Survival of Ralstonia solanacearum in infected tissues of Capsicum annuum and in soils of the state of Pernambuco, Brazil

Felix, Kátia Cilene S.; Souza, Elineide Barbosa de; Michereff, Sami Jorge; Mariano, R. L. R.

doi:10.1007/s12600-011-0200-6

Cited by 21 publications

(14 citation statements)

References 25 publications

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“…As plantas com 30 dias da semeadura foram inoculadas pelo método do corte de raízes, fazendo-se com auxílio de um bisturi um corte semicircular no substrato perto do caule da planta, no qual foram depositados 15 ml da suspensão bacteriana (5x10 8 UFC ml -1 ) (FELIX et al, 2012).…”

Section: Methodsunclassified

Parâmetros genéticos de famílias F2:3 de tomateiro para resistência à Ralstonia pseudosolanacearum e Ralstonia solanacearum

et al. 2018

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Resumo: O tomateiro (Solanum lycopersicum) é uma cultura de grande importância para o Brasil, no entanto se mostra suscetível a várias bacterioses. Objetivou-se estudar os parâmetros genéticos de 43 familias F 2:3 de tomateiro na resistência à R. pseudosolanacearum e R. solanacearum. As famílias foram obtidas a partir do cruzamento entre as cultivares Yoshimatsu (resistente) e IPA-7 (susceptível). Foram conduzidos dois experimentos em casa de vegetação da Universidade Federal Rural de Pernambuco, sendo um para cada espécie. Utilizou-se o delineamento em blocos casualizados com quatro repetições e 45 tratamentos. Avaliou-se a incidência e severidade da murcha bacteriana, por meio de escala discritiva de notas, aos 10 e 20 dias após a inoculação. Observou-se menor influência do ambiente, e maior variabilidade para R. pseudosolanacearum do que para R. solanacearum, tanto entre quanto dentro de famílias. Para R. pseudosolanacearum recomenda-se priorizar a selecção com 20 dias entre e dentro de famílias; para R. solanacearum deve-se selecionar apenas aos 20 dias, entre familias. Foram identificadas maiores quantidades de plantas resistentes para R. pseudosolanacearum em qualquer época de avaliação do que para R. solanacearum. Palavras-chave: Severidade; Murcha bacteriana; Solanum LycopersicumAbstract: The tomato (Solanum lycopersicum) is an important crop in Brazil, however, it is susceptible to different types of bacteria races. The objective was to study the genetic parameters of 43 F 2:3 families of tomato in resistance to R. pseudosolanacearum and R. solanacearum. The families were obtained from the cross between the cultivars Yoshimatsu (resistant) and IPA-7 (susceptible). Two experiments were carried out in a greenhouse at Universidade Federal Rural de Pernambuco, one for each species of the genus R. spp. A randomized block design was used with four replications and 45 treatments. There is less influence of the environment, and greater variability for R. pseudosolanacearum than for R. solanacearum, both among and within families. For R. pseudosolanacearum it is recommended to select 10 days between families, and 20 days between and within families. R. solanacearum should be selected from families only at 20 days. Larger amounts of resistant plants were identified for R. pseudosolanacearum at any time of evaluation than for R. solanacearum.

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Section: Methodsunclassified

Parâmetros genéticos de famílias F2:3 de tomateiro para resistência à Ralstonia pseudosolanacearum e Ralstonia solanacearum

et al. 2018

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“…Os ácidos foram aplicados por imersão de raízes e pulverização, conforme já descrito. Três dias após a aplicação dos produtos, as plantas foram inoculadas individualmente fazendo-se, com auxílio de um bisturi inserido no solo, um corte em formato de meia lua no sistema radicular, no qual foram depositados 15 mL da suspensão bacteriana contendo 5 x 10 8 UFC mL -1 (Félix et al, 2012). Como testemunhas, foram utilizadas plantas tratadas com água destilada pelos dois métodos.…”

Section: Methodsunclassified

Ácidos orgânicos no controle da murcha bacteriana do pimentão

García

Souza

Mariano

2014

Agraria

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RESUMONeste estudo foi analisado o efeito de ácidos orgânicos de ação sanitizante no controle da murcha bacteriana do pimentão (Capsicum annuum L.) ocasionada por Ralstonia solanacearum (Smith) Yabuuchi et al. Foram avaliadas, in vitro, a sensibilidade da bactéria aos ácidos ascórbico, cítrico e láctico (0,25, 0,5, 1, 2,5, 5 e 10%) e a mistura estável dos ácidos ascórbico 1%, cítrico 0,475% e láctico 0,475% (0, 0,8, 2,5, 5, 10 e 100%) (v:v). Após teste de fitotoxidez, a mistura estável 0,8% e ácidos ascórbico, cítrico e láctico 1% foram selecionados e testados para redução da severidade da murcha sendo aplicados por imersão de raízes ou pulverização da parte aérea. O patógeno foi inoculado por ferimento de raízes e deposição do inóculo. In vitro, R. solanacearum mostrou alta sensibilidade ao ácido láctico 10% e média aos ácidos láctico 5 e 2,5%, ascórbico e cítrico 10%, além da mistura estável 100%. Todos os tratamentos aplicados por imersão de raízes elevaram significativamente o período de incubação (PI) e reduziram a área abaixo da curva de progresso da doença (AACPD), destacando-se o ácido láctico 1% (100%). Os ácidos cítrico e láctico 1% e a mistura estável 0,8%, também elevaram o PI e reduziram a AACPD em até 46,1% quando aplicados por pulverização. Palavras-chave: ácido ascórbico, ácido cítrico, ácido láctico, Capsicum annuum, Ralstonia solanacearum Organic acids on the control of bacterial wilt of bell pepper ABSTRACTThe effect of disinfectant organic acids on the control of bacterial wilt of sweet pepper (Capsicum annuum L.) caused by Ralstonia solanacearum (Smith) Yabuuchi et al. was evaluated. The in vitro sensitivity of bacteria to the ascorbic, citric and lactic (0.25, 0.5, 1, 2.5, 5 and 10%) acids and the stable mixture of the ascorbic (1%), citric (0.475%) and lactic (0.475%) acids (0.4, 0.8, 2.5, 5, 10 and 100%) (v/v) was evaluated. After the phytotoxicity test the 0.8% stable mixture and ascorbic, citric and lactic acids 1% were selected and tested for bacterial wilt severity reduction, by root immersion or shoot spraying. The pathogen was inoculated by wounding roots and deposition of the inoculum. In vitro, R. solanacearum showed high sensitivity to lactic acid 10% and medium sensitivity to lactic acid (5 and 2.5%), ascorbic and citric acids 10% and stable mixture 100%. All treatments applied by root immersion significantly increased the incubation period (IP) and reduced the area under the disease progress curve (AUDPC), mainly lactic acid 1% (100%). The citric and lactic acids 1% and stable mixture 0.8% also increased IP and reduced AUDPC until 46.1% when applied by shoot spraying.

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“…Entry via true seed is also possible but risks for seed infection and transmission from seed to seedling are limited (CABI, 2018). The pathogen can survive in soil, either in association with plant debris or as a free-living saprophyte (Felix et al, 2012). Given the overlap in the host range for the different phylotypes/species within the species complex (Table 2), there is a general overlap in the pathways of entry for the different phylotypes/species.…”

Section: Entrymentioning

confidence: 99%

Pest categorisation of the Ralstonia solanacearum species complex

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2019

EFS2

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Following a request from the European Commission, the EFSA Panel on Plant Health performed a pest categorisation of the Ralstonia solanacearum species complex (RSSC), a distinguishable cosmopolitan group of bacterial plant pathogens (including R. solanacearum, Ralstonia pseudosolanacearum and two subspecies of Ralstonia syzygii) of the family Burkholderiaceae. The RSSC causes bacterial wilt in solanaceous crops, such as potato, tomato and pepper, but can also cause wilts in other important food crops such as fruit banana, plantain banana and cassava. The pest survives in the soil, and a number of weed species can also be infected by the pest, often asymptomatically. The RSSC is regulated in Council Directive 2000/29/EC (Annex IAII) (indicated by its former name R. solanacearum, as delimited by Yabuuchi et al.) as a harmful organism whose introduction into the EU is banned. In addition, Council Directive 1998/57/EC (amended by Commission Directive 2006/63/CE) concerns the measures to be taken within EU Member States (MS) against the RSSC to (a) detect it and determine its distribution, (b) prevent its occurrence and spread, and (c) control it with the aim of eradication. The pest is present in several EU MS, but in all cases with a restricted distribution and under official control. New phylotypes of the RSSC could enter the EU primarily via host plants for planting (including seed tubers). The pest could establish in the EU, as climatic conditions are favourable, hosts are common and the pathogen has high adaptability. Spread is mainly via plants for planting. Substantial crop losses in the EU would occur in the presence of RSSC epidemics. The RSSC is regarded as one of the world's most important phytopathogenic bacteria due to its broad geographical distribution, large host range, aggressiveness, genetic diversity and long persistence in soil and water. The list of hosts and commodities for which the pest is regulated is incomplete due to the high diversity of hosts and the lack of knowledge of the complete host range. Moreover, the comparative epidemiology of the different pathogen species has not yet been studied. The criteria assessed by the Panel for consideration of the RSSC as potential quarantine pest are met, while, for regulated non-quarantine pests, the criterion on the widespread presence in the EU is not met.

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Survival of Ralstonia solanacearum in infected tissues of Capsicum annuum and in soils of the state of Pernambuco, Brazil

Cited by 21 publications

References 25 publications

Parâmetros genéticos de famílias F2:3 de tomateiro para resistência à Ralstonia pseudosolanacearum e Ralstonia solanacearum

Parâmetros genéticos de famílias F2:3 de tomateiro para resistência à Ralstonia pseudosolanacearum e Ralstonia solanacearum

Ácidos orgânicos no controle da murcha bacteriana do pimentão

Pest categorisation of the Ralstonia solanacearum species complex

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