Survival and biochemical characteristics of stored red cells preserved with citrate‐phosphate‐dextrose‐adenine‐one and two and prepared from whole blood maintained at 20 to 24 degrees C for eight hours following phlebotomy

Our previous studies have shown that the use of half-strength citrate anticoagulant improves plasma factor VIII stability while maintaining the viability of red cells and platelets. This study extends these observations to show that the stability of factor VIII is maintained in blood stored at room temperature or 4°C overnight and that in vitro tests indicate comparable quality of red cells and platelets prepared from half-strength citrate donations processed immediately or after overnight storage at room temperature. The exception to this was a more rapid loss of red cell 2,3-diphosphoglycerate, as has previously been observed for full-strength citrate donations.

Section: Red Cell Preparationssupporting

confidence: 57%

Section: Red Cell Preparationsmentioning

confidence: 99%

Studies on the Procurement of Blood Coagulation Factor VIII

Griffin¹,

Bell²,

Prowse³

1988

“…These recovery and survival studies have been initiated. It has also been shown that red cell and platelet preparations from standard CPD.Al dona tions may be prepared 8-15 h after donation [43][44][45]. It will be of interest to determine if this is also possible for half-strength citrate donations and if so whether the improved stability of Vili : C already demonstrated for blood (table II) and plasma ( fig.…”

Section: Platelet Concentratesmentioning

confidence: 98%

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half-Strength Citrate Anticoagulant

Prowse¹,

Waterston²,

Dawes³

et al. 1987

The effect of replacing a standard citrate anticoagulant with one containing half the amount of citrate on the in vitro properties of components prepared from blood donations was investigated. This resulted in a significant improvement in factor VIII stability such that there was little loss during overnight storage, and this was reflected in the factor VIII yield in cryoprecipitate. The quality of cellular components in red cell units stored up to 35 days or platelet concentrates stored up to 7 days was not adversely affected. Although initial levels were similar to those in standard anticoagulant, the extent of fibrinopeptide A generation and complement C3 breakdown in red cell units stored for 35 days in half-strength citrate was somewhat increased.

“…This probably reflected the increase in ATP levels which occurred during the holding of whole blood for 8 h at ambient temperature. Such an increase in ATP levels dur ing the holding of units of whole blood has been observed in a number of instances [5][6][7], Red cell 2,3-DPG levels were also slightly better main tained at the lower storage temperature. This was observed when AS-1 red blood cells were stored for 7 days sub sequent to either a 1-or an 8-hour holding time for units of whole blood (table 1).…”

Section: Preparation and Storage Of Red Blood Cellsmentioning

confidence: 59%

Storage of ADSOL-Preserved Red Cells at 2.5 and 5.5°C: Comparable Retention of in vitro Properties(1)

Moroff¹,

Holme²,

Keegan³

et al. 1990

Previous studies with CPDA-1 and Nutricel preserving solutions indicated that red cell properties were affected by small differences in storage temperature. The influence of a 3°C differential on the preservation of the in vitro properties of ADSOL-preserved (AS-1) red blood cells was investigated in a paired study. AS-f red blood cells were stored at 2.5 and 5.5 °C for 42 days. Extent of hemolysis, glucose consumption and pH levels were comparable at the two storage temperatures. Lactate levels were slightly, but significantly higher at 5.5°C. ATP levels were slightly but significantly higher at 2.5°C, only during the later part of the storage period. 2.3-DPG levels were slightly better retained at 2.5 °C after 7 days of storage. Holding units of whole blood for either 1 or 8h at ambient temperature after phlebotomy prior to processing did not influence the types of temperature-dependent changes. The differences as a function of storage temperature were small and appear to be of no practical importance in connection with the storage of AS-1 red blood cells.