Survey of Theileria lestoquardi antibodies among Sudanese sheep

Salih, Diaeldin A.; Elhussein, A. M.; Hayat, Mohsin; Taha, Khalid M.

doi:10.1016/s0304-4017(02)00389-8

Cited by 38 publications

(22 citation statements)

References 11 publications

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“…Since Khuzestan is known as an endemic site for both of bovine and ovine theileriosis and also these animals are raised together in some part of this province, our finding about mixed infection of T. lestoquardi and T. annulata in sheep in this area is reasonable. The presence of antibodies against T. annulata has been demonstrated in naturally infected Sudanese sheep sera by IFAT (Salih et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR–RFLP

et al. 2010

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Ovine theileriosis is an important hemoprotozoal disease of sheep and goats in tropical and subtropical regions that leads to economic losses in these animals. A nested PCR-restriction fragment length polymorphism (RFLP) was carried out to identification Theileria species in sheep in some area in western half of Iran (Sari, Rasht, Urmia, Ilam, and Ahvaz). Two hundred and fifty blood samples were taken from sheep during tick activating season (summer of 2008). Microscopic examination revealed that 9.2% (23/250) sheep were infected by Theileria spp. piroplasms. Parasitemia ranged from 0.011% to 0.015%. In nested PCR assessment of DNA samples, 32.8% (82/250) sheep were positive. The negative samples were confirmed by amplifying of ovine beta-actin gene as an internal control. The differentiation of Theileria species was based on RFLP patterns using three restriction enzymes: HpaII, Rsa1, and Bsh 1285I. Out of 82 positive samples, 54.8% (45/82) and 40.2% (33/82) were positive for Theileria lestoquardi and Theileria ovis respectively. Mixed infection was detected in 4.8% (4/82) cases. Based on their PCR product digestion pattern with HpaII (1178, 900, 278, and 106 bp), it seemed to be mixture of Theileria annulata and T. lestoquardi. The presence of T. annulata was supported by sequence analysis. This is the first report of naturally infected sheep with T. annulata in Iran. Geographical distribution of Theileria species in sheep is shown according to the result of microscopy and nested PCR and RFLP data.

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Section: Discussionmentioning

confidence: 99%

Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR–RFLP

et al. 2010

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“…However, field application for these techniques is not documented, possibly due to the elevated application costs. The indirect fluorescent antibody test (IFAT) based on T. lestoquardi schizont antigen has been described (Hawa et al 1981;Leemans et al 1997;Salih et al 2003;Taha et al 2003), however, its vast limitations render its routine use in large scale epidemiological surveys impractical. These limitations are mainly due to its tediousness, the subjectivity of the results obtained, and the absence of a means to standardize, thus making the test unsuitable for automation.…”

Section: Introductionmentioning

confidence: 99%

A new recombinant protein-based ELISA for the diagnosis of malignant theileriosis of sheep and goats

2005

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Tick-borne diseases of small ruminants are of highly economic importance in many countries. Malignant theileriosis of sheep and goats caused by Theileria lestoquardi is considered among the most important of these diseases and constitutes an obstacle to the sheep industry in countries like the Sudan. Here the application of a newly discovered surface protein of T. lestoquardi (Clone-5) in an enzyme-linked immunosorbent assay (ELISA) and the potentials of the application of the test in epidemiological surveys and diagnosis are described. Clone-5 contains a predicted number of 20 antigenic determinant sites and two polypeptides derived from the protein were recombinantly produced, purified and tested with control serum samples in both ELISA and Western Blot. One of the polypeptides was further used in validation experiments that involved the testing of negative and positive field serum samples collected from an area that had witnessed an outbreak of malignant theileriosis in Northern Sudan. ELISA, based on this recombinant protein, demonstrated a satisfactory performance with a calculated sensitivity and specificity of 94.6 and 88%, respectively, when countertested with a standard indirect fluorescent antibody test (IFAT). Moreover, no cross-reactions could be demonstrated against Theileria species (China) nor Cowdria spp. This test is recommended for further field validation experiments.

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“…Moreover, antibodies against T. annulata have 312 been detected in sera of naturally infected sheep (Salih et al, 2003 (Brown et al, 1998). Additionally, it has been reported that T. annulata mixed infection of these two species were detected at a lower prevalence of 6.6% than 325 single species infections (Rashidi and Razmi, 2013).…”

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confidence: 99%