Susceptibility testing of tulathromycin: Interpretative breakpoints and susceptibility of field isolates

Godinho, Kevin

doi:10.1016/j.vetmic.2007.11.033

Cited by 48 publications

(36 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isolates of M. haemolytica ( N = 4,548) were screened for tulathromycin susceptibility by plating onto BHI plates supplemented with 2 μg/mL tulathromycin, followed by incubation at 37°C for 24 h. A concentration of 2°μg/mL was selected based on a previous study reporting 97% of bovine M. haemolytica field isolates having tulathromycin minimum inhibitory concentrations (MICs) of ≤2 μg/mL (Godinho, 2008). Tulathromycin was kindly provided by Pfizer Animal Health (now Zoetis) and prepared according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Macrolides consist of a core macrolactone ring that binds to the large subunit of the ribosome and blocks bacterial protein synthesis (Poehlsgaard and Douthwaite, 2005). Differences in the ring structure can affect pharmacokinetic/pharmacodynamic properties and have been the basis for the development of new macrolide derivatives over the years (Godinho, 2008). Tulathromycin is a macrolide that has three amine rings and is further sub-classified as a triamilide.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

et al. 2013

View full text Add to dashboard Cite

Mannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a 3-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814 cattle were collected when cattle entered feedlots (N = 4) and again from the same cattle after ≥60 days on feed. The antimicrobial use history for each animal was recorded. Mannheimia haemolytica was isolated from 796 (13.7%) entry samples and 1,038 (20.6%) ≥ 60 days samples. Of the cattle positive for M. haemolytica, 18.5, 2.9, and 2.4% were administered therapeutic concentrations of tulathromycin, tilmicosin, or tylosin tartrate, respectively. In addition, 13.2% were administered subtherapeutic concentrations of tylosin phosphate in feed. In years one and two, no tulathromycin-resistant M. haemolytica were detected, whereas five isolates (0.4%) were resistant in year three. These resistant isolates were collected from three cattle originating from a single pen, were all serotype 1, and were genetically related (≥89% similarity) according to pulsed-field gel electrophoreses patterns. The five tulathromycin-resistant isolates were multi-drug resistant also exhibiting resistance to oxytetracycline, tilmicosin, ampicillin, or penicillin. The macrolide resistance genes erm(42), erm(A), erm(B), erm(F), erm(X) and msr(E)-mph(E), were not detected in the tulathromycin-resistant M. haemolytica. This study showed that tulathromycin resistance in M. haemolytica from a general population of feedlot cattle in western Canada was low and did not change over a 3-year period after tulathromycin was approved for use in cattle.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Therefore, a modified method of MIC determination and recommended breakpoints for veterinary fastidious microorganisms (Actinobacillus pleuropneumoniae and Histophilus somni) was used according to CLSI (2008). Nevertheless, the interpretive criteria taken from a proposal of clinical breakpoints for amoxicillin (Schwarz et al 2010) were derived for ampicillin and amoxicillin/clavulanic acid and interpretative breakpoints for tulathromycin (Godinho 2008) were accepted. The susceptibility ranges of P. multocida and H. parasuis were recorded along with the MIC that inhibited 50% (MIC 50 ) and 90% (MIC 90 ) of the isolates.…”

mentioning

confidence: 99%

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Nedbalcová

Kučerová

2013

Acta Vet. Brno

View full text Add to dashboard Cite

Pasteurella multocida and Haemophilus parasuis pig isolates obtained in the Czech Republic were tested for their susceptibility against selected antimicrobial agents by broth microdilution method between 2008 and 2011. A low degree of resistance was observed for ampicillin, amoxicillin/clavulanic acid, ceftiofur, tulathromycin, tilmicosin, florfenicol and enrofloxacin in 20 (6.0%), 15 (4.5 %), 2 (0.6%), 8 (2.4%), 13 (3.9%), 5 (1.5%) and 5 (1.5%) P. multocida isolates as well as for tiamulin, gentamicin, tulathromycin, tilmicosin and ampicillin in 2 (2.4%), 2 (2.4%), 3 (3.6%), 3 (3.6%) and 6 (7.2%) H. parasuis isolates. In addition, moderate level of resistance to tiamulin was found in 60 (18.1%) P. multocida isolates and high level of resistance for tetracycline was detected in 107 (32.2 %) P. multocida isolates and in 23 (27.7 %) H. parasuis isolates. Differences between resistance rates of P. multocida and H. parasuis were significant (P ≤ 0.5) only for tiamulin. These data confirmed that antimicrobial resistance is not very widespread among current porcine P. multocida and H. parasuis isolates in the Czech Republic. Antimicrobial resistance, minimal inhibition concentration, respiratory diseases, pigsPasteurella multocida and Haemophilus parasuis are the causative agents of infectious diseases of porcine respiratory tract which cause major economic losses by their negative impact on the weight gain, feed conversion, and health status (Oliveira and Pijoan 2004;Shin et al. 2005). Antibiotic treatment is one of the commonly used measures for the control of pasteurellosis and H. parasuis infections but the use of antimicrobial agents leads to both selection and increase of resistance (Schwarz and Chaslus-Dancla 2001). Failure to treat infectious disease caused by resistant bacteria leads to increased morbidity and mortality (Kolar et al. 2010). Variations in the antimicrobial use for the control of bacterial pathogens in pigs from one country to another can contribute to evident differences in antimicrobial susceptibility of P. multocida and H. parasuis. In accordance with the recommendation of Schwarz et al. (2010) on the requirement of application of the same methodology and interpretive criteria (which would allow for re-evaluation of the original data if the interpretive criteria change over time), only a limited number of recent studies were conducted regarding the susceptibility of P. multocida and H. parasuis to antimicrobials with the use of microdilution method.Considering the lack of recent studies of antimicrobial susceptibility testing of P. multocida and H. parasuis within the European territory, the aim of this work was to examine a panel of various isolates obtained from different Czech locations to determine their susceptibility to selected antimicrobial agents by the broth microdilution method, in accordance with the guidelines issued by the Clinical and Laboratory Standards Institute (CLSI) in 2008. Materials and Methods SamplingAll P. multocida and H. parasuis isolates were obtained fro...

show abstract

“…Antimicrobial agents are commonly applied to control respiratory tract infections in cattle (9,14). Based on the available susceptibility data, most bovine P. multocida strains are still susceptible to newer antimicrobial agents, including the 15-membered ring macrolide tulathromycin (4,7,16). Since tulathromycin has a unique chemical structure characterized by the presence of three amine groups, it has been referred to in the literature as the first representative of the triamilide subclass among the macrolides (3).…”

mentioning

confidence: 99%

Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

Kadlec

Michael

Sweeney

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The mechanism of macrolide-triamilide resistance in Pasteurella multocida has been unknown. During whole-genome sequencing of a multiresistant bovine P. multocida isolate, three new resistance genes, the rRNA methylase gene erm(42), the macrolide transporter gene msr(E), and the macrolide phosphotransferase gene mph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)-mph(E)] in macrolide-susceptible Escherichia coli and P. multocida hosts.

show abstract

Susceptibility testing of tulathromycin: Interpretative breakpoints and susceptibility of field isolates

Abstract: Susceptibility testing of tulathromycin: interpretative breakpoints and susceptibility of field isolates.

Cited by 48 publications

References 4 publications

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

Contact Info

Product

Resources

About