“…According to a Japanese survey of PI animals in dairy farms located in Kanto and westward regions, the proportion of farms with PI was 7.6%, and the proportion of cattle tested as PI was 0.12% [10]. Other groups reported that the proportion of PI in dairy and beef farms in the Tohoku region was 0.38% [6], and that in beef farms in the Kyushu region was 0.18% [2]. Although the annual number of BVD diagnoses that are mandated to be notified to the government was approximately 300−400 over the last five years [17], it is suggested that a large number of hidden PIs exist in Japan because their estimated number are calculated at more than 4,500 from the reported proportion (0.12%) [10] and total number of cattle in Japan (approximately 3.9 million head in 2020) [21].…”