sustained release of vascular endothelial growth factor from calcium-induced alginate hydrogels reinforced by heparin and chitosan

Lee, K.W.; Yoon, Jun Jin; Lee, J.H.; Kim, S.Y.; Jung, Hoeryong; Kim, Seung Ju; Joh, Jae‐Won; Lee, H.H.; Lee, D.S.; Lee, S.K.

doi:10.1016/j.transproceed.2004.08.078

Cited by 80 publications

(46 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…14 In particular, the calciumalginate microcapsule coated by chitosan via electrostatic interactions between carboxyl groups in alginate and amine groups in chitosan has been widely studied. Many reports [15][16][17][18][19] demonstrate the effectiveness of chitosan-coated alginate microcapsules for sustained drug release and for live cell therapy. Several research groups have investigated the alginate-chitosan complexation 17,20 -24 including the kinetic binding of chitosan to the alginate beads, the effects of chitosan characteristics and preparation parameters on microcapsule properties, and the diffusion mechanism of the enclosed drugs.…”

Section: Microencapsulationmentioning

confidence: 99%

Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization

Chen

Ouyang

Lawuyi

et al. 2005

J Biomedical Materials Res

View full text Add to dashboard Cite

This study investigates the fluorogenic characteristics of the chitosan-genipin reaction for applications in microencapsulation research. Results showed that the chitosan-genipin reaction generated a colored and fluorescent product, with optimal excitation and emission wavelengths at 369 and 470 nm, respectively. Furthermore, it was found that reaction conditions affected the fluorescence intensity of the product. Mixture at the ratio of 4:1 (chitosan: genipin by weight) fluoresced the most. It also fluoresced stronger if the reaction occurred at higher temperature, with the intensity of 10.4 x 10(5) CPS at 37 degrees C, 5.9 x 10(5) CPS at 20 degrees C, and 2.5 x 10(5) CPS at 4 degrees C. As well, the fluorescence of the mixture developed gradually over time, attaining the emission maxima of 2.9 x 10(5), 7.6 x 10(5), and 10.0 x 10(5) CPS in 1, 6, and 18 h, respectively. Chitosan-coated alginate microcapsules were prepared without prior labeling, to which subsequent genipin treatment was applied in order to examine the potential of using genipin in microcapsule characterization. Chitosan bound to the alginate beads interacted with genipin, from which the resultant fluorescent signals allowed for clear visualization of the chitosan coating under confocal laser scanning microscopy. The relative fluorescence intensity across the chitosan membrane was found to be considerably higher than the controls (175 vs. 50). The membrane thickness measured was 29.2 +/- 7.3 microm. These findings demonstrate a convenient and effective way of characterizing chitosan-based microcapsules using genipin as a fluorogenic marker, a technique that will be useful in microcapsule research and other biomedical applications.

show abstract

Section: Microencapsulationmentioning

confidence: 99%

Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization

Chen

Ouyang

Lawuyi

et al. 2005

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Bioactive or biomimetic hydrogels suitable for drug delivery and tissue engineering applications can be fabricated from naturally derived materials such as alginate [1], glycosaminoglycans [2][3][4][5], fibrin [6,7], chitosan [8][9][10], dextran [11], collagen [12], and gelatin [13,14]. Although hydrogels derived from natural matrices are inherently bioactive, it remains challenging in tuning their bioactivity, mechanics, and degradation features [15].…”

Section: Introductionmentioning

confidence: 99%

Improving gelation efficiency and cytocompatibility of visible light polymerized thiol-norbornene hydrogels via addition of soluble tyrosine

2017

View full text Add to dashboard Cite

Hydrogels immobilized with biomimetic peptides have been used widely for tissue engineering and drug delivery applications. Photopolymerization has been among the most commonly used techniques to fabricate peptide-immobilized hydrogels as it offers rapid and robust peptide immobilization within a crosslinked hydrogel network. Both chain-growth and stepgrowth photopolymerizations can be used to immobilized peptides within covalently crosslinked 3

show abstract

“…There are several medical applications for alginate gels. For example, pure or chitosan/heparin-coated alginate microbeads are used for drug delivery [18]. Gel-cell composites were also supposed to be directly injected into the body, e.g.…”

Section: Resultsmentioning

confidence: 99%

Alginate/calcium phosphate scaffolds with oriented, tube-like pores

Despang¹,

Borner²,

Dittrich³

et al. 2005

Mat.-wiss. u. Werkstofftech.

View full text Add to dashboard Cite

Porous and mineralised scaffolds are required for various applications in hard tissue engineering. Scaffolds with oriented tube-like pores facilitate homogenous cell seeding, a sufficient nutrient supply during cell culture (even in big constructs) and a fast vascularisation after implantation. The phenomenon of ionotropic gelation has been known since more than 30 years which describes that alginate forms gels with capillary-like pores when covered with solutions of di-or trivalent cations [1]. This technique has been used here to develop scaffolds with tube-like and regular pores from alginate/calcium phosphate composites and to stabilise them by mineralisation with hydroxyapatite from solution.

show abstract

Sustained release of vascular endothelial growth factor from calcium-induced alginate hydrogels reinforced by heparin and chitosan

Cited by 80 publications

References 3 publications

Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization

Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization

Improving gelation efficiency and cytocompatibility of visible light polymerized thiol-norbornene hydrogels via addition of soluble tyrosine

Alginate/calcium phosphate scaffolds with oriented, tube-like pores

Contact Info

Product

Resources

About