Susceptibility of Mammalian Erythrocytes to Hemolysis With Hypotonic Solutions

Castle, W. E.; Daland, Geneva A.

doi:10.1001/archinte.1937.00180060002001

Cited by 38 publications

(6 citation statements)

References 13 publications

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“…An excellent positive correlation was also found between the amount of cholesterol per pmz erythrocyte surface area and the amount of cholesterol per cell ( Fig 5). Castle & Daland (1937) established that the susceptibility of erythrocytes to hypotonic haemolysis can be regarded as a function of the percentage difference between the original discoidal volume and the volume of a sphere of equal surface and that the difference is proportionate to the ratio MCD : MCT. In agreement with this we found a negative correlation between MCD:MCT and 50% haemolysis (correlation coefficient: 0.79).…”

Section: Relation Between Macroplania and Leptocytosis Diminished Osmentioning

confidence: 99%

“…In agreement with this we found a negative correlation between MCD:MCT and 50% haemolysis (correlation coefficient: 0.79). It has been demonstrated that osmotic fragility is influenced much more by the MCD:MCT ratio than by the mean erythrocyte surface area (Castle & Daland, 1937) and the mean amount of cholesterol per pm2 erythrocyte surface arca (Werre, 1968).…”

Section: Relation Between Macroplania and Leptocytosis Diminished Osmentioning

confidence: 99%

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Causes of Macroplania of Erythrocytes in Diseases of the Liver and Biliary Tract with Special Reference to Leptocytosis

et al. 1970

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Summary In diseases of the liver or biliary tract increased surface area (macroplania) is related either to macrocytosis or to leptocytosis. In a study on 20 patients with macroplania associated with leptocytosis, but without target cells, the following observations were made. There was a positive correlation between changes in MCD and serum bilirubin. There was a negative correlation between MCD and osmotic fragility. There was a positive correlation between MCD and mean amount of cholesterol per erythrocyte and per μm2 of erythrocyte surface area, but there was no correlation between mean amount of cholesterol per erythrocyte and serum concentration of free or total cholesterol. The osmotic fragility of normal erythrocytes introduced into the circulation of patients with leptocytosis became similar to the (diminished) osmotic fragility of the patients' own erythrocytes. The order in which the changes occur is probably as follows: changes in the peripheral circulation→increase in the mean amount of cholesterol per erythrocyte and per unit of erythrocyte surface area→increase in mean erythrocyte surface area while the MCV remains normal→increase of MCD and diminution of MCT→diminution of osmotic fragility.

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Section: Relation Between Macroplania and Leptocytosis Diminished Osmentioning

confidence: 99%

Section: Relation Between Macroplania and Leptocytosis Diminished Osmentioning

confidence: 99%

Causes of Macroplania of Erythrocytes in Diseases of the Liver and Biliary Tract with Special Reference to Leptocytosis

et al. 1970

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“…The "osmotic fragility" of a red cell is in essence a measure of the difference between its (isotonic) volume in the blood and its volume as a sphere of equal surface at the moment of rupture in hypotonic saline (48). The amounts of he-*Hemoglobin production was calculated by means of a simplified planimetric method which is applicable to nonhomeostatic states, and in which hemoglobin turnover is derived from red cell survival and blood volume data.…”

Section: Red Cell Survivalmentioning

confidence: 99%

The Anemia of Liver Disease: Observations on Its Mechanism 1

Jandl¹

1955

J. Clin. Invest.

230

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“…Many studies have demonstrated the relationship between altered osmotic fragility and abnormal geometry of erythrocytes [1,11,21]. Elevated osmotic fragility, however, is not only important with respect to a long life-span of erythrocyte carriers and the encapsulated drugs, but also with respect to an uncontrolled breakdown of loaded cells in vitro and a reduced shelf-life.…”

Section: Discussionmentioning

confidence: 99%

Osmotic fragility of drug carrier erythrocytes

Sprandel¹,

Zöllner²

1985

Res. Exp. Med.

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Erythrocytes have been proposed as biogradable cellular carriers for drugs. Potentials of this therapeutic approach are organ-specific targeting, protection and prolonged in vivo function of the encapsulated drugs. Previous studies demonstrated the advantage of a hypo-osmotic dialysis procedure for macromolecule loading resulting in cells that are closely similar to normal erythrocytes. Osmotic fragility of unloaded and loaded "carrier" erythrocytes was studied both in respect of shelf-life and in vivo survival. Sudden haemolysis which is characteristic for normal erythrocytes was never obtained with carrier erythrocytes. Haemolysis appeared at all osmotic pressures and increased stepwise indicating the existence of various cell populations. However, the majority of cells were haemolysed at lower values of the osmotic fragility curve. Osmotic fragility was highly increased when cytotoxic chemotherapeutics were encapsulated, and these cells appeared as spherocytes using scanning electron microscopy. Osmotic fragility proved to be a simple but reliable method for the in vitro evaluation of carrier erythrocytes and the effect of the encapsulated substances.

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Susceptibility of Mammalian Erythrocytes to Hemolysis With Hypotonic Solutions

Cited by 38 publications

References 13 publications

Causes of Macroplania of Erythrocytes in Diseases of the Liver and Biliary Tract with Special Reference to Leptocytosis

Causes of Macroplania of Erythrocytes in Diseases of the Liver and Biliary Tract with Special Reference to Leptocytosis

The Anemia of Liver Disease: Observations on Its Mechanism 1

Osmotic fragility of drug carrier erythrocytes

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