Endemic infections of bovine viral diarrhea virus genotypes 1b and 2a isolated from cattle in Japan between 2014 and 2020

Abstract

Note, Virology Endemic infections of bovine viral diarrhea virus genotypes 1b and 2a isolated from cattlein Japan between 2014 and 2020

Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016–2017

“…[9] and Nishimori et al [15] are in accordance with that in our study and further confirms that subgenotype 1b is the most important BVDV subgenotype endemic in Tokachi Province. Commercial inactivated and live-attenuated vaccines based on BVDV subgenotypes 1a and 2a strains and commercial inactivated vaccines containing BVDV 1b and 2a are available in Japan [20].…”

“…However, Nishimori et al . [ 15 ] showed that the result of subgenotyping based on 5′ UTR region was consistent with that based on the E2 gene region. In contrast, another study reported the mismatch in the results of subgenotyping of 5′ UTR- and E2 gene-based analysis [ 19 ].…”

“…Subgenotyping of 51 samples was performed based on the 5 UTR sequence region in this study, but the E2 protein was the major target of neutralizing antibodies. However, Nishimori et al [15] showed that the result of subgenotyping based on 5 UTR region was consistent with that based on the E2 gene region. In contrast, another study reported the mismatch in the results of subgenotyping of 5 UTR-and E2 gene-based analysis [19].…”

“…This subgenotyping was performed based on the nucleotide sequence of the entire E2 gene. Nishimori et al [15] reported that BVDV 1b and 2a were predominant and subdominant strains, respectively, circulating in some prefectures, including Hokkaido in 2014-2020. This subgenotyping was performed based on the nucleotide sequences of both the 5 UTR and E gene.…”

Application of Flow Cytometry in the Diagnosis of Bovine Epidemic Disease

“…BVDV belongs to the plague virus genus of the Flaviviridae family. The viral genome is a single-stranded RNA, encoding four structural proteins (i.e., C, Erns, E1, and E2) and eight non-structural proteins (i.e., Npro, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B) [ 29 , 30 ]. Dong Kun et al [ 31 ] applied a double antibody sandwich ELISA kit for bovine viral diarrhoea virus and bovine enterovirus antigen to 738 faecal samples collected from five regions in Jilin Province in 2019–2020, respectively, and confirmed some samples with PCR and immunofluorescence tests.…”