Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni

Inoue, Kota; Tsujio, Masashi; Matsubayashi, Makoto; Inoue, Ryota; Hatai, Hitoshi; Andoh, Masako; Abé, Keisuke; Matsui, Toshihiro; Matsuo, Tomohide

doi:10.2478/s11686-019-00052-w

Cited by 2 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intestinal coccidiosis is an economically important disease of domestic animals such as poultry, cattle, sheep, and pigs [ 16 , 31 ]. In this study, oocyte excretion of E. vermiformis was completely prevented when mice were treated with a 15 mg/kg dose of TTZ, which is the recommended dose for cattle [ 12 ], even though it has been reported that E. krijgsmanni , murine Eimeria , showed resistance to TTZ treatment at the same dose as that used in our study [ 8 , 34 ]. Our findings showed that the effects of DCZ were dose-dependent over the range of doses tested (0.2, 1, 5, 15, or 45 mg/kg), and 45 mg/kg of DCZ was the most effective dose for reducing oocyst excretion.…”

Section: Discussionmentioning

confidence: 69%

Toltrazuril and diclazuril: comparative evaluation of anti-coccidial drugs using a murine model

Ahmadi

BAAKHTARI

Yasuda

et al. 2022

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Intestinal coccidiosis caused by Eimeria protozoan species is an economically important disease, especially in poultry and cattle. Anti-coccidial drugs commonly used for controlling coccidiosis are toltrazuril (TTZ) and diclazuril (DCZ). In this study, the efficacies of TTZ and DCZ were compared using a murine model, and the effect of these treatments on the induction of acquired resistance was evaluated. Male C57BL/6J mice were inoculated with 1,000 sporulated E. vermiformis oocytes and treated with TTZ or DCZ. The recommended TTZ dose for cattle (15 mg/kg) completely prevented oocyte excretion. But, mice required 5 mg/kg of DCZ, which is five times the recommended dose for cattle, to reduce oocyte excretion. In E. vermiformis re-infection, TTZ (15 mg/kg) and DCZ (5 mg/kg) treatments did not interfere with the development of acquired resistance. Bodyweight gain was significantly higher in the TTZtreated group than in the control (untreated/infected) group and the DCZ-treated group, and no significant difference in bodyweight gain was observed between the TTZ-treated group and the healthy (uninfected/untreated) group. Analysis of T lymphocyte subsets in the spleen and mesenteric lymph nodes indicated that the relative populations of CD4 + and CD8 + T cells were reduced in the DCZ-treated and control (untreated/infected) groups, suggesting there was immunosuppression during the infection. However, no reductions in T cell populations were observed in the TTZ-treated group. The results indicated that an optimal anti-coccidial drug is one that can completely break the parasite life cycle in the host animal.

show abstract

Section: Discussionmentioning

confidence: 69%

Toltrazuril and diclazuril: comparative evaluation of anti-coccidial drugs using a murine model

Ahmadi

BAAKHTARI

Yasuda

et al. 2022

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

Potential Development Ability of Residual Zoites, a Second-Generation Meront, Inducing Long-Term Infection by the Mouse Eimerian Parasite, Eimeria krijgsmanni

Mizuno,

Kiyotake,

Matsubayashi

et al. 2024

Acta Parasit.

View full text Add to dashboard Cite

Purpose Coccidiosis caused by eimerian parasites results in lethal watery or bloody diarrhea in hosts, and markedly impairs the growth of and feed utilization by host animals. We previously investigated detailed the life cycle of Eimeria krijgsmanni as a mouse eimerian parasite. Only second-generation meronts, as an asexual stage, were morphologically detected in the epithelium of the host cecum for at least 8 weeks after infection, even though oocyst shedding finished approximately 3 weeks after infection. The presence of zoites was of interest because infection by eimerian parasites is considered to be self-limited after their patent period. Methods To clarify the significance of residual second-generation meronts in E. krijgsmanni infection, we performed infection experiments using immunocompetent mice under artificial immunosuppression and congenital immunodeficient mice. Results The number of oocysts discharged and the duration of oocyst discharge both increased in immunosuppressed mice. In immunodeficient mice, numerous oocysts were shed over a markedly longer period, and oocyst discharge did not finish until 56 days after inoculation. Conclusions The present results suggest that the second-generation meronts survived in the epithelial cells of the cecum after the patent period, thereby contributing to extended infection as an asexual stage. The results obtained on E. krijgsmanni indicate that infections by Eimeria spp. are not self-limited and potentially continue for a long period of time.

show abstract

Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni

Cited by 2 publications

References 28 publications

Toltrazuril and diclazuril: comparative evaluation of anti-coccidial drugs using a murine model

Toltrazuril and diclazuril: comparative evaluation of anti-coccidial drugs using a murine model

Potential Development Ability of Residual Zoites, a Second-Generation Meront, Inducing Long-Term Infection by the Mouse Eimerian Parasite, Eimeria krijgsmanni

Contact Info

Product

Resources

About