Survival, Virulent Characteristics, and Transcriptomic Analyses of the Pathogenic Vibrio anguillarum Under Starvation Stress

Gao, Xiaojian; Pi, Daming; Chen, Nan; Li, Xixi; Liu, Xiaodan; Yang, Hui; Wei, Wanhong; Zhang, Xiaojun

doi:10.3389/fcimb.2018.00389

Cited by 22 publications

(16 citation statements)

References 54 publications

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“…Concisely, Vibrio anguillarum isolated from A. aurita Baltic Sea polyps is widely distributed in marine and estuarine environments around the world ( 92 – 94 ). It is a global causative agent of vibriosis in marine fish and shellfish species, because it can efficiently grow and proliferate under environmental stress ( 95 ). Pseudoalteromonas espejiana isolated from artificial seawater is a ubiquitous bacterium abundantly found in marine environments and often associated with marine multicellular organisms ( 96 ).…”

Section: Methodsmentioning

confidence: 99%

The Native Microbiome is Crucial for Offspring Generation and Fitness of Aurelia aurita

Weiland‐Bräuer

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Langfeldt

et al. 2020

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All multicellular organisms are associated with microbial communities, ultimately forming a metaorganism. Several studies conducted on well-established model organisms point to immunological, metabolic, and behavioral benefits of the associated microbiota for the host. Consequently, a microbiome can influence the physiology of a host; moreover, microbial community shifts can affect host health and fitness. The present study aimed to evaluate the significance and functional role of the native microbiota for life cycle transitions and fitness of the cnidarian moon jellyfish Aurelia aurita. A comprehensive host fitness experiment was conducted studying the polyp life stage and integrating 12 combinations of treatments with microbiota modification (sterile conditions, foreign food bacteria, and potential pathogens). Asexual reproduction, e.g., generation of daughter polyps, and the formation and release of ephyrae were highly affected in the absence of the native microbiota, ultimately resulting in a halt of strobilation and ephyra release. Assessment of further fitness traits showed that health, growth, and feeding rate were decreased in the absence and upon community changes of the native microbiota, e.g., when challenged with selected bacteria. Moreover, changes in microbial community patterns were detected by 16S rRNA amplicon sequencing during the course of the experiment. This demonstrated that six operational taxonomic units (OTUs) significantly correlated and explained up to 97% of fitness data variability, strongly supporting the association of impaired fitness with the absence/presence of specific bacteria. Conclusively, our study provides new insights into the importance and function of the microbiome for asexual reproduction, health, and fitness of the basal metazoan A. aurita. IMPORTANCE All multicellular organisms are associated with a diverse and specific community of microorganisms; consequently, the microbiome is of fundamental importance for health and fitness of the multicellular host. However, studies on microbiome contribution to host fitness are in their infancy, in particular, for less well-established hosts such as the moon jellyfish Aurelia aurita. Here, we studied the impact of the native microbiome on the asexual reproduction and on further fitness traits (health, growth, and feeding) of the basal metazoan due to induced changes in its microbiome. We observed significant impact on all fitness traits analyzed, in particular, in the absence of the protective microbial shield and when challenged with marine potentially pathogenic bacterial isolates. Notable is the identified crucial importance of the native microbiome for the generation of offspring, consequently affecting life cycle decisions. Thus, we conclude that the microbiome is essential for the maintenance of a healthy metaorganism.

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Section: Methodsmentioning

confidence: 99%

The Native Microbiome is Crucial for Offspring Generation and Fitness of Aurelia aurita

Weiland‐Bräuer

Pinnow

Langfeldt

et al. 2020

mBio

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“…The PCR confirmed the presence of empA, vah1, vah3, vah4, flaA, angM, ang Rgenes in all tested isolates. Using the same primers, Gao, Pi [17] amplified empA, vah1, vah2, vah3, vah4, vah5, rtxA, flaA, but not angM, angR genes from stressed starved V. anguillarum isolate for six months.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Pathogenic Vibrio anguillarumAmong Oreochromis niloticusFish Fingerlings Infected with Saprolegniasis Around Qarun Lake

et al. 2021

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V IBRIO anguillarum causes severe economic losses in fish and shellfish species and poses public health concerns. This study aimed to provide some information about the prevalence and virulence determinants of V. anguillarum isolated from the farmed Oreochromisniloticus fish fingerlings nearby Qarun Lake, El-Fayoum Governorate, Egypt. PCRtargeting the rpoA (RNA polymerase alpha subunit)gene was done forconfirmation of Vibriospp. Moreover, V. anguillarum isolates were biochemically identified and confirmed by 16S rRNA gene sequence.V. anguillarum isolates produced caseinase, gelatinase, lipase, lecithinase, haemolysins, and possessed biofilm formation. The virulence-related genes;empA, vah1, vah3 and vah4, flaA,angM, and angRwere detected in all tested isolates. Therefore, proper identification of V.anguillarumis crucial to better understand the ecology and distribution patterns of such pathogen within Egyptian aquaculture. Moreover, the water quality in these farms near Qarun Lake was less supportive for larval rearing activities of O. niloticus fish.

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“…Each group of the M. nipponensis was maintained in a 50‐L aquarium (30 shrimp per aquarium) at 25°C water temperature, and the farming water was aerated with 5.0 mg/L of dissolved oxygen. The isolate NJ1 was grown overnight at 100 ml of LB liquid medium at 28℃ with shaking at 200 rpm, enumerated by a 10‐fold serial dilution and spread plate methods (Gao et al., 2018). Five groups were challenged by immersion infection and retained in 30 L of water containing the bacteria at a final concentration of 2.1 × 10 8 , 2.1 × 10 7 , 2.1 × 10 6 , 2.1 × 10 5 and 2.1 × 10 4 CFU/ml as test group respectively.…”

Section: Methodsmentioning

confidence: 99%

Aeromonas veronii associated with red gill disease and its induced immune response in Macrobrachium nipponense

et al. 2020

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Aeromonas veronii is a freshwater bacterium associated with many diseases in aquatic animals. In this study, the pathogenic of A. veronii was isolated from diseased Macrobrachium nipponense showing signs of weakness, poor appetite and red gills in some farms in Jiangsu province, China. The pathogenicity of A. veronii to M. nipponense was determined by challenge experiments. Results of artificial challenge showed A. veronii NJ1 was pathogenic to M. nipponense, and the LD50 was 4.5 × 106 CFU/ml at 7 d post infection. Detection virulence factors of the A. veronii NJ1 showed that A. veronii NJ1 produced caseinase, lipase, amylase, lecithinase, gelatinase and haemolysin. Detection of virulence‐related genes showed that A. veronii NJ1 was positive for aerolysin (aer), outer membrane protein (ompA), adhesin (aha), flagellin (flaA, flgH, flgM, flgN), lipoidase (lip), cytotonic enterotoxins (act, alt, ast), serine protease (ser), DNases (exu), haemolysin (hlyA), type IV pilus (tapA) and fimbrillin (fim). Antibiotic sensitivity testing indicated A. veronii NJ1 was susceptible to quinolones, but resistant to penicillins. Additionally, the immune‐related gene expression in M. nipponense was evaluated, and the results showed that anti‐lipopolysaccharide factor (ALF1, ALF2, ALF3), Cusrin1, Cusrin2, Cusrin3, Haemocyanin, i‐lysozyme and Prophenoloxidase were significantly upregulated from 6 to 24 hr after A. veronii infection. The results of our study suggested that A. veronii was an aetiological element in the mass mortalities of M. nipponense and this study provided preliminary insights into the diversity in the immune response of M. nipponense to the A. veronii infection.

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Survival, Virulent Characteristics, and Transcriptomic Analyses of the Pathogenic Vibrio anguillarum Under Starvation Stress

Cited by 22 publications

References 54 publications

The Native Microbiome is Crucial for Offspring Generation and Fitness of Aurelia aurita

The Native Microbiome is Crucial for Offspring Generation and Fitness of Aurelia aurita

Prevalence of Pathogenic Vibrio anguillarumAmong Oreochromis niloticusFish Fingerlings Infected with Saprolegniasis Around Qarun Lake

Aeromonas veronii associated with red gill disease and its induced immune response in Macrobrachium nipponense

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