Survivin as a diagnostic and therapeutic marker for thyroid cancer

Mahmoudian-Sani, Mohammad-Reza; Alghasi, Arash; Saeedi-Boroujeni, Ali; Jalali, Akram; Jamshidi, Mohammad; Khodadadi, Ali

doi:10.1016/j.prp.2019.01.025

Cited by 16 publications

(11 citation statements)

References 46 publications

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“…Then the level of Bcl-2 family pro-apoptotic protein Bax could significantly be up-regulated after GXI treatment had been detected. Finally, we detected the level of Bcl-2 family anti-apoptotic protein Bcl-2, Bcl-XL, Mcl-1, and Survivin, these proteins were overexpressed in tumor cells and helped tumor cells avoid apoptosis [25,26]. GXI could downregulate the expressions of these proteins and had concentration dependence.…”

Section: Discussionmentioning

confidence: 99%

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

Jin

Shi

Liu

et al. 2019

IJMS

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Xanthones are important chemical constituents of Garcinia xanthochymus and varied bioactivities including cytotoxicity. However, their anti-tumor mechanism has remained unknown. Here, we isolated and identified a new xanthone named garciniaxanthone I (1) and five known compounds from the bark of G. xanthochymus. Their structures were elucidated by NMR analysis and HRESIMS. The anti-proliferation activities of all isolated compounds were evaluated on four human tumor cell lines (HepG2, A549, SGC7901, MCF-7). The results demonstrated that the anti-proliferation activity of xanthone was related to the number and location of prenyl groups. We further found that garciniaxanthone I (GXI) could induce HepG2 apoptosis and enhance the expression of cleaved caspase-8, caspase-9, and caspase-3. GXI could also increase Bax level and concurrently reduce the overexpression of Bcl-2, Bcl-XL, Mcl-1, and surviving in HepG2 cells. Moreover, GXI could inhibit cell migration of HepG2 cells by inhibiting the expressions of MMP-7 and MMP-9. In summary, our study suggests that GXI could induce HepG2 apoptosis via the mitochondrial pathway and might become a lead compound for liver cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

Jin

Shi

Liu

et al. 2019

IJMS

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“…Survivin, an apoptosis inhibitor, plays a crucial role in tumor cell differentiation, progression, and invasion ( Jaiswal et al, 2015 ). In particular, surviving expression has been shown to be an independent prognostic factor based on overexpression in neoplastic tissues and low expression in normal tissues ( Veiga et al, 2019 ; Mahmoudian-Sani et al, 2019 ). The results of our study indicated that HA-DOX-CDDP showed greater therapeutic efficacy against breast cancer than free drugs.…”

Section: Discussionmentioning

confidence: 99%

Development of a Hyaluronic Acid-Based Nanocarrier Incorporating Doxorubicin and Cisplatin as a pH-Sensitive and CD44-Targeted Anti-Breast Cancer Drug Delivery System

et al. 2020

Front. Pharmacol.

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Tumor-targeting nanomaterial-based chemotherapeutic drug delivery systems have been shown to represent an efficacious approach for the treatment of cancer because of their stability in blood circulation and predictable delivery patterns, enhanced tumor-selective drug accumulation, and decreased toxicity to normal tissues. The cell-surface transmembrane glycoprotein CD44 binds to the extracellular domain of hyaluronic acid (HA), and is overexpressed in breast, ovarian, lung, and stomach cancer. In this study, an HA-based nano-carrier incorporating doxorubicin (DOX) and cisplatin (CDDP) was synthesized as a CD44-targeting anti-cancer drug delivery system, and its tumor inhibition effects against CD44 + breast cancer cells were evaluated in vitro and in vivo . These dual drug-loaded HA micelles (HA-DOX-CDDP) exhibited significantly enhanced drug release under acidic conditions, and showed higher cellular uptake and stronger cellular growth inhibition than free drugs against 4T1 (CD44 + ) breast cancer cells. In contrast, no significant differences in growth inhibition and cellular uptake were observed between HA-DOX-CDDP and free drugs in NIH-3T3 (CD44 - ) control cells. Furthermore, HA-DOX-CDDP micelles exhibited stronger inhibitory effects and lower systemic toxicity than free drugs in a 4T1 mammary cancer-bearing mouse model, as determined using immunofluorescence and histological analyses. Therefore, HA-DOX-CDDP micelles represent a promising drug delivery system that exhibits acid-sensitive drug release, CD44-targeted delivery, and excellent biocompatibility and biodegradation. These properties resulted in excellent tumor accumulation and reduced adverse effects, indicating that HA-DOX-CDDP micelles have promising potential applications in chemotherapy for breast cancer.

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“…Survivinalso known as baculoviral inhibitor of apoptosis repeatcontaining 5-is the newest member of a family of proteins that is involved in inhibiting apoptosis; it was recently suggested as a novel biomarker of cancer. 29,30 Overexpression of survivin in tumors is generally associated with poor prognosis and high drug resistance. 31 Our previous study implicated survivin as a critical regulator of tumor cell apoptosis in GBC.…”

Section: Discussionmentioning

confidence: 99%

<p>Long Noncoding RNA <em>NEAT1</em> Upregulates Survivin and Facilitates Gallbladder Cancer Progression by Sponging microRNA-335</p>

Yang¹,

Tang²,

Duan³

et al. 2020

OTT

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Background: Gallbladder cancer (GBC) is the most common cancer of the biliary tract, but molecularly targeted therapies are not available for GBC. Loss of microRNA (miR)-335 expression may be a useful predictor of clinical outcomes and the reversal of its loss of expression may be a useful treatment strategy for GBC. In this study, we investigated whether a long noncoding RNA, nuclear paraspeckle assembly transcript 1 (NEAT1) sponges miR-335 in GBC cells. Materials and Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry were used to determine the expression of miR-335; NEAT1; survivin; and Ki67 in GBC cell lines (GBC-SD and SGC-996) and tissue samples from patients (n = 25). Cell Counting Kit-8, colony-formation, and Transwell migration and invasion assays were performed to measure cell proliferation, migration, and invasion. Bioinformatic analysis and dual-luciferase reporter assays were utilized to analyze correlativity. Results: miR-335 overexpression resulted in inhibition of GBC cell proliferation and invasion. In addition, knockdown of NEAT1 resulted in downregulation of survivin expression. As NEAT1 competitively "sponges" miR-335, NEAT1 knockdown resulted in inhibited GBC cell proliferation and invasion in vitro and GBC tumor growth in vivo. Furthermore, NEAT1 was found to be upregulated in GBC samples, and its expression was inversely correlated with miR-335 levels, but positively correlated with survivin levels. Conclusion: These findings indicate that NEAT1 promotes survivin expression by functioning as a competitive endogenous RNA for miR-335 in GBC cells; thus, we have identified a potential biomarker and target for GBC diagnosis and therapy.

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Survivin as a diagnostic and therapeutic marker for thyroid cancer

Cited by 16 publications

References 46 publications

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

Development of a Hyaluronic Acid-Based Nanocarrier Incorporating Doxorubicin and Cisplatin as a pH-Sensitive and CD44-Targeted Anti-Breast Cancer Drug Delivery System

<p>Long Noncoding RNA <em>NEAT1</em> Upregulates Survivin and Facilitates Gallbladder Cancer Progression by Sponging microRNA-335</p>

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