Studies on the Procurement of Blood Coagulation Factor VIII

Abstract

Our previous studies have shown that the use of half-strength citrate anticoagulant improves plasma factor VIII stability while maintaining the viability of red cells and platelets. This study extends these observations to show that the stability of factor VIII is maintained in blood stored at room temperature or 4°C overnight and that in vitro tests indicate comparable quality of red cells and platelets prepared from half-strength citrate donations processed immediately or after overnight storage at room temperature. The exception to this was a more rapid loss of red cell 2,3-diphosphoglycerate, as has previously been observed for full-strength citrate donations.

Effect of Whole Blood Storage on Factor VIII Recovery in Fresh Frozen Plasma and Cryoprecipitate

“…We believe that this is due to less efficient cooling to 1-6 C in routine production, which is in agreement with the findings of Rock and Tittley [8]. Griffin et al [9] also found no statistical difference for FVIII recovery in plasma when whole blood was stored at 4 C or at room temperature, which was probably also due to the inefficient cooling at 1-6 C.…”