Suscetibilidade antimicrobiana entre amostras de Enterococcus isoladas no Hospital Universitário de Santa Maria

In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital.

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“…This high susceptibility rate to ampicillin is similar to rates from other studies (7,13,16) and confirm that E.…”

Section: Discussionsupporting

confidence: 89%

Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil

Bender¹,

Freitas²,

Reiter³

et al. 2009

Braz. J. Microbiol.

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“…Due to Enterococcus spp. resistance to antimicrobials such as penicillin, ampicillin, aminoglycosides, tetracycline, and vancomycin and its association with nosocomial infections[ 24 ], it is crucial to identify these bacteria in different types of biological samples, including HM.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of resistance and pathogenicity of Enterococcus spp. in samples of donor breast milk

Santana

Andrade

Silva

et al. 2020

WJCP

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“…Enterococcus faecalis is one of the main nosocomial pathogens ( Horner et al , 2005 ) which may be resistant to a wide range of antimicrobial agents, causing several diseases. These bacteria inhabit the gastrointestinal and genitourinary tracts and have been related to infectious endocarditis and surgical wound infection ( Pasticci et al , 2008 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of Amazon plant extracts against Enterococcus faecalis

Castilho¹,

Silva²,

Saraceni³

et al. 2014

Braz. J. Microbiol.

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Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests’ plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC® 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by 13C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.

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Suscetibilidade antimicrobiana entre amostras de Enterococcus isoladas no Hospital Universitário de Santa Maria

Cited by 8 publications

References 13 publications

Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil

Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil

Identification and characterization of resistance and pathogenicity of Enterococcus spp. in samples of donor breast milk

In vitro activity of Amazon plant extracts against Enterococcus faecalis

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