Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

Abstract

Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of their production processes can be achieved, in part, by using cheap substrates found among agricultural and food wastes or byproducts. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of surfactin production by Bacillus subtilis #309. The culture medium containing soap-derived waste glycerol led to the best surfactin production, reaching about 2.8 g/L. To the best of our knowledge, this is the first report describing surfactin production by B. subtilis using stearin and soap wastes as carbon sources. A complete chemical characterization of surfactin analogs produced from the different waste glycerol samples was performed by liquid chromatography–mass spectrometry (LC-MS) and Fourier transform infrared spectroscopy (FTIR). Furthermore, the surfactin produced in the study exhibited good stability in a wide range of pH, salinity and temperatures, suggesting its potential for several applications in biotechnology.

Induction of Systemic Resistance in Maize and Antibiofilm Activity of Surfactin From Bacillus velezensis MS20

“…In the present study, the PCR amplification of surfactin gene gave strong band at 675 bp which is similar to reports by Swapna et al (2016) . FTIR spectra revealed the presence of functional groups which are characteristic of peptides and aliphatic chains found in surfactin lipopeptide, and LC/ESI-MS peaks showed isoforms normally observed for surfactin, i.e., C12-C16 vibrations in positive and negative polarities which are in accordance with the reports of Table 2 (Sarwar et al, 2018 ; Janek et al, 2021 ).…”

Corner flows induced by surfactant-producing bacteria Bacillus subtilis and Pseudomonas fluorescens

“…Next, we investigate the concentration of surfactants produced by bacteria that can generate corner flows. Specifically, we repeat the corner flow experiments using various concentrations of commercially-available surfactants rhamnolipid (Sigma) and surfactin (Sigma), which are the surfactants produced by P. fluorescens 31,32 and B. subtilis 33,34 , respectively. Here, we measured the surface tension and contact angle of surfactant solutions at different concentrations and transferred surfactant solutions into chambers to observe the rise of corner flow at the 30° corner.…”

A new bacterial-derived biosurfactant for biotechnological applications in the oil industry: production, optimization, biosurfactant functional and physicochemical characterization

“…By way of comparison, we note, for example, that the MI27-derived BioS is more effective than surfactin isoforms produced by Bacillus subtilis isolate LSFM-05 (de Faria et al 2011), those produced by B. subtilis SL having a CMC value of 154 mg/L and a ST of 28.68 mN/m (Wu et al 2022) and surfactin isoforms produced by Bacillus nealsonii S2MT (Phulpoto et al 2020). In means of the ST reduction power, it is more efficient than surfactin isoforms produced by B. subtilis BS-37 displaying a CMC value of mg/L (Liu et al 2014), Bacillus amyloliquefaciens MO13-derived surfactin isoforms displaying a CMC value of 36 mg/L and an ST of 27 mN/m (Moro et al 2022), engineered surfactin isoforms produced by B. subtilis 168 having a CMC value of 38-36 mg/L with potential ST of 28 mN/m (Hu et al 2021) and surfactin isoforms derived from B. subtilis #309 having a CMC value of 15 mg/L with potential ST of 28 mN/m (Janek et al 2021).…”