Surveillance of bacterial resistance among isolates in Shanghai in 1999

Wang, Fu; Zhu, Demei; Hu, Fupin; Zhang, Yingyuan

doi:10.1007/s101560100019

Cited by 28 publications

(19 citation statements)

References 3 publications

(3 reference statements)

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“…Although recent data showed a decreased in health careassociated infection due to methicillin-resistant S. aureus (MRSA) [3], MRSA still represents a predominant pathogen associated with serious nosocomial infections including pneumonia [4]. In China, more than 60 % of nosocomial S. aureus infections are caused by MRSA [5]. The increasing prevalence of nosocomial infections caused by MRSA has not only led to a higher mortality rate than for infections with other causes [6,7], but also higher morbidity, hospital length of stay (LOS), and medical costs [8,9].…”

Section: Introductionmentioning

confidence: 99%

Linezolid versus vancomycin for the treatment of suspected methicillin-resistant Staphylococcus aureus nosocomial pneumonia: a systematic review employing meta-analysis

Wang

Zou

Xie

et al. 2014

Eur J Clin Pharmacol

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Section: Introductionmentioning

confidence: 99%

Linezolid versus vancomycin for the treatment of suspected methicillin-resistant Staphylococcus aureus nosocomial pneumonia: a systematic review employing meta-analysis

Wang

Zou

Xie

et al. 2014

Eur J Clin Pharmacol

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“…Resistance to quinolones has increased markedly, however, in some areas of the world since their introduction. In China, more than 50% of the clinical strains of Escherichia coli are resistant to ciprofloxacin (27,28).…”

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confidence: 99%

Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Shanghai, China

Wang

Tran

Jacoby

et al. 2003

Antimicrob Agents Chemother

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437

387

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Although quinolone resistance usually results from chromosomal mutations, recent studies indicate that quinolone resistance can also be plasmid mediated. The gene responsible, qnr, is distinct from the known quinolone resistance genes and in previous studies seemed to be restricted to Klebsiella pneumoniae and Escherichia coli isolates from the University of Alabama in Birmingham, where this resistance was discovered. In Shanghai, the frequency of ciprofloxacin resistance in E. coli has exceeded 50% since 1993. Seventy-eight unique ciprofloxacin-resistant clinical isolates of E. coli from Shanghai hospitals were screened for the qnr gene by colony blotting and Southern hybridization of plasmid DNA. Conjugation experiments were done with azide-resistant E. coli J53 as a recipient with selection for plasmid-encoded antimicrobial resistance (chloramphenicol, gentamicin, or tetracycline) and azide counterselection. qnr genes were sequenced, and the structure of the plasmid DNA adjacent to qnr was analyzed by primer walking with a sequential series of outward-facing sequencing primers with plasmid DNA templates purified from transconjugants. Six (7.7%) of 78 strains gave a reproducible hybridization signal with a qnr gene probe on colony blots and yielded strong signals on plasmid DNA preparations. Quinolone resistance was transferred from all six probe-positive strains. Transconjugants had 16-to 250-fold increases in the MICs of ciprofloxacin relative to that of the recipient. All six strains contained qnr with a nucleotide sequence identical to that originally reported, except for a single nucleotide change (CTA3CTG at position 537) encoding the same amino acid. qnr was located in complex In4 family class 1 integrons. Two completely sequenced integrons were designated In36 and In37. Transferable plasmid-mediated quinolone resistance associated with qnr is thus prevalent in quinoloneresistant clinical strains of E. coli from Shanghai and may contribute to the rapid increase in bacterial resistance to quinolones in China.

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“…According to both current and previous reports, the susceptibility of fluoroquinolones (FQs) appears to be less stable, and resistance has developed rapidly. [4][5][6] In this study, the proportion of LVFX-resistant or intermediate strains was over 20% (Fig. 2).…”

mentioning

confidence: 99%

“…[3][4][5][6] In our hospital, total dosages of FQs have been decreasing since 1996. 10 We believe that this has been caused by the biological factors of the organism.…”

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confidence: 99%

Survey of the susceptibility of urinary isolates to antibacterial agents in 2003

Ishikawa

Hayakawa

Miyakawa

et al. 2005

Journal of Infection and Chemotherapy

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Surveillance of bacterial resistance among isolates in Shanghai in 1999

Cited by 28 publications

References 3 publications

Linezolid versus vancomycin for the treatment of suspected methicillin-resistant Staphylococcus aureus nosocomial pneumonia: a systematic review employing meta-analysis

Linezolid versus vancomycin for the treatment of suspected methicillin-resistant Staphylococcus aureus nosocomial pneumonia: a systematic review employing meta-analysis

Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Shanghai, China

Survey of the susceptibility of urinary isolates to antibacterial agents in 2003

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