Survival Rate of Microbes after Freeze-Drying and Long-Term Storage

Miyamoto‐Shinohara, Yukie; Imaizumi, Tsutomu; Sukenobe, Junji; Murakami, Yukie; Kawamura, Sugio; Komatsu, Yasuhiko

doi:10.1006/cryo.2000.2282

Cited by 127 publications

(67 citation statements)

References 8 publications

(8 reference statements)

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“…It was demonstrated that Lactococcuslactis is quiter esistant to freezing,t hawing andd ehydration during thep rocesses of freezing and freeze-drying, which is useful from ac ommercial viewpoint. This result was expected because many bacteria are knownt os urvive lyophilisation well [1].…”

Section: Discussionmentioning

confidence: 76%

“…Adequate shelf life, high cell viability and activity are ther equirements for bacterial formulations.L actic acid bacteria and other biological materials used as starter cultures in the dairyi ndustry or as silage preservantsa re oftenp reserved by freezing and/or freezedrying resulting in products with excellentl ong-terms tabilityi nm ost cases [ 1].…”

Section: Introductionmentioning

confidence: 99%

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Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Berner

2006

Sci Pharm

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The effecto fd ifferentp rotectants and the impact of the initial cell densityo n the viability of Lactococcus lactis Sr. 3.54 subjected to freeze-thawing and freezedrying was studied. Several additivesw ere tested as protective agents against freezing and drying injuries. Maximumv iability of the cells after freeze-thawing was obtained with sucrose and skim milk mixtures as protective agents (78% viability).

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Berner

2006

Sci Pharm

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“…By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al, 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al, 2000.To increase the survival rates of S. cerevisiae strains, we tested the applicability of L-drying, in which cells are desiccated by vacuum evaporation at room temperature. In contrast, during freeze-drying, cells are desiccated by sublimation.…”

mentioning

confidence: 99%

Survival of yeasts stored after freeze-drying or liquid-drying

Miyamoto‐Shinohara¹,

Nozawa²,

Sukenobe³

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionFreeze-drying and L-drying are popular methods of preserving microorganisms, because long-term viability is excellent in most cases and the storage and distribution requirements are simple. By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al., 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al., 2000.To increase the survival rates of S. cerevisiae strains, we tested the applicability of L-drying, in which cells are desiccated by vacuum evaporation at room temperature. In contrast, during freeze-drying, cells are desiccated by sublimation. L-drying is effective for the long-term preservation of cultures sensitive to freezedrying (Malik, 1990(Malik, , 1999. In this research, yeast strains were L-dried according to the method of the Institute for Fermentation, Osaka (Banno et al., 1979), in which the cell suspension was dispensed into a glass ampoule (in the same way as for freeze-drying) and a cotton wool plug was inserted to prevent the temperature in the ampoule from falling to the freezing point (Iijima and Sakane, 1973).To clarify the mechanisms underlying tolerance to L-drying and freeze-drying, we investigated the survival of type strains of various yeast species. The survival J. Gen. Appl. Microbiol., 56, 107 119 (2010) We investigated

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“…Many microorganisms can survive in a vacuum for many years. Not only spore forms but also vegetative cells can be stored alive in a vacuum (Miyamoto-Shinohara et al, 2000).…”

Section: What Is Needed For Lifementioning

confidence: 99%