Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

Nugraha, Dendi Krisna; Nishida, Takashi; Tamaki, Yuki; Hiramatsu, Yukihiro; Yamaguchi, Hiroyuki; Horiguchi, Yasuhiko

doi:10.1128/spectrum.00487-23

Cited by 2 publications

(2 citation statements)

References 50 publications

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“…Our investigation did not yield conclusive proof of replication in the contractile vacuole, as demonstrated in V. cholerae ( 23 ) and Bordetella bronchiseptica ( 29 ). Future experiments conducted in low osmotic media may provide insight into whether such conditions promote the invasion of contractile vacuoles, which were not well developed in the MMsalts-MOPS-Glc medium used herein.…”

Section: Discussioncontrasting

confidence: 77%

“…These amoeba-resistant microbes deploy a plethora of vesicular trafficking manipulation strategies to prevent fusion of pathogen-containing vacuoles (PCVs) with lysosomes. Membrane trafficking subversion strategies are multifactorial, species specific, and unknown for most bacterial species hosted by Acanthamoeba PCVs ( 18 , 20 , 27 – 29 )⁠.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii

Rivera,

Valerdi-Negreros,

Vázquez-Enciso

et al. 2024

Microbiol Spectr

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Diverse Acanthamoeba lineages (genotypes) are of increasing clinical concern, mainly causing amoebic keratitis and granulomatous amebic encephalitis among other infections. S. maltophilia ranks among the top 10 most prevalent multidrug-resistant opportunistic nosocomial pathogens and is a recurrent member of the microbiome hosted by Acanthamoeba and other free-living amoebae. However, little is known about the molecular strategies deployed by Stenotrophomonas for an intracellular lifestyle in amoebae and other professional phagocytes such as macrophages, which allow the bacterium to evade the immune system and the action of antibiotics. Our plasmids and easy-to-use microtiter plate co-culture assays should facilitate investigations into the cellular microbiology of Acanthamoeba interactions with Stenotrophomonas and other opportunistic pathogens, which may ultimately lead to the discovery of new molecular targets and antimicrobial therapies to combat difficult-to-treat infections caused by these ubiquitous microbes.

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Section: Discussioncontrasting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii

Rivera,

Valerdi-Negreros,

Vázquez-Enciso

et al. 2024

Microbiol Spectr

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RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

Ma,

Nugraha,

Hiramatsu

et al. 2023

Microbiology and Immunology

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The Gram‐negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high‐throughput transposon sequencing (Tn‐seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN‐deficient mutant strain was defective in tracheal colonization when co‐inoculated with the wild‐type strain. rpoN encodes σ54, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.

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Survival of Bordetella bronchiseptica in Acanthamoeba castellanii

Cited by 2 publications

References 50 publications

Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii

Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

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