Survivability of Penaeus monodon during white spot syndrome virus infection and its correlation with immune related genes

Jeswin, Joseph; Anju, A; Thomas, P C; Paulton, M P; Vijayan, K.

doi:10.1016/j.aquaculture.2012.12.004

Cited by 14 publications

(10 citation statements)

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“…Several of the SNPs directly associated, or closely linked to WSSV resistance QTL, were found to occur in transcripts that share homology to genes with putative immune functions. Some of the genes, such as heat shock protein 21, c-type lectin and serine-threonine specific protein kinase, have been implicated in affecting the WSSV resistance of crustaceans in other studies [ 18 , 20 , 43 – 45 ]. Some are components of gene pathways, such as the ubiquitination pathway, which have been found to affect the pathogenesis of WSSV [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…The innate immune response of shrimp has been shown to be triggered almost instantaneously in response to peptidoglycan stimulation [15] and is believed to be the primary defence mechanism against infection in this group of species. A number of potential antimicrobial peptide coding genes have been isolated from penaeid shrimp and some such as penaeidins and crustins have been found to be differentially expressed over the time course of infection [16–18]. The susceptibility of P. monodon to white spot disease has been shown to increase when penaeidin class 5 expression is suppressed by interference mediated gene silencing [19].…”

Section: Introductionmentioning

confidence: 99%

“…The susceptibility of P. monodon to white spot disease has been shown to increase when penaeidin class 5 expression is suppressed by interference mediated gene silencing [ 19 ]. Shrimp surviving 84 hours post-infection have higher expression of lysozyme, C-type lectin, penaeidins, prophenoloxidase-1 and prophenoloxidase-2 in haemocytes than those dying less than 60 hours post infection [ 18 ]. Heat shock protein 21 is down regulated after infection to WSSV [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon)

et al. 2014

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BackgroundShrimp culture is a fast growing aquaculture sector, but in recent years there has been a shift away from tiger shrimp Penaeus monodon to other species. This is largely due to the susceptibility of P. monodon to white spot syndrome virus disease (Whispovirus sp.) which has impacted production around the world. As female penaeid shrimp grow more rapidly than males, mono-sex production would be advantageous, however little is known about genes controlling or markers associated with sex determination in shrimp. In this study, a mapped set of 3959 transcribed single nucleotide polymorphisms were used to scan the P. monodon genome for loci associated with resistance to white-spot syndrome virus and sex in seven full-sibling tiger shrimp families challenged with white spot syndrome virus.ResultsLinkage groups 2, 3, 5, 6, 17, 18, 19, 22, 27 and 43 were found to contain quantitative trait loci significantly associated with hours of survival after white spot syndrome virus infection (P < 0.05 after Bonferroni correction). Nine QTL were significantly associated with hours of survival. Of the SNPs mapping to these and other regions with suggestive associations, many were found to occur in transcripts showing homology to genes with putative immune functions of interest, including genes affecting the action of the ubiquitin-proteasome pathway, lymphocyte-cell function, heat shock proteins, the TOLL pathway, protein kinase signal transduction pathways, mRNA binding proteins, lectins and genes affecting the development and differentiation of the immune system (eg. RUNT protein 1A). Several SNPs significantly associated with sex were mapped to linkage group 30, the strongest associations (P < 0.001 after Bonferroni correction) for 3 SNPs located in a 0.8 cM stretch between positions 43.5 and 44.3 cM where the feminisation gene (FEM-1, affecting sexual differentiation in Caenorhabditis elegans) mapped.ConclusionsThe markers for disease resistance and sexual differentiation identified by this study could be useful for marker assisted selection to improve resistance to WSSV and for identifying homogametic female individuals for mono-sex (all female) production. The genes with putative functions affecting immunity and sexual differentiation that were found to closely map to these loci provide leads about the mechanisms affecting these important economic traits in shrimp.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-731) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon)

et al. 2014

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“…Hence, we speculate that PmVRP15 is not universally associated with WSSV susceptibility in other populations besides the Thai population. Furthermore, there are extensive studies indicating that WSSV resistance and WSSV susceptibility in penaeid shrimps is synergic immune response regulated by many genes (Ghosh et al, 2011), such as anti-lipopolysaccharide factor (ALF), which plays a role in defense against WSSV (Liu et al, 2014;Wang et al ,2013), Penaeus monodon antiviral (PmAV), which presumably acts as an intermediate factor during antiviral reaction (Luo et al, 2003), and other antimicrobial peptides like penaeidin class 5 that protect shrimps at early infection of WSSV (Jeswin et al, 2013;Wang et al, 2013). Perhaps WSSV susceptibility Al Haq et al in G 8 and G 8iA can be marked with other considerable immune-related gene expression rather than PmVRP15.…”

Section: Fig 2 Cumulative Mortality Of P Monodon Fabricius In G 8 Amentioning

confidence: 99%

“…WSSV has been reported to cause harvest failure with a 100% morbidity and mortality level in three to ten days (Jeswin et al, 2013;Reddy et al, 2013). This virus is an enveloped, double-stranded DNA virus that is classified into genus Whispovirus and family Nimaviridae (Tonganunt et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

et al. 2017

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White spot syndrome virus (WSSV) is a devastating viral disease in shrimp aquaculture. Infection of WSSV in penaeid shrimps affects immune defense and changes gene expression. PmVRP15 has been reported as a part of the WSSV propagation pathway that is highly up-regulated in hemocytes at the acute phase of WSSV infection. This study analyzed the expression of PmVRP15 in local populations of giant tiger shrimp (Penaeus monodon) to be associated with susceptibility to WSSV. Tested populations consisted of an inbreeding population (G 8 ) and outbreeding population (G 8iA ) from Jepara, Indonesia. Susceptibility was determined by cumulative mortality, median lethal time (LT 50 ), and severity of infection at time of death. Though all populations were susceptible to WSSV, the first mortality in G 8 occurred at 18 hours post-infection (hpi) with mild infection, while first mortality of G 8iA occurred at 30 hpi with severe infection. The LT 50 of G 8 was significantly lower than that of G 8iA , indicating that G 8iA was less susceptible to WSSV than G 8 . Relative PmVRP15 transcripts of G 8iA were insignificantly down-regulated, whereas relative PmVRP15 transcripts of G 8 were insignificantly upregulated. Although it's still not conclusive, the results of this study suggest that PmVRP15 has weak potential as a WSSV susceptibility marker in G 8 and G 8iA broodstock selection.

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Temperature induced changes in physiological traits and expression of selected candidate genes in black tiger shrimp (Penaeus monodon) larvae

Rahi

Mahmud

Dilruba

et al. 2021

Aquaculture Reports

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Survivability of Penaeus monodon during white spot syndrome virus infection and its correlation with immune related genes

Cited by 14 publications

References 50 publications

QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon)

QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon)

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

Temperature induced changes in physiological traits and expression of selected candidate genes in black tiger shrimp (Penaeus monodon) larvae

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