Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil

Abstract

Although anemia has been historically linked to Haemonchus contortus infection, other infectious agents, such as hemotropic mycoplasmas and tick-borne disease pathogens, may also lead to anemic crisis in sheep. This study has aimed to investigate infections related to anemia in a sheep herd from Bandeirantes City, Paraná State, southern Brazil. Seven out of forty-two (16.6%; 95% CI: 8.32–30.6%) sheep were positive for hemoplasmas by a PCR targeting the 16S rRNA gene and all tested negative for A. marginale/A. ovis and Babesia/Theileria spp. by PCR based on msp4 and 18S rRNA genes, respectively. Two (4.7%; 95% CI: 1.32–15.79%) animals were infested with Rhipicephalus microplus ticks. Fecal egg counting was performed in 38 sheep and 24 (63.15%; 95% CI: 47.2–76.6%) presented > 500 eggs per gram. Phylogenetic analysis of partial sequences of the detected hemotropic Mycoplasma sp. 16S and 23S rRNA genes confirmed that the animals were infected with Mycoplasma ovis. Polymorphism analysis of partial 16S rRNA sequences showed three different genotypes of M. ovis infecting sheep assessed in the present study. Mycoplasma ovis and gastrointestinal nematodes occurs in sheep from the northern region of Paraná State.

Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil

“…(5′-GGACAGAATTTACCTGACAAGG-3′), described by Mongruel et al (2020). The amplification mixture contained 1× PCR buffer, 1.5 mM of MgCl 2 , 0.2 mM of each dNTP, 0.4 mM of each primer, 2.5 U of Taq Platinum DNA Polymerase (Life Technologies ™ ), 5 μl of DNA template, and ultrapure water q.s.p 25 μl.…”

‘ Candidatus Mycoplasma haematonasua’ and tick‐borne pathogens in ring‐tailed coatis ( Nasua nasua Linnaeus, 1976) from the Iguaçu National Park, Paraná State, southern Brazil

“…The gapdh gene was consistently amplified in all ring-tailed coati (Barker et al, 2011;Pontarolo et al, 2021;Valente et al, 2020;Vieira et al, 2021). On this pattern, the 23S rRNA sequences amplified in the present study showed only 94.3%-94.1% identity to 'Ca.…”

“…of the 16S rRNA gene of haemoplasmas (Hoelzle et al, 2011;Machado et al, 2017). Ring-tailed coatis' DNA samples that showed to be positive in the cPCR assay based on the 16S rRNA were submitted to genus-specific cPCR assays targeting fragments of the 23S rRNA (800 pb) (Mongruel et al, 2020) and RNaseP (170 bp) genes (Compton et al, 2012) of haemoplasmas. Nuclease-free water and Mycoplasma ovis DNA obtained from a naturally infected goat (Capra hircus) blood sample were used as negative and positive controls, respectively, in both cPCR assays.…”

‘CandidatusMycoplasma haemosphiggurus’ a novel haemoplasma species in orange‐spined hairy dwarf porcupines (Sphiggurus villosus) from Southern Brazil

“…Thereafter, DNA samples were screened for haemotropic mycoplasmas using a PCR assay targeting a fragment (∼900 bp) of the 16S rRNA gene of haemoplasmas (Hoelzle et al., 2011; Machado et al., 2017). Porcupine DNA samples that tested positive in the PCR assay based on the 16S rRNA gene were subjected to a genus‐specific PCR assay targeting a fragment (800 pb) of the 23S rRNA gene of haemoplasmas (Mongruel et al., 2020). Mycoplasma ovis DNA obtained from a naturally infected goat (Machado et al., 2017) and nuclease‐free water were used as positive and negative controls, respectively.…”