This manuscript analyze the KAP about the infection by the Zika virus between navigators from Portuguese Navy ships. We developed a statistical analysis, a cross-sectional study that, splitting distinct groups under study those who will navigate in endemic areas of Zika virus, those that have traveled to endemic areas of ZIKV and navigators in non-endemic areas of ZIKV. The present study allows to describe knowledge, attitudes and practices related to ZIKV infection, also let us to stratify the different groups under study: those who will navigate in endemic areas of Zika virus, those that have traveled to endemic areas of ZIKV and navigators in non-endemic areas of ZIKV. The knowledge level about ZIKV reveals significant differences between the distinct groups. The preliminary results are similar other performed studies revealing an urgency to implement an educational health program about Zika infection, previous to a future outbreak by Zika virus.