Survey for β-lactamase among bacterial isolates from Guangzhou, China hospitals between 2005–2006

Wei-jiao, Liao; Jie-hua, Jiang; Xu, Yang; Yi, Jian-yun; Chen, Tao; Su, Xiuxin; Pan, Shaowei; Wei, Xiao-Ping; Li, Yiquan

doi:10.1038/ja.2010.23

Cited by 10 publications

(5 citation statements)

References 12 publications

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“…The strains were also analyzed for the presence of other major beta-lactamase genes ( bla CTX-M , bla SHV , bla TEM ), plasmid mediated quinolone resistance (PMQR) determinants ( qnr A, qnr B and qnr S), sulfonamide resistance genes ( sul I and sul II) and tetracycline resistance genes ( tet A and tet B) [7, 17–22]. To define the mechanism of transmission of the pAmpC gene, we screened bla ampC for the insertion sequence ISE cp 1 by ISE cp 1- bla CMY linkage PCR [23]. Strains positive for this PCR were sequenced as previously described.…”

Section: Methodsmentioning

confidence: 99%

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

et al. 2019

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Background Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli strains isolated from poultry and humans infections share identical cephalosporin resistance, suggesting that transmission of resistance from poultry meat to humans may occur. The aim of this study was to characterize pAmpC-producing E. coli strains isolated from chicken carcasses and human infection in a restrict area and to determine their antimicrobial resistance profiles, and molecular type by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Results A total of 14 pAmpC-producing E. coli strains were isolated, including eight strains from chicken carcasses and six strains from human infections (from urine, tissue and secretion). The bla CMY-2 gene was identified in all pAmpC-producing E. coli strains by polymerase chain reaction (PCR) and DNA sequencing. High percentages of strains resistant to tetracycline, nalidixic acid and sulfamethoxazole-trimethoprim (78–92%) were detected, all of which were considered multidrug-resistant. Among the non-beta-lactam resistance genes, the majority of the strains showed tet A, tet B, sul I and sul II. No strain was considered an extended-spectrum beta-lactamases (ESBL) producer, and the bla TEM-1 gene was found in 2 strains isolated from human infection. Six strains from chicken carcasses and four strains from humans infections were linked to an ISE cp1 -like element. Through MLST, 11 sequence types were found. Three strains isolated from human infection and one strain isolated from chicken carcasses belonged to the same sequence type (ST354). However, considerable heterogeneity between the strains from chicken carcasses and humans was confirmed by PFGE analysis. Conclusion This study showed the prevalence of E. coli strains producing bla CMY-2 linked to ISE cp1 that were present in both chickens and humans in a restricted area. Our results also suggest the presence of a highly diverse strains that harbor pAmpC, indicating no clonal dissemination. Therefore, continuous monitoring and comparative analyses of resistant bacteria from humans and food-producing animals are needed.

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Section: Methodsmentioning

confidence: 99%

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

et al. 2019

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“…These results indicate that β-lactamase is the main resistance mechanism to cephalexin among these isolates. β-lactamase is produced in a wide range of Gram-positive and Gram-negative bacteria with great variety of chemical, physical and enzymological properties (Doran et al 1990;Toroglu et al 2005;Liao et al 2010). However, bacterial isolates No.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Pharmaceutical Wastes in Treated Sewage Effluents by Bacillus subtilis 1556WTNC

Al-Gheethi¹,

Ismail²

2014

Environ. Process.

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The aim of this study was to investigate the potentials for enzymatic biodegradation of pharmaceutical active compounds (β-lactam antibiotics) in treated sewage effluents as a function of β-lactamase produced Bacillus subtilis 1556WTNC. Four β-lactams antibiotics were selected: two of them belong to penicillin's (amoxicillin and ampicillin) and two belong to cephalosporins (cephalexin and cefuroxime); ciprofloxacin (belongs to quinolones) was used as a negative control. The enzymatic biodegradation process was conducted under the optimal conditions for β-lactams production (5.9 log 10 CFU mL −1 ; pH 6.5; temperature 35°C for 12 days) as determined in this research. The maximum biodegradation was 25.03 % at 1 mg mL −1 for amoxicillin, 15.59 % at 0.8 mg mL −1 of ampicillin, 22.59 % at 1 mg mL −1 of cephalexin, 10.62 % at 1 mg mL −1 of cefuroxime, while it was 2.45 % at 0.6 mg mL −1 of ciprofloxacin. B. subtilis 1556WTNC exhibited the potential to produce β-lactamase and biodegrade β-lactam antibiotic genetically and inducibly B. subtilis 1556WTNC could grow and biodegrade β-lactam antibiotics in conditions similar to the characteristics of treated sewage effluents such as pH, temperature, and during short time (12 days), because it was already acclimatized to those conditions. For this reason, treated sewage effluents were used as source to isolate this strain. It can be concluded that B. subtilis 1556WTNC is suitable to remove pharmaceutical residues from the treated sewage effluents and produce effluents at higher quality than that achieved by secondary treatment process.

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“…It was observed that (7.3%) of the tested isolates were beta-lactamase producers. This suggests possible abuse of these drugs, poor hospital attendance and the need for better enlightenment campaign against the use of drug without prescription [17] .In a recent study in China about 26.4% had a β-lactamase phenotype,the β-lactamase genes, which confer multi-drug resistance, are spreading among clinical bacterial isolates [18]. Increase in the beta-lactamase producing S. aureus and E. coli was above 80%.The increasing in prevalence of microorganism, particularly of beta-lactamase producing E. coli and S. aureus and ESLB is alarming situation [19].…”

Section: Resultsmentioning

confidence: 99%

Determination of Mininmum Inhibitary Concentrationof Ciprofloxcin Amoung β-Lactamaste Producing Isolates

R.K.

A.J.²,

Ramina

et al. 2012

AJPS

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Out of 200 clinical samples (blood ,pus,stool,urine,wound and vaginal swabs) were collected from Baghdad hospitals during June2009-January 2010,96 Gram negative and positive bacterial isolates were identified by bacteriological and biochemical tests. Escherichia coli was the dominant bacteria (27.08%), followed by Salmonella typhi (12.5%), Klebsiella pneumoniae (10.4%), Proteus vulgaris (9.3%), and Staphylococcus aureus (8.3%).Antimicrobial susceptibility test was done against ciprofloxacin disk (5μg) by disk diffusion method and the resistant isolates were 22 (23%);distributed into Escherichia coli (10 isolates), Klebsiella pneumoniae (4 isolates), Salmonella typhi (3 isolates), Enterobacter and Shigella flexnerii (2 isolates) and Staphylococcus aureus one isolate. Seven ciprofloxacin resistant isolates were positive for β-lactamase production test (7.3%). Results of minimal inhibitory concentration of ciprofloxacin for positive β-lactamase producing isolates, E. coli 1, Enterobacter 1, Enterobacter 2, K. pneumoniae, S. flexneri, E.coli 2, and S.aureus were (64,32,32,16,16,16,8 μg/ml) respectly

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Survey for β-lactamase among bacterial isolates from Guangzhou, China hospitals between 2005–2006

Cited by 10 publications

References 12 publications

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Biodegradation of Pharmaceutical Wastes in Treated Sewage Effluents by Bacillus subtilis 1556WTNC

Determination of Mininmum Inhibitary Concentrationof Ciprofloxcin Amoung β-Lactamaste Producing Isolates

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