Survival and Differentiation of Cultured Retinal Progenitors Transplanted in the Subretinal Space of the Rat

Polyelectrolyte Multilayer Films as Substrates for Photoreceptor Cells

“…[11][12][13][14][15] Retinal transplantation aims to prevent blindness or to restore eyesight either by rescuing photoreceptor cells or by replacing the damaged photoreceptor cells as dissociated cells 11,16,17 or intact sheets. 12,18 Apart from transplantation of cells or tissues from different donor sources (allogeneic, autologous or xenogeneic), tissue engineering approaches are receiving considerable interest to restore impaired eye functions.…”

Bone Marrow‐Derived Stem Cells Can Differentiate into Retinal Cells in Injured Rat Retina

“…On the other hand, neural stem cells can generate hematopoietic cells in vivo, indicating that neural precursor cells are not restricted to the differentiation of neural-lineage cells [15]. There are many studies of retinal transplantation using the newborn or the embryonic Stem Cells ® Original Article brain [16,17] and retina-derived neural cells [18][19][20][21]. Moreover, hippocampus-derived neural stem cells have been shown to differentiate into neural-forming cells, which are then incorporated into the injured retina [22][23][24][25].…”

A rat model for studying neural stem cell transplantation

“…E-mail yuanhp@yahoo.com vivo. By contrast, some reports show that transplanted NSCs have the advantage to not only differentiate into the host cells, but to also migrate widely and automatically trace the focus of infection [6][7][8] . Unfortunately, NSC engineering and transplantation remain a difficult strategy because of the low rate of success.…”

“…The rats were labeled with numbers and were selected at random on a computer into four groups. Under transpupillary observation with the aid of a 90D preset lens (Ocular Instruments, USA) in the surgeon's left hand, and using a binocular ophthalmic surgical microscope (Zeiss, Germany), a 10 μL cell suspension was injected at the equator through the pars plana of the sclera and choroid backwards into the subretinal space of the eye with a 1 mL syringe and a 29-gauge needle, as described previously [8,9] with a few modifi cations. The operations were done carefully to avoid damaging the lens, retina, iris, and vessels.…”