Susceptibility to insecticides of Anopheles darlingi Root 1840, in two locations of the departments of Santander and Caquetá, Colombia

Santacoloma, Liliana; Tibaduiza, Tania; Gutiérrrez, Marcela; Brochero, Helena

doi:10.7705/biomedica.v32i0.455

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…An. darlingi collected in Antioquia, Chocó, Putumayo and Santander showed resistance to alpha-cypermethrin, deltamethrin, lambda-cyhalothrin, permethrin, fenitrothion, propoxur, and DDT [7, 9, 11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Insecticide Resistance and Its Intensity in Populations of Malaria Vectors in Colombia

Orjuela

Morales

Ahumada

et al. 2018

BioMed Research International

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Insecticide resistance in malaria vectors threatens malaria prevention and control efforts. In Colombia the three primary vectors, Anopheles darlingi, An. nuneztovari s.l., and An. albimanus, have reported insecticide resistance to pyrethroids, organophosphates, carbamates, and DDT; however, the insecticide resistance monitoring is not continuous, and the data on the prevalence of resistance is scarce and geographically limited. We describe the resistance levels and intensity of previously detected resistant populations among primary malaria vectors from the most endemic malaria areas in Colombia. The study was carried out in 10 localities of five states in Colombia. Bioassays were carried out following the methodology of CDC Bottle Bioassay using the discriminating concentration and in order to quantify the intensity the specimens were exposed to 2, 5, and 10X discriminating concentrations. Five insecticides were tested: deltamethrin, lambda-cyhalothrin, alpha-cypermethrin, permethrin, and DDT. The results provide evidence of low resistance intensity and resistance highly localized to pyrethroids and DDT in key malaria vectors in Colombia. This may not pose a threat to malaria control yet but frequent monitoring is needed to follow the evolution of insecticide resistance.

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Section: Introductionmentioning

confidence: 99%

Insecticide Resistance and Its Intensity in Populations of Malaria Vectors in Colombia

Orjuela

Morales

Ahumada

et al. 2018

BioMed Research International

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Ecology of Anopheles darlingi, the Primary Malaria Vector in the Americas and Current Nongenetic Methods of Vector Control

Conn

Ribolla

2016

Genetic Control of Malaria and Dengue

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Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

Orjuela

Álvarez-Díaz

Morales

et al. 2019

Malar J

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BackgroundKnockdown resistance (kdr) is a well-characterized target-site insecticide resistance mechanism that is associated with DDT and pyrethroid resistance. Even though insecticide resistance to pyrethroids and DDT have been reported in Anopheles albimanus, Anopheles benarrochi sensu lato (s.l.), Anopheles darlingi, Anopheles nuneztovari s.l., and Anopheles pseudopunctipennis s.l. malaria vectors in Latin America, there is a knowledge gap on the role that kdr resistance mechanisms play in this resistance. The aim of this study was to establish the role that kdr mechanisms play in pyrethroid and DDT resistance in the main malaria vectors in Colombia, in addition to previously reported metabolic resistance mechanisms, such as mixed function oxidases (MFO) and nonspecific esterases (NSE) enzyme families.MethodsSurviving (n = 62) and dead (n = 67) An. nuneztovari s.l., An. darlingi and An. albimanus mosquitoes exposed to diagnostic concentrations of DDT and pyrethroid insecticides were used to amplify and sequence a ~ 225 bp fragment of the voltage-gated sodium channels (VGSC) gene. This fragment spanning codons 1010, 1013 and 1014 at the S6 segment of domain II to identify point mutations, which have been associated with insecticide resistance in different species of Anopheles malaria vectors.ResultsNo kdr mutations were detected in the coding sequence of this fragment in 129 samples, 62 surviving mosquitoes and 67 dead mosquitoes, of An. darlingi, An. nuneztovari s.l. and An. albimanus.ConclusionMutations in the VGSC gene, most frequently reported in other species of the genus Anopheles resistant to pyrethroid and DDT, are not associated with the low-intensity resistance detected to these insecticides in some populations of the main malaria vectors in Colombia. These results suggest that metabolic resistance mechanisms previously reported in these populations might be responsible for the resistance observed.

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Susceptibility to insecticides of Anopheles darlingi Root 1840, in two locations of the departments of Santander and Caquetá, Colombia

Cited by 5 publications

References 10 publications

Insecticide Resistance and Its Intensity in Populations of Malaria Vectors in Colombia

Insecticide Resistance and Its Intensity in Populations of Malaria Vectors in Colombia

Ecology of Anopheles darlingi, the Primary Malaria Vector in the Americas and Current Nongenetic Methods of Vector Control

Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

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