Susceptibility of Listeria monocytogenes isolated from food in Italy to antibiotics

Aureli, Paolo; Ferrini, Anna Maria; Mannoni, Veruscka; Hodzic, Snjezana; Wedell-Weergaard, Christina; Oliva, B

doi:10.1016/s0168-1605(02)00381-1

Cited by 51 publications

(32 citation statements)

References 24 publications

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“…The MIC values of streptomycin against L. monocytogenes strains were equal or higher than the generally accepted Soussy's MIC breakpoint values, ranging from 8 to 16 µg/mL (Aureli et al, 2003). In contrast, the MIC values of gentamycin against L. monocytogenes strains, with the exception of the type strain KCTC 3710, were lower than the generally accepted Soussy's MIC breakpoint values, ranging from 4 to 8 µg/mL (Aureli et al, 2003). These results indicate that streptomycin is no longer useful for treating Listeria infections.…”

Section: Antibiotic Resistance Of L Monocytogenes Strainsmentioning

confidence: 64%

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Nshimiyumukiza¹,

Kang²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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In an effort to discover alternative antimicrobials against Listeria monocytogenes, several marine algae were screened. The methanolic extract of Ecklonia cava exhibited the highest antibacterial activity against L. monocytogenes, with the ethyl acetate (EtOAc) soluble fraction of E. cava methanolic extract having a MIC value of 256 µg/mL and a MBC value of 512 µg/mL. The MIC values of streptomycin in combination with the EtOAc fraction were markedly reduced up to 64-fold, suggesting that the antibacterial activity of the antibiotic was restored when combined with the EtOAc fraction. The interaction between streptomycin and the EtOAc fraction was assessed by fractional inhibitory concentration (FIC) indices. The combination of streptomycin and the EtOAc fraction against L. monocytogenes resulted in ∑FIC min range of 0.141 to 0.266 and ∑FIC max of 0.531 for all strains. The median ∑FIC against L. monocytogenes strains ranged from 0.172 to 0.344. Thus, synergistic ranges of FIC <1 were observed for all combinations of streptomycin and the EtOAc fraction against L. monocytogenes strains. Indeed, the median ∑FIC of streptomycin-EtOAc fraction ranged from 0.172 to 0.344, suggesting a marked synergy.

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Section: Antibiotic Resistance Of L Monocytogenes Strainsmentioning

confidence: 64%

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Nshimiyumukiza¹,

Kang²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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“…3 Os antimicrobianos mais indicados no tratamento da listeriose são : tetraciclina, coumercina, rifampicina, cloranfenicol, eritromicina e ampicilina, sendo esta última adicionada de um antibiótico aminoglicosídio. 4,5 Marth 6 relata que a maioria das cepas é sensível à tetraciclina, cloranfenicol, eritromicina e cefalotina.…”

Section: Introductionunclassified

Resistência antimicrobiana de bactérias do gênero Listeria spp: isoladas de carne moída bovina

Mantilla

Franco

Oliveira

et al. 2008

Braz. J. Vet. Res. Anim. Sci.

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ResumoCom o aumento do uso de antimicrobianos como promotores de crescimento e até mesmo com fins terapêuticos na criação de animais de produção, existe o interesse global referente ao consumo de baixos níveis de resíduos de antimicrobianos em alimentos e os efeitos destes na saúde humana. A ingestão de alimentos contendo resíduos de fármacos antimicrobianos pode ocasionar resistência bacteriana aos antimicrobianos utilizados rotineiramente na terapêutica humana, dificultando o tratamento de enfermidades infecciosas humanas. Neste experimento foi realizado o teste de sensibilidade aos antimicrobianos em cepas de Listeria spp. isoladas a partir de amostras de carnes bovinas, através da metodologia descrita pelo NCCLS em 2003. De acordo com os resultados, todas as cepas isoladas foram resistentes à maioria dos antimicrobianos testados. As cepas de L. monocytogenes foram resistentes à gentamicina, cefoxitina, ampicilina, clindamicina, oxaciclina e sulfazotrim. As cepas de L. innocua isoladas foram resitentes à gentamicina, cefoxitina, tetraciclina, vancomicina, oxaciclina e clindamicina. Palavras-chave:Antimicrobianos. Alimentos. Listeriose. IntroduçãoA utilização de antimicrobianos gerou grande otimismo em relação à prevenção e ao tratamento dos processos infecciosos. Entretanto, o uso exagerado e nem sempre criterioso ou racional dos antimicrobianos e quimioterápicos trouxe dificuldades, sendo a maior delas representada pela progressiva resistência bacteriana aos fármacos. 1 As bactérias podem ser classificadas em sensíveis e resistentes aos antimicrobianos. Em geral, classificam-se como resistentes, as bactérias que crescem "in vitro", nas concentrações médias que os antimicrobianos atingem no sangue, quando administrados por via oral. São sensíveis as que não crescem nestas concentrações. 2 De acordo com Trabulsi e Toledo 2 , a resistência bacteriana pode ser natural ou adquirida. A natural corresponde a uma característica da espécie bacteriana, e a adquirida, à característica de uma ou mais amostras da espécie. A aquisição de resistência por uma célula bacteriana sensível é sempre decorrente de uma alteração genética.O gênero Listeria apresenta uma susceptibilidade uniforme aos antimicrobianos contra bactérias Gram positivas. 3 Os antimicrobianos mais indicados no tratamento da listeriose são : tetraciclina, coumercina, rifampicina, cloranfenicol, eritromicina e ampicilina, sendo esta última adicionada de um antibiótico aminoglicosídio. 4,5 Marth 6 relata que a maioria das cepas é sensível à tetraciclina, cloranfenicol, eritromicina e cefalotina. Ampicilina, rifampicina, ou penicilina associada com gentamicina são os antimicrobianos de escolha para o tratamento de listeriose. 7 A associação de trimetoprim com uma sulfonamida como a sulfametoxazole no co-trimoxazole, é considerada como uma segunda opção para o tratamento. 8 Para o tratamento da bacteremia e da mulher grávida com listeriose, utiliza-se a vancomicina e a eritromicina, respectivamente. 7 Mesmo com o uso destes fármacos, a terapia com antimicrobian...

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“…Human listeriosis is a public health problem of low incidence but high mortality, requiring prompt diagnosis and adequate antibiotic therapy (1). Over the last 2 decades a high number of food-borne listeriosis outbreaks have occurred, some with high mortality rates (2,13,19).…”

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confidence: 99%

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

Rodas-Suárez

Flores-Pedroche

Betancourt-Rule

et al. 2006

Appl Environ Microbiol

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We analyzed the presence of Listeria spp. in oyster, fish, and seawater samples and tested isolates for antibiotic sensitivity. Listeria monocytogenes was found in 4.5% of fish samples and 8.3% of seawater samples and was not recovered from oysters. Multiresistant environmental strains were found, representing a potential threat to human health.Human listeriosis is a public health problem of low incidence but high mortality, requiring prompt diagnosis and adequate antibiotic therapy (1). Over the last 2 decades a high number of food-borne listeriosis outbreaks have occurred, some with high mortality rates (2,13,19). Antibiotic resistance and inefficient empirical treatment of Listeria infections could be responsible for this increased mortality (4). Since the first multiresistant Listeria monocytogenes strain was observed in France (14), different antibiotic resistance patterns in environmental, food, and clinical sources have been reported (7,12,20). Information on the presence of Listeria monocytogenes in Mexico is scarce, and the frequency of listeriosis is unknown. The purpose of this study was to determine the presence of Listeria spp. in fish, oysters, and saline waters in an area where fish are caught for local and regional consumption and to determine the sensitivities of the L. monocytogenes isolates to different antimicrobial agents.A total of 66 oyster, 66 fish, and 144 estuarine water samples were collected over a 12-month period (June 2001 to May 2002) from 12 sites of the Pueblo Viejo lagoon, Veracruz, Mexico (Fig. 1). Fish and oyster samples were transported on dry ice in separate thermal containers, and estuarine water samples were collected in sterile plastic bottles (Nalgene) and transported to the laboratory on ice. Oyster and fish samples were processed as previously described (9). Seawater samples were filtered through a 14-cm-diameter and 0.45-m-pore membrane (Millipore). Twenty-five milliliters of seawater or 25 g oyster or fish samples was added to 225 ml enrichment broth (EB; Merck) and incubated at 30°C for 24 to 48 h. The filter used for the water samples was washed with 100 ml peptone solution (0.1%), added to 225 ml EB, and incubated at 4°C for 7 days. Afterwards, a 0.1-ml sample was streaked in Oxford agar (Oxoid) and incubated at 30°C for 24 to 48 h. L. monocytogenes isolates were identified and serotyped as previously described (8). Antibiotic sensitivity was assessed using the Kirby-Bauer disk diffusion assay. The test and control strains were seeded in Mueller-Hinton agar supplemented with 0.5% defibrinated sheep blood and 0.1% esculin (17). Commercially available disks (Bio-Rad) with the following antibiotics were used: ampicillin, cephalothin, cefotaxime, ceftazidime, cefuroxime, dicloxacillin, erythromycin, gentamicin, pefloxacin, penicillin, tetracycline, and trimethoprim-sulfamethoxazole. MICs at which 50% of the isolates were inhibited (MIC 50 s) and MIC 90 s were calculated by following the CLSI (formerly NCCLS) guidelines (11). L. monocytogenes ATCC 19114, Escherichia co...

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Susceptibility of Listeria monocytogenes isolated from food in Italy to antibiotics

Cited by 51 publications

References 24 publications

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)

Resistência antimicrobiana de bactérias do gênero Listeria spp: isoladas de carne moída bovina

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

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