Survival of sea urchin spermatozoa and embryos at very low temperatures

Asahina, Éizo; Takahashi, T.

doi:10.1016/0011-2240(77)90087-6

Cited by 13 publications

(5 citation statements)

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“…It is therefore remarkable that, despite some of the pioneering work in cryopreservation that was done with marine invertebrates such as oysters [68] and sea urchins [31] there has been little research carried out in sea urchin cryopreservation and biobanking thereafter. Only in recent years have researchers re-discovered the usefulness of its cryopreservation and increased their interest in biobanking for different purposes [10,21,[37][38][39][40]43,61,64].…”

Section: Discussionmentioning

confidence: 99%

“…There is general an agreement that membranes are often damaged during the freezing process [56,73,74], and sea urchins are probably not an exception. Although there is research published on sea urchin embryos reporting positive results after freezing, such as an initial 90% survival reported with H. pulchehimus, S. intermedius and S. nudus embryos, the resulting larvae suffered severe abnormalities, and development was impaired after only three days [31,32,59,75]. Cryopreserved embryos were able to be reared to juveniles for S. intermedius [33,34,61] and, despite suffering some abnormalities, 0.1%-0.2% survived to settlement.…”

Section: Cryopreservation and Cryoinjuriesmentioning

confidence: 99%

“…Gametes and embryo cryopreservation protocols and cryopreservation effects have been studied and published for 11 sea urchin species, the most common of which are Hemicentrotus pulcherrimus, Strongylocentrotus nudus and Stongylocentrotus intermedius by Asahina and Takahashi [31,32], Naidenko et al [33] and Naidenko and Koltsova [34], , Tetrapigus niger by Barros et al [35,36], Evechinus chloroticus by Adams and Hessian [37] and Adams et al [38] and Paracentrotus lividus embryos by Bellas and Paredes [39] and Paredes and Bellas [10].…”

Section: Introductionmentioning

confidence: 99%

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Biobanking of a Marine Invertebrate Model Organism: The Sea Urchin

Paredes

2016

JMSE

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The sea urchin has long been used as an invertebrate model organism in developmental biology, membrane transport and sperm oocyte interactions, and for the assessment of marine pollution. This review explores the effects of cryopreservation and biobanking in the biology and development of sea urchins, all the way from germaplasm through to juveniles. This review will provide an integral view of the process and all that is known so far about the biology of cryopreserved sea urchins, as well as provide an insight on the applications of the biobanking of these model organisms.

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Section: Discussionmentioning

confidence: 99%

Section: Cryopreservation and Cryoinjuriesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biobanking of a Marine Invertebrate Model Organism: The Sea Urchin

Paredes

2016

JMSE

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“…The following supporting information can be downloaded at: , Figure S1: Cooling rate calculation; Table S1: List of sea urchins catalogued in Galician waters; Table S2: Updated list of cryopreservation studies published on Sea urchins [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ]; Table S3: Fertilization Data Temperature storage experiment.…”

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confidence: 99%

Handling, Reproducing and Cryopreserving Five European Sea Urchins (Echinodermata, Klein, 1778) for Biodiversity Conservation Purposes

Paredes

Campos

Lago

et al. 2022

Animals

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In this work, five local sea urchin species found in European waters were studied. Four were regular species: Sphaerechinus granularis, Psammechinus miliaris, Echinus esculentus (Linnaeus, 1758) and the edible sea urchin Paracentrotus lividus; and one was an irregular species, Echinocardium cordatum. These five species of sea urchins have been studied regarding their fertility, toxicity of cryoprotecting agents, cryopreservation of different cell types and chilling injury. The baseline fertility is similar in P. lividus, P. miliaris and S. granularis. Nonetheless, the sperm:egg ratio, contact time and development of the fertilization envelope would need to be studied further on a case-by-case basis. Sperm can be maintained inactively in the gonad (4 °C), and oocytes also maintain quality in sea water (4 °C), even after 72 h. Sperm was cryopreserved for four species with some post-thaw intra specific variability, and embryo cryopreservation was only possible for S. granularis. Overall, this study provided a wider vision of the biology and reproduction of these species that will help us develop tools for their biodiversity conservation through cryopreservation.

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“…a cooling rate of 5.5°C/min; only 11-12% of blastulae were capable of normal development [7]. A lower cooling rate (0.5-3°C/min and below) was ineffective and inevitably resulted in cell lysis among thawed zygotes [11,24], but this cooling rate was acceptable to preserve sea urchin embryos at the blastula stage [11,47,48].…”

Section: Introductionmentioning

confidence: 99%