Molecular surveillance of Theileria parasites of livestock in Oman

Al-Fahdi, Amira; Al-Qamashoui, Badar; Al-Hamidhi, Salama; Köse, Onur; Tageldin, Mohammed H.; Bobade, P.A.; Johnson, Eugene H.; Hussain, Abdel Rahim; Karagenç, Tülin; Tait, Andy; Shiels, Brian; Bilgiç, Hüseyin Bilgin; Babiker, Hamza A.

doi:10.1016/j.ttbdis.2017.05.008

Cited by 26 publications

(26 citation statements)

References 39 publications

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“…Thus, a mixture of both species was detected in the majority of blood samples. The lower prevalence of single T. ovis differs from an earlier survey in Oman, (April and August 2014) when single T. ovis infection was more dominant than single T. lestoquardi 6 . The lower prevalence of single T. ovis among the present cohort is unlikely to be due to technical deficiency generating false negative results, as the limit of detection of the T. ovis specific qPCR assay was two-fold higher than that of T. lestoquardi.…”

Section: Analysis Of T Lestoquardi and T Ovis Infected Sheep Fiftycontrasting

confidence: 86%

Dynamics and within-host interaction of Theileria lestoquardi and T. ovis among naive sheep in Oman

Awad

Gadalla

Postigo

et al. 2020

Sci Rep

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Mixed species infections of Theileria spp. are common in nature. Experimental and epidemiological data suggest that mixed species infections elicit cross-immunity that can modulate pathogenicity and disease burden at the population level. The present study examined within-host interactions, over a period of 13 months during natural infections with two Theileria spp., pathogenic (T. lestoquardi) and non-pathogenic (T. ovis), amongst a cohort of naive sheep in Oman. In the first two months after exposure to infection, a high rate of mortality was seen among sheep infected with T. lestoquardi alone. However, subsequently mixed-infections of T. lestoquardi and T. ovis prevailed, and no further death occurred. The overall densities of both parasite species were significantly higher as single infection vs mixed infection and the higher relative density of pathogenic T. lestoquardi indicated a competitive advantage over T. ovis in mixed infection. The density of both species fluctuated significantly over time, with no difference in density between the very hot (May to August) and warm season (September to April). A high degree of genotype multiplicity was seen among T. lestoquardi infections, which increased with rising parasite density. Our results illustrate a potential competitive interaction between the two ovine Theileria spp., and a substantial reduction in the risk of mortality in mixed parasite infections, indicating that T. ovis confers heterologous protection against lethal T. lestoquardi infection.

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Section: Analysis Of T Lestoquardi and T Ovis Infected Sheep Fiftycontrasting

confidence: 86%

Dynamics and within-host interaction of Theileria lestoquardi and T. ovis among naive sheep in Oman

Awad

Gadalla

Postigo

et al. 2020

Sci Rep

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“…They found that T. annulata infection was more prevalent in indigenous animals. The molecular techniques are more effective in detecting T. annulata infection and have higher specificity in comparison with other techniques [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Theileria annulata in dairy cattle in Nyala, South Darfur State, Sudan

Abaker¹,

Salih²,

Haj³

et al. 2017

Vet World

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Aim:This study was conducted in dairy cattle in Nyala, South Darfur State, during the period from June to September 2015, to study the prevalence of bovine tropical theileriosis.Materials and Methods:Apparently, healthy cattle of different age groups, different breeds, and from both sexes were randomly selected from seven locations. Three age groups of cattle were selected, group one <1 year old, group two 1-3 years old, and group three older than 3 years. These cattle were indigenous and cross (Friesian X zebu). A total of 150 blood samples were collected for blood smears, blood in EDTA tubes, and serum samples as well as ticks infesting cattle. Three diagnostic techniques were used such as blood smear, indirect fluorescent antibody test (IFAT), and polymerase chain reaction (PCR).Results:Of 150 samples, 11 (7.3%, 95% confidence interval [CI]: 9.1-5.5) were positive for Theileria spp. piroplasms in the blood smears, 70 (46.7%, 95% CI: 35.7-57.7) were positive for Theileria annulata antibodies in the IFAT, and of 100 samples, 39 (39%, 95% CI: 46.6-31.4) were positive for T. annulata using PCR. The prevalence of T. annulata was higher in indigenous breed than cross cattle by the three diagnostic techniques. The highest prevalence of T. annulata was recorded among cattle older than 3 years old. There were three genera and ten species of ticks found feeding on cattle. These were Rhipicephalus evertsi evertsi, Rhipicephalus decoloratus, Rhipicephalus annulatus, Hyalomma dromedrii, Hyalomma impeltatum, Hyalomma rufipes, Hyalomma anatolicum, Hyalomma truncatum, Amblyomma variegatum, and Amblyomma lepidum.Conclusion:The study concluded that tropical theileriosis is prevalent among dairy cattle in Nyala. H. anatolicum was found in very low numbers, suggesting other ticks may play a role in the transmission of the disease. Molecular characterization of T. annulata is recommended for accurate mapping of the disease and evaluates the magnitude problem of tropical theileriosis in South Darfur region.

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“…Tick-borne diseases of cattle such as theileriosis and babesiosis are important due to the losses they cause to animal health and industries (2). These diseases record high presence in the Arabian Gulf regions and place severe impact on their economies (3). Ticks can be transmitted and spread out by various ways including but not limited to moving of animals from an area to another searching for food and water.…”

Section: Introductionmentioning

confidence: 99%

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

Alfatlawi¹,

Ali²,

Albayati³

2019

IJVS

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Studies had been previously conducted to genetically identify species of ticks in Iraq. Therefore, the current investigational study was conducted to recognize the species of 50 ticks collected from infested skin of cattle. The current study defined the ticks to be from Hyalomma genus depending on their morphological features. Using mitochondrial cytochrome oxidase subunit  (Cox) gene, 16 ticks were further confirmed using polymerase chain reaction (PCR). Two PCR products were subjected to DNA sequencing to name the species of the ticks and compare them to some other known ticks in neighbor and world countries. The sequencing results identified the ticks to be Hyalomma anatolicum. One isolate is closely similar to Indian and Iranian isolates, and the other isolate is clustered alone by itself. The results indicated that H. anatolicum is one of the widespread ticks that affect cattle in Al Najaf province, Iraq.

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Molecular surveillance of Theileria parasites of livestock in Oman

Abstract: 10 Background 11Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian 12 Peninsula, and causes high rates of mortality and morbidity in sheep and cattle.

Cited by 26 publications

References 39 publications

Dynamics and within-host interaction of Theileria lestoquardi and T. ovis among naive sheep in Oman

Dynamics and within-host interaction of Theileria lestoquardi and T. ovis among naive sheep in Oman

Prevalence of Theileria annulata in dairy cattle in Nyala, South Darfur State, Sudan

Morphological and phylogenetic study of Hyalomma anatolicum in Al-Najaf, Iraq

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