Polymer-Based Scaffold Strategies for Spinal Cord Repair and Regeneration

Qu, Wenrui; Chen, Bingpeng; Shu, Wentao; Tian, Heng; Ou, Xiaolan; Zhang, Xi; Wang, Yinan; Wu, Minfei

doi:10.3389/fbioe.2020.590549

Cited by 18 publications

(10 citation statements)

References 94 publications

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“…3D nanoscaffolds are usually composites composed of some natural polymers (eg, chitosan, collagen, hyaluronic acid (HA), or fibrin) and synthetic nanomaterials (eg, PLGA, PCL, CNTs, or GO). 103 Natural source nanomaterials can guarantee biocompatibility for stem cell differentiation due to their good biodegradability and low antigenicity, 103 , 104 while synthetic nanomaterials can be modified in terms of surface topography, mechanical properties, and electrical properties, which make them favorable for the differentiation of stem cells into neural cells. 105 , 106 Although 3D nanoscaffolds have shown great application prospects in nerve regeneration for degenerative diseases, nanocomposites are often restricted due to their nonbiodegradability, poorly controlled release of drugs, and limited biocompatibility, thus delaying their clinical application.…”

Section: Combined Nanotherapeutic and Stem Cell Therapeutic Strategie...mentioning

confidence: 99%

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Wei¹,

Yang²,

Li³

2023

IJN

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Neurodegeneration is characterized by progressive, disabling, and incurable neurological disorders with the massive loss of specific neurons. As one of the most promising potential therapeutic strategies for neurodegenerative diseases, stem cell therapy exerts beneficial effects through different mechanisms, such as direct replacement of damaged or lost cells, secretion of neurotrophic and growth factors, decreased neuroinflammation, and activation of endogenous stem cells. However, poor survival and differentiation rates of transplanted stem cells, insufficient homing ability, and difficulty tracking after transplantation limit their further clinical use. The rapid development of nanotechnology provides many promising nanomaterials for biomedical applications, which already have many applications in neurodegenerative disease treatment and seem to be able to compensate for some of the deficiencies in stem cell therapy, such as transport of stem cells/genes/drugs, regulating stem cell differentiation, and real-time tracking in stem cell therapy. Therefore, nanotherapeutic strategies combined with stem cell therapy is a promising therapeutic approach to treating neurodegenerative diseases. The present review systematically summarizes recent advances in stem cell therapeutics and nanotherapeutic strategies and highlights how they can be combined to improve therapeutic efficacy for the treatment of neurodegenerative diseases.

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Section: Combined Nanotherapeutic and Stem Cell Therapeutic Strategie...mentioning

confidence: 99%

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Wei¹,

Yang²,

Li³

2023

IJN

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“…The limited survival and neuronal differentiation rates of transplanted NSCs are the major hurdles for neural regeneration and functional recovery after SCI 23 . In this study, we first demonstrated that the knockout of NF-1 improved the antiapoptotic and neuronal differentiation abilities of NSCs via the enhancement of the mTORC2 signaling pathway in vitro and in vivo, and the transplantation of NSCs with NF-1 knockout increased tissue repair and neurological recovery in rats subjected to SCI.…”

Section: Discussionmentioning

confidence: 99%

Transplanting neurofibromatosis-1 gene knockout neural stem cells improve functional recovery in rats with spinal cord injury by enhancing the mTORC2 pathway

Chen

Zhu

et al. 2022

Exp Mol Med

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The poor survival and low efficiency of neuronal differentiation limits the therapeutic effects of transplanted neural stem cells in the treatment of spinal cord injury. Neurofibromatosis-1 (NF-1) is a tumor suppressor gene that restricts the rapid and abnormal growth and differentiation of neural cells. In the present study, lentiviral vectors were used to knock out NF-1, Ricotr (the core member of mTORC2) or NF-1+Ricotr in neural stem cells in vitro, and the NF-1, Ricotr or NF-1+Ricotr knockout neural stem cells were transplanted at the lesion site in a rat model of spinal cord injury (SCI). We first demonstrated that targeted knockout of NF-1 had an antiapoptotic effect and improved neuronal differentiation by enhancing the mTORC2/Rictor pathway of neural stem cells in vitro. Subsequently, transplanting NF-1 knockout neural stem cells into the injured site sufficiently promoted the tissue repair and functional recovery of rats with spinal cord injury by enhancing the survival and neuronal differentiation of grafted neural stem cells. Collectively, these findings reveal a prominent role of NF-1 in neural stem cell biology, which is an invaluable step forward in enhancing the benefit of neural stem cell-mediated regenerative cell therapy for spinal cord injury and identifies the transplantation of NF-1 knockout neural stem cells as a promising strategy for spinal cord injury.

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“…100 Various other polymer based approaches for spinal cord regeneration have also been recently reviewed. [101][102][103][104] Biomaterials have also emerged as promising therapeutic strategies for cell delivery to the brain. A recent study showed improved activity of MSCs and vascular endothelial cells (ECs) on 3D spheroids as compared to conventional mixed cell suspensions.…”

Section: Central Nervous Systemmentioning

confidence: 99%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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Polymer-Based Scaffold Strategies for Spinal Cord Repair and Regeneration

Cited by 18 publications

References 94 publications

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Transplanting neurofibromatosis-1 gene knockout neural stem cells improve functional recovery in rats with spinal cord injury by enhancing the mTORC2 pathway

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

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