Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts

Abstract

Dedifferentiation and proliferation of endogenous cardiomyocytes in situ can effectively improve cardiac repair following myocardial infarction (MI). 6-Bromoindirubin-3-oxime (BIO) and insulin-like growth factor 1 (IGF-1) are two potent factors that promote cardiomyocyte survival and proliferation. However, their delivery for sustained release in MI-affected areas has proved to be challenging. In the current research, we present a study on the sustained co-delivery of BIO and IGF-1 in a hybrid hydrogel system to simulate endogenous cardiac repair in an MI rat model. Both BIO and IGF-1 were efficiently encapsulated in gelatin nanoparticles, which were later cross-linked with the oxidized alginate to form a novel hybrid hydrogel system. The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function. Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

“…It is worth noting that a functional polyion complex added by static cross-linking create a controlled release system of NO to inflammatory tissues to remove the ROS by redox reaction to promote angiogenesis and prolong the retention period for more than 10 days, which solved the problem of short retention time of natural hydrogel (Vong et al, 2018). Equally, the cross-linking with oxygen-suppressing microspheres to release oxygen to infarcted tissue increase myocardial cell survival rate (Fan et al, 2018). Clearly, the plasticity of synthetic hydrogels provides an effective way to treatment based on cardiovascular disease pathways.…”

Synthesis of Injectable Alginate Hydrogels with Muscle-Derived Stem Cells for Potential Myocardial Infarction Repair

“…SCL hydrogels were synthesized with partially oxidized sodium alginate cross-linking with gelatin, following our previously published procedures [35]. Four hydrogel formulations, namely SCL 0.8%, SCL 1.1%, SCL 1.5%, and SCL 2%, based on their respective sodium alginate concentrations, comprised four experimental groups.…”

A novel double-targeted nondrug delivery system for targeting cancer stem cells

“…19,34 Briefly, the oxidized alginate was obtained by mixing sodium peroxide and sodium alginate (in distilled water) with a mass ratio of 1:2. The reaction was conducted at 4°C for 2 hours and was terminated by the addition of ethylene glycol; then, the oxidized alginate was lyophilized at -20°C and dissolved in distilled water to achieve a 1.4% concentration.…”