Survey of Porcine Rotavirus G and P Genotype in Poland and the United States Using RT‐PCR

Abstract

Porcine rotaviruses are a common cause of gastroenteritis. Several serotypes have been detected based on the two surface proteins VP4 (P-types) and VP7 (G-types). However, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. In this study, we describe a reverse transcriptase-polymerase chain reaction (RT-PCR) method for the typing of P and G types of rotavirus. This method allowed to detect G and P types in 96.8 and 87.1% of isolates collected in the United States, respectively and in 54.5 and 38.6% of isolates collected in Poland, respectively. Within the US specimens the G3, G4, G5, G9 and G10 types were detected in combination with P6 and P7 types while among Polish specimens only G3, G4 and G5 types in combination with P6 and P7 types were identified. In both instances the G4 and G5 were the most prevalent types. These studies show that a RT-PCR typing method is suitable for molecular epidemiological studies and that there is more diversity among porcine rotavirus than previously reported.

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

“…The most prevalent genotypes, G3P[6], G4P[6] and G5P [7], were also the most prevalent in other studies throughout the world (Barreiros et al, 2003;Estes, 2001;Winiarczyk et al, 2002). We detected the recently described new rotavirus genotype P[27] in 11.1 % of all positive samples.…”

Multiple Combinations of P[13]-Like Genotype with G3, G4, and G5 in Porcine Rotaviruses

“…The specimens were evaluated for the presence of group A rotavirus by enzyme-linked immunosorbent assay (ELISA) with a polyclonal antibody against group A rotavirus as the capture antibody (15). The samples that were positive for group A rotavirus by ELISA were investigated further for their G and P genotypes by multiplex reverse transcription-PCR (RT-PCR), as described elsewhere (4,6,9,10,11,24,31,40).…”

“…In addition, other G and P types, such as G1, G2, G6, G8, G9, and G10 and P [13] (1,3,5,9,10,16,18,27,29,33,34,37,39,40,41). Accordingly, a comprehensive genotypic characterization of the rotavirus strains circulating in domestic animal populations, especially pigs, is important to define the extent of rotavirus diversity.…”

“…Several epidemiological studies have demonstrated that among porcine rotaviruses (PoRVs), G3, G4, and G5, are the most common G genotypes and usually associate with the P [6] or P [7] genotype (5,23,39,40). In addition, other G and P types, such as G1, G2, G6, G8, G9, and G10 and P [13], P [19], P [23], P [26], and P [27], have also been identified in various geographical settings (1,3,5,9,10,16,18,27,29,33,34,37,39,40,41).…”

Coronavirus em aves: detecção, caracterização molecular e filogenética e inoculação experimental em aves SPF

“…Ainda, é necessário que se considere a ocorrência de falhas na reação de PCR em si: perda de material nas etapas de extração de RNA e/ou de transcrição reversa, mudanças sucessivas na temperatura devido a congelamento e descongelamento das amostras, levando à degradação da partícula viral e seu RNA, ação de inibidores inespecificos como RNAses. Devese levar em conta também, a possibilidade de excesso de DNA/RNA na amostra (RASOOL et al, 2002;WINIARCZYK et al, 2002). A partir deste momento experimental e até o final da primeira quinzena do experimento, os sintomas observados predominantemente nas aves dos grupos experimentais 1, 2, 3 e 4 foram compatíveis com processos patológicos entéricos, como por exemplo, despigmentação em pernas e bico, palidez em cristas e barbelas, depressão, penas arrepiadas, assim como heterogeneidade no crescimento no grupo 1 e 3 sintomas que podem ser associados à má absorção primaria, ou seja, por perda de vilosidades no intestino delgado (KAPIKIAN, 1994).…”