Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Berner, Diana

doi:10.3797/scipharm.2006.74.137

Cited by 56 publications

(31 citation statements)

References 13 publications

(15 reference statements)

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“…The bacterial counts reached detection limits in 30 and 45 day storage respectively. Similar results have been noted by Berner and Viernstein (2006) who reported water and saline to be poor cryoprotectants.…”

Section: Freeze Drying and Storage Viability Of Immobilized Lactic Acsupporting

confidence: 90%

Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria

Jagannath

Raju

Bawa

2010

LWT - Food Science and Technology

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Section: Freeze Drying and Storage Viability Of Immobilized Lactic Acsupporting

confidence: 90%

Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria

Jagannath

Raju

Bawa

2010

LWT - Food Science and Technology

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“…Low temperature (freezing or refrigeration) and freeze‐drying (lyophilization) are the most common techniques used to stabilize probiotics. However, lyophilized cultures are more adequate than frozen ones based on both transport and storage costs (Berner and Viernstein ).…”

Section: Introductionmentioning

confidence: 99%

“…Many factors have been reported to affect the freeze‐drying survival of LAB and their subsequent storage such as initial cell concentration, growth media, freezing rate and temperature and storage temperatures (Carvalho et al . ; Berner and Viernstein ; Schoug et al . ; Schoug Bergenholtz et al .…”

Section: Introductionmentioning

confidence: 99%

Survival and beneficial properties of lactic acid bacteria from raniculture subjected to freeze-drying and storage

et al. 2013

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Aim: To evaluate the effect of freeze-drying and storage conditions on the viability and beneficial properties of lactic acid bacteria (LAB) for raniculture. Methods and Results: Lactococcus lactis CRL 1584, L. lactis CRL 1827, Lactococcus garvieae CRL 1828 and Lactobacillus plantarum CRL 1606 viability under different conditions was studied. 10% lactose and 5% skim milk + 5% lactose were excellent lyoprotectants, but 5% skim milk + 5% lactose and whey protein concentrated (WPC) or WPC + sugars were the lower cost lyoprotective options. The effect of temperature depended on both lyoprotectants and storage time. Thus, for Lactococcus, skim milk, skim milk + sucrose and WPC + sucrose were selected for lyophilization and storage at 4°C and skim milk + lactose for 25°C. For Lact. plantarum CRL 1606, the best lyoprotectants for lyophilization and storage at 4°C were milk + sugars and WPS + sucrose and, at 25°C, skim milk + sucrose. Conclusions: Lactic acid bacteria viability after freeze-drying was strainspecific and depended on the lyoprotectant used. Highest viability was obtained when stored at 4°C, and the beneficial properties remained stable for 18 months independently of storage temperature. Significance and Impact of the Study: The studies reported for the first time in this work are of primary interest to obtain dried bacteria to be included in beneficial products for raniculture.

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“…The suspension was frozen at −70°C and lyophilized (Rificor Mod. L‐A‐B4, Argentina) (Berner and Viernstein, ; Kŏs et al., ; Ming et al., ). The viable cell number was monitored periodically by plate counting on MRS agar under reduced oxygen tension.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Lactobacillus paracasei subsp. tolerans isolated from Jenyn's sprat (Ramnogaster arcuata ) as probiotic for juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792)

et al. 2014

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Summary A lactic acid bacterial strain, Lactobacillus paracasei subsp. tolerans F2, isolated from the intestine of Ramnogaster arcuata, was evaluated as a growth promoter in juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1972) farming. In addition, the safety of the strain was assessed according to the FAO recommendations. Strain F2 was susceptible to most antibiotics tested and no evidence of hemolytic activity was found. When the strain of Lactobacillus paracasei subsp. tolerans F2 was administered with food, no adverse effects on health were observed and fish biomass increased 12% more in the treatment group than in the control group. Significant differences were detected in the specific growth rate and feed conversion ratio. In the group receiving Lactobacillus paracasei subsp. tolerans F2‐supplemented feed, quantitative differences in the microbial composition of fish feces with respect to the control group were observed. An important decrease in fungi and enterobacteria was observed in feces from the treatment group, coincident with an increase in lactic acid bacteria. This result would indicate a change in the composition of the intestinal microbiota of fish treated with the putative probiotic. These results suggest that the strain of Lactobacillus paracasei subsp. tolerans F2 has the application potential to improve the performance in rainbow trout farming.

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Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Cited by 56 publications

References 13 publications

Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria

Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria

Survival and beneficial properties of lactic acid bacteria from raniculture subjected to freeze-drying and storage

Evaluation of Lactobacillus paracasei subsp. tolerans isolated from Jenyn's sprat (Ramnogaster arcuata ) as probiotic for juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792)

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