Sustained delivery of chondroitinase ABC by poly(propylene carbonate)-chitosan micron fibers promotes axon regeneration and functional recovery after spinal cord hemisection

Ni, Shilei; Xia, Tongliang; Li, Xingang; Zhu, Xiaodong; Qi, Hongxu; Huang, Shanying; Wang, Jiangang

doi:10.1016/j.brainres.2015.08.018

Cited by 22 publications

(14 citation statements)

References 28 publications

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“…All these mechanisms may be involved in the BBB decrease observed in SCI rats and the recovery of neuromotor function following HBO + enzyme treatment in the present study. ChABC enzyme administration may directly overcome the endothelial barrier through the degradation of CSPG ( 33 ), thus creating a permeable environment that allows axonal regeneration ( 34 , 35 ). A previous study indicated that enzyme secretion begins within a day of injury and reaches its maximum expression following 1 week ( 11 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury

Liu

Wang

et al. 2018

Mol Med Report

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The present study aimed to investigate the effect of combined hyperbaric oxygen (HBO) and chondroitinase ABC (ChABC) enzyme therapy in a rat model of spinal cord injury (SCI) and to explore the underlying mechanisms. A total of 48 healthy male Wistar rats were randomly divided into six groups: Sham, SCI, vehicle, HBO, ChABC enzyme and HBO + ChABC. Excluding the sham group, SCI was established in rats by a clip compression injury and rats subsequently received HBO treatment for 2 weeks with or without an intraspinal injection of 0.1 U/µl ChABC. Neuromotor functions were examined using the Basso-Beattie-Bresnahan locomotor rating scale and the inclined plane assessment at baseline and for 4 weeks following SCI establishment. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were also measured, in addition to the expression of glycogen synthase kinase-3β (GSK3β) and aquaporin 4 (AQP4). Results revealed that combined HBO and ChABC treatment significantly improved neuromotor function compared with the HBO or ChABC treatments alone. HBO and/or ChABC treatment significantly increased SOD and decreased MDA levels, as well as GSK3β expression, compared with the sham and SCI rats. The combined HBO and ChABC treatment significantly inhibited SCI-induced AQP4 expression, but ChABC alone did not. Functional recovery in the HBO + ChABC group was significantly increased compared with the HBO or ChABC groups. These results indicate that combined HBO and ChABC treatment is more effective in treating SCI than either therapy alone.

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Section: Discussionmentioning

confidence: 99%

Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury

Liu

Wang

et al. 2018

Mol Med Report

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“…Neuroprotective treatments aim to avoid or prevent further advancement of subsidiary damage, while neuroregenerative therapies focus on restoring disabled function by repairing the disrupted neural circuit of the spinal cord (Ni et al, 2015). Nonetheless, once the period for treatment intervention to intercept subsequent damage events has elapsed, the focus turns toward the rejuvenation of the damaged spinal cord.…”

Section: Strategies and Challenges For The Treatment Of Scimentioning

confidence: 99%

Polymer-Based Scaffold Strategies for Spinal Cord Repair and Regeneration

Chen

Shu

et al. 2020

Front. Bioeng. Biotechnol.

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The injury to the spinal cord is among the most complex fields of medical development. Spinal cord injury (SCI) leads to acute loss of motor and sensory function beneath the injury level and is linked to a dismal prognosis. Currently, while a strategy that could heal the injured spinal cord remains unforeseen, the latest advancements in polymer-mediated approaches demonstrate promising treatment forms to remyelinate or regenerate the axons and to integrate new neural cells in the SCI. Moreover, they possess the capacity to locally deliver synergistic cells, growth factors (GFs) therapies and bioactive substances, which play a critical role in neuroprotection and neuroregeneration. Here, we provide an extensive overview of the SCI characteristics, the pathophysiology of SCI, and strategies and challenges for the treatment of SCI in a review. This review highlights the recent encouraging applications of polymer-based scaffolds in developing the novel SCI therapy.

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“…Several studies using the enzyme chondroitinase ABC [36][37][38][39] in ratis demonstrated their potential in digesting CSPGs -inhibitory molecules predominant in glial scar -modifying the intra and extracellular architecture, reducing the formation of gliosis, regenerating axons injured by improving neural connections and promoting neuroprotection.…”

Section: Glial Scarmentioning

confidence: 99%

“…In the following Table 1, a summary of the therapies addressed in this review can be observed: 35 Schwann cells Rats Yick 36 , Xia et al 37 , Huang et al 38 , Ni et al 39 Chondroitinase ABC Rats…”

Section: Glial Scarmentioning

confidence: 99%

Glial scar-modulation as therapeutic tool in spinal cord injury in animal models

Orlandin

Ambrósio²,

Lara³

2017

Acta Cir. Bras.

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Purpose: Spinal Cord injury represents, in veterinary medicine, most of the neurological attendances and may result in permanent disability, death or euthanasia. Due to inflammation resulting from trauma, it originates the glial scar, which is a cell interaction complex system. Its function is to preserve the healthy circuits, however, it creates a physical and molecular barrier that prevents cell migration and restricts the neuroregeneration ability. Methods: This review aims to present innovations in the scene of treatment of spinal cord injury, approaching cell therapy, administration of enzyme, anti-inflammatory, and other active principles capable of modulating the inflammatory response, resulting in glial scar reduction and subsequent functional improvement of animals. Results: Some innovative therapies as cell therapy, administration of enzymes, immunosuppressant or other drugs cause the modulation of inflammatory response proved to be a promising tool for the reduction of gliosis Conclusion: Those tools promise to reduce gliosis and promote locomotor recovery in animals with spinal cord injury.

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Sustained delivery of chondroitinase ABC by poly(propylene carbonate)-chitosan micron fibers promotes axon regeneration and functional recovery after spinal cord hemisection

Cited by 22 publications

References 28 publications

Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury

Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury

Polymer-Based Scaffold Strategies for Spinal Cord Repair and Regeneration

Glial scar-modulation as therapeutic tool in spinal cord injury in animal models

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