Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

Kadlec, Kristina; Michael, Geovana Brenner; Sweeney, Michael T.; Brzuszkiewicz, Elżbieta; Liesegang, Heiko; Daniel, Rolf; Watts, Jeffrey L.; Schwarz, Štefan

doi:10.1128/aac.00092-11

Cited by 57 publications

(44 citation statements)

References 14 publications

(11 reference statements)

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“…Higher consumption of amfenicols and cephalosporines at tested P. multocida and H. parasuis strains did not manifested by an increase in the level of resistance, which remained within the range from undetected to low levels. Lincosamides were not tested in this study, but even though cross-resistance is known among macrolide, triamilide and lincosamide (Kadlec et al 2011), the level of resistance to tested triamilide (tulathromycin) and macrolide (tilmicosin) remained at a low level.…”

Section: Discussionmentioning

confidence: 94%

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Nedbalcová

Kučerová

2013

Acta Vet. Brno

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Pasteurella multocida and Haemophilus parasuis pig isolates obtained in the Czech Republic were tested for their susceptibility against selected antimicrobial agents by broth microdilution method between 2008 and 2011. A low degree of resistance was observed for ampicillin, amoxicillin/clavulanic acid, ceftiofur, tulathromycin, tilmicosin, florfenicol and enrofloxacin in 20 (6.0%), 15 (4.5 %), 2 (0.6%), 8 (2.4%), 13 (3.9%), 5 (1.5%) and 5 (1.5%) P. multocida isolates as well as for tiamulin, gentamicin, tulathromycin, tilmicosin and ampicillin in 2 (2.4%), 2 (2.4%), 3 (3.6%), 3 (3.6%) and 6 (7.2%) H. parasuis isolates. In addition, moderate level of resistance to tiamulin was found in 60 (18.1%) P. multocida isolates and high level of resistance for tetracycline was detected in 107 (32.2 %) P. multocida isolates and in 23 (27.7 %) H. parasuis isolates. Differences between resistance rates of P. multocida and H. parasuis were significant (P ≤ 0.5) only for tiamulin. These data confirmed that antimicrobial resistance is not very widespread among current porcine P. multocida and H. parasuis isolates in the Czech Republic. Antimicrobial resistance, minimal inhibition concentration, respiratory diseases, pigsPasteurella multocida and Haemophilus parasuis are the causative agents of infectious diseases of porcine respiratory tract which cause major economic losses by their negative impact on the weight gain, feed conversion, and health status (Oliveira and Pijoan 2004;Shin et al. 2005). Antibiotic treatment is one of the commonly used measures for the control of pasteurellosis and H. parasuis infections but the use of antimicrobial agents leads to both selection and increase of resistance (Schwarz and Chaslus-Dancla 2001). Failure to treat infectious disease caused by resistant bacteria leads to increased morbidity and mortality (Kolar et al. 2010). Variations in the antimicrobial use for the control of bacterial pathogens in pigs from one country to another can contribute to evident differences in antimicrobial susceptibility of P. multocida and H. parasuis. In accordance with the recommendation of Schwarz et al. (2010) on the requirement of application of the same methodology and interpretive criteria (which would allow for re-evaluation of the original data if the interpretive criteria change over time), only a limited number of recent studies were conducted regarding the susceptibility of P. multocida and H. parasuis to antimicrobials with the use of microdilution method.Considering the lack of recent studies of antimicrobial susceptibility testing of P. multocida and H. parasuis within the European territory, the aim of this work was to examine a panel of various isolates obtained from different Czech locations to determine their susceptibility to selected antimicrobial agents by the broth microdilution method, in accordance with the guidelines issued by the Clinical and Laboratory Standards Institute (CLSI) in 2008. Materials and Methods SamplingAll P. multocida and H. parasuis isolates were obtained fro...

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Section: Discussionmentioning

confidence: 94%

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Nedbalcová

Kučerová

2013

Acta Vet. Brno

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“…For example, genes encoding multiple antibiotic resistances have been identified integrated into the chromosome of the genome-sequenced P. multocida strain 36950 isolated from a case of BRD that exhibited resistance to all the antibiotics commonly used to control BRD (80,537). Interestingly, the 85-kb ICEPmu1 element carried 88 genes, including 12 different resistance genes that were distributed between two regions of resistance (80), a 15.7-kb region at one end of the element and a 9.8-kb region at the other end.…”

Section: Antibiotic Resistancementioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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“…During the last few years, however, strains of M. haemolytica and P. multocida that are resistant to several veterinary antibiotics have appeared, and most recent reports have included strains resistant to macrolides (9,10,14,19). The resistance determinants have been well documented for most of the drugs (9), although the macrolide resistance mechanisms (3,4,8) and the means of dissemination of the resistance determinants (10) have only just been characterized.…”

mentioning

confidence: 99%

“…The class 2 isolates contain two resistance genes, msr(E) and mph(E), which are arranged in tandem and expressed from the same promoter, and encode a macrolide efflux pump and a macrolide-inactivating phosphotransferase, respectively (4). A third class of isolates is highly resistant to a comprehensive set of macrolides and contains all three determinants, erm(42), msr(E), and mph(E) (4,8).…”

mentioning

confidence: 99%

Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida

Rose

Desmolaize

Jaju

et al. 2012

Antimicrob Agents Chemother

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bThe bacterial pathogens Mannheimia haemolytica and Pasteurella multocida are major etiological agents in respiratory tract infections of cattle. Although these infections can generally be successfully treated with veterinary macrolide antibiotics, a few recent isolates have shown resistance to these drugs. Macrolide resistance in members of the family Pasteurellaceae is conferred by combinations of at least three genes: erm(42), which encodes a monomethyltransferase and confers a type I MLS B (macrolide, lincosamide, and streptogramin B) phenotype; msr(E), which encodes a macrolide efflux pump; and mph(E), which encodes a macrolide-inactivating phosphotransferase. Here, we describe a multiplex PCR assay that detects the presence of erm(42), msr(E), and mph(E) and differentiates between these genes. In addition, the assay distinguishes P. multocida from M. haemolytica by amplifying distinctive fragments of the 23S rRNA (rrl) genes. One rrl fragment acts as a general indicator of gammaproteobacterial species and confirms whether the PCR assay has functioned as intended on strains that are negative for erm(42), msr(E), and mph(E). The multiplex system has been tested on more than 40 selected isolates of P. multocida and M. haemolytica and correlated with MICs for the veterinary macrolides tulathromycin and tilmicosin, and the newer compounds gamithromycin and tildipirosin. The multiplex PCR system gives a rapid and robustly accurate determination of macrolide resistance genotypes and bacterial genus, matching results from microbiological methods and whole-genome sequencing.

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Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

Cited by 57 publications

References 14 publications

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Antimicrobial susceptibility of Pasteurella multocida and Haemophilus parasuis isolates associated with porcine pneumonia

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida

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