Survey of campylobacter, salmonella and mycoplasmas in house crows (Corvus splendens) in Malaysia

Abstract

House crows (Corvus splendens) in Selangor, Malaysia were examined for the presence of Campylobacter species, Salmonella species, Mycoplasma gallisepticum and Mycoplasma synoviae by serology, culture and pcr. For the detection of Campylobacter and Salmonella species swabs were taken either from the intestine or cloaca. For the detection of mycoplasmas, swabs were taken either from the choanal cleft or trachea for culture and pcr and serum samples were tested by the rapid serum agglutination (rsa) and monoclonal antibody-blocking elisa (mbelisa) for antibodies to M gallisepticum and M synoviae. For campylobacter, 25.3 per cent of the crows were positive by culture, and the species identified were Campylobacter jejuni and Campylobacter coli. No Salmonella species were isolated. Four of 24 swabs were positive for M gallisepticum dna but none gave positive results for M synoviae dna. No M gallisepticum or M synoviae antibodies were detected by rsa but 60 per cent of the sera gave positive reactions for M gallisepticum and 13 per cent gave positive reactions for M synoviae by mbelisa.

Evidence of Carriage of Antimicrobial Resistant Salmonella species of Public Health and Veterinary Significance in the Intestines of House Crows (Corvus splendens) in Tanzania

“…from 6.7% of the cloacal swabs taken from apparently healthy crows in Japan(n=30). Contrary to these findings, other studies conducted elsewhere [11,12] couldn't isolate Salmonella from the crows. The isolation of the two serovars of Salmonella (S. Gallinarum and S. Typhi) from the intestines of Indian house crows in this study confirms the role of these birds as potential carriers of enteric Salmonellafor both humans and animals [13,14].…”

Determining the pathogenicity of bacteria present in wetland waters and crow feces in Bothell

“…Our detection of Campylobacter and E. coli in the crows roosting around the wetland restora-tion area is consistent with similar studies conducted in other suburban areas of other countries, namely in Japan, New Zealand, Malaysia, and Tanzania 7,8,9 . In addition, we also detected virulent genes in Campylobacter which suggests that these bacteria are pathogenic and can cause diarrheal disease in humans.…”

“…have also been isolated from crows and other avian reservoirs in previous studies 7,8,9,10,11 . Therefore, these bacteria will be our species of interest in determining the pathogenic potential, which will be isolated from the water samples and crow feces in the wetland restoration area.…”

Microbiota cloacal aeróbia de cracídeos cativos no Rio Grande do Sul e sua susceptibilidade a antimicrobianos

“…Aves silvestres podem servir de reservatórios dessa bactéria, como foi observado na Noruega em um surto de salmonelose em humanos associado às aves aquáticas (Refsum et al 2002). No presente estudo, não foi obtido nenhum isolado de Salmonella spp.. A prevalência de salmonelas em aves silvestres sadias é baixa, o que pode conferir resultados negativos, como observado no presente estudo e em outros (Kapperud & Rosef 1983, Brittingham et al 1988, Ganapathy et al 2007. Também o método de colheita pode interferir nos resultados, uma vez que swabs cloacais de aves podem não ser efetivos para detectar as bactérias quando estas estão em número reduzido (Bichler et al1996), o que pode explicar os resultados negativos obtidos neste estudo.…”

“…e Listeria spp. também foram isolados dessas aves (Steele et al 2005, Epstein et al 2007, Ganapathy et al 2007). Além disso, já foi relatada a possibilidade de transmissão de patógenos, como Salmonella spp.…”