Identification and characterization of resistance and pathogenicity of <i>Enterococcus spp</i>. in samples of donor breast milk

Santana, Luana Andrade Mendes; Andrade, Nívea Nara Novais; Silva, Lucas Santana Coelho da; Oliveira, Caline Novais Teixeira; Brito, Breno Bittencourt de; Melo, Fabrício Freire de; Souza, Cláudio Lima; Marques, Lucas Miranda; Oliveira, Márcio Vasconcelos

doi:10.5409/wjcp.v9.i3.53

Cited by 2 publications

(1 citation statement)

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“…All breast milk isolates carry genes for virulence factors, and four of them (E. faecalis BM5, BM6, BM9, and BM10) contain seven out of the eight screened virulence genes. Our results differ from those reported by Santana et al [73], who investigated the distribution of ace, efaA, gelE, cylA, hyl, and esp virulence genes among an enterococcal population isolated from raw human breast milk. In their investigation, only two genes were detected (efaA and ace).…”

Section: Discussioncontrasting

confidence: 99%

Pathogenic Potential and Antibiotic Susceptibility: A Comprehensive Study of Enterococci from Different Ecological Settings

Pandova,

Kizheva,

Tsenova

et al. 2023

Pathogens

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The pathway and the lifestyle of known enterococcus species are too complicated. The aim of the present study is to trace the path of pathogenicity of enterococci isolated from seven habitats (Cornu aspersum intestine; Bulgarian yoghurt; goat and cow feta cheese—mature and young, respectively; Arabian street food—doner kebab; cow milk; and human breast milk) by comparing their pathogenic potential. In total, 72 enterococcal strains were isolated and identified by MALDI-TOF, sequencing, and PCR. Hemolytic and gelatinase activity were biochemically determined. PCR was carried out for detection of virulence factors (cylB, esp, gls24, nucl, psaA, agg, gelE, and ace) and antibiotic resistance (erm, ermB, blaZ, vanA, aphA, mefA, gyrA, catpIP501, and aac6′-aph2″). Phenotypic antibiotic resistance was assigned according to EUCAST. Eleven representatives of the genus Enterococcus were identified: E. mundtii, E. casseliflavus, E. gilvus, E. pseudoavium, E. pallens, E. malodoratus, E. devriesei, E. gallinarum, E. durans, E. faecium, and E. faecalis. Twenty-two strains expressed α-hemolysis. Thirteen strains had the cylB gene. Only two strains expressed α-hemolysis and possessed the cylB gene simultaneously. Positive amplification for gelE was found in 35% of the isolates, but phenotypic gelatinase activity was observed only in three strains. All isolates showed varying antibiotic resistance. Only E. faecalis BM15 showed multiple resistance (AMP-HLSR-RP). Correlation between genotypic and phenotypic macrolide resistance was revealed for two E. faecalis strains.

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Section: Discussioncontrasting

confidence: 99%